The invention discloses a
tissue culture and propagation method of eye-spotted
Fritillaria orchid, and relates to the field of eye-spotted
Fritillaria orchid
tissue culture technology. The method includes selecting a robust eye-spotted
Fritillaria orchid
plant, performing artificial cross-
pollination on it during flowering;
cutting off the debris remaining in the fruit pod, washing and disinfecting it and placing it in a sterile dish on a clean bench for standby; dissecting it along the fruit
ridge with a cooling scalpel and
tweezers, taking out the seeds and sprinkling them on the
primary culture medium for dark culture, and light culture after
germination; the germinated eye-spotted Fritillaria orchid continues to grow for 45-60 days, and when the leaves begin to differentiate, the seedlings are transferred to the secondary culture medium for culture for 100-120 days; the sterile seedlings are transferred to the rooting culture medium for rooting culture for 180-210 days; hardening and taming
transplanting. The present invention overcomes the problems of slow traditional division propagation of eye-spotted Fritillaria orchid, incomplete seed
embryo development, and it is difficult for seeds to independently develop into complete plants under natural conditions, while meeting a large number of breeding needs, reducing the excavation of wild resources, and protecting wild resources.