Viability detection technology for acacia rachii pollen culture in vitro

Abstract

The invention discloses a viability detection technology for acacia rachii pollen culture in vitro. The technology includes the steps that fresh flowering branches comprising multiple inflorescences which bloom on that day and are not touched by insects are collected before 7 am in the morning; the inflorescences are cut into multiple sections, and a culture solution containing cane sugar of 15-20%, boric acid with the concentration of 100-150 mg / L and calcium nitrate with the concentration of 0.25-0.5 mmol / L is added dropwise into a concave slide groove; 2-3 inflorescences are clamped with tweezers and rinsed in the liquid medium by a circle until pollen directly falls into the medium, the medium is not covered with a slide, after sowing, the concave slide is placed into a petri dish where wet filter paper is laid to be cultured, and an optical microscope is used for observing pollen sprouting number through a conventional method after 8 hours. The method is simple, completely quantitative, reliable in data and high in operability, has high practical application value, improves acacia rachii breeding efficiency, effectively solves the yield and quality problems of an existing seed orchard and greatly brings economic benefits, social benefits and ecological benefits of acacia rachii into play.

Description

technical field [0001] The invention belongs to the technical field of plant hybridization and breeding, and relates to a technology for detecting the vitality of acacia pollen in vitro culture. Background technique [0002] Acacia ( Acacia confusa ) belongs to Mimosaceae ( Mimosaceae ) Acacia ( Acacia ) plants, naturally distributed in humid tropical regions such as Queensland, Australia, Papua New Guinea and Indonesia. Acacia has the characteristics of rapid growth, dense canopy, large amount of fallen leaves, root nodules for nitrogen fixation, and resistance to dryness and barrenness. It has excellent materials and can be used as pulp wood, building materials, and furniture materials. Since the introduction of acacia in my country in the 1960s and 1970s, research on the breeding and cultivation of improved acacia species has been paid attention to. Not only have we successfully screened out suitable provenances in various places, but also bred a number of excellent ...

AI Technical Summary

Problems solved by technology

However, some existing problems restrict the yield and quality of seed orchards

For example, the cold damage caused by extreme climate abnormalities, the difficulty of artificial pollination, and the low pod setting rate make the seed garden unable to effectively perform its due functions, which greatly limits the long-term sustainable development and research and utilization of acacia.

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