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Dendrobium officinale extract and preparation method thereof

A Dendrobium officinale extract technology, applied in skin care preparations, drug combinations, skin diseases, etc., can solve the problems of low yield of Dendrobium polysaccharides, complicated extraction process, high production cost, etc., achieve good moisturizing activity, simple process, Safe to use

Inactive Publication Date: 2017-05-24
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These patent application documents and literature reports have enriched the extraction and purification methods of Dendrobium officinale polysaccharides, but most of the patent applications and other documents only involve the extraction and purification methods of polysaccharides, mainly including degreasing, water extraction, alcohol precipitation, protein removal, drying and other steps , the yield of Dendrobium polysaccharides by alcohol precipitation is low, and the process of removing protein leads to the loss of some polysaccharides, amino acids and other substances. The overall extraction process is relatively complicated, the yield is low, and the production cost is high.

Method used

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  • Dendrobium officinale extract and preparation method thereof
  • Dendrobium officinale extract and preparation method thereof
  • Dendrobium officinale extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Dendrobium candidum extract:

[0037] The dried Dendrobium candidum sample is pulverized with a pulverizer, passed through a No. 2 sieve, and 300 g of Dendrobium candidum coarse powder is taken, and the decoction method is extracted 2 times, adding 60 times the amount of water of the sample weight for the first time, and adding 60 times of the sample weight for the second time. 30 times the amount of water, the extraction time is 2 hours, combine the filtrate, add 0.5% activated carbon, heat to 80°C, stir for 1 hour, filter, collect the filtrate, concentrate the filtrate to a specific gravity of 1.2 (30°C), and vacuum dry (vacuum Degree is -0.07MPa, temperature is 80 ℃), that is.

[0038] Get Dendrobium officinale extract 158.6g after drying, according to " Chinese Pharmacopoeia " 2010 edition first volume polysaccharide content assay method under Dendrobium officinale item, polysaccharide content is 30.75% (converted into the polysaccharide content meter...

Embodiment 2

[0040] Preparation of Dendrobium candidum extract:

[0041]The dried Dendrobium candidum sample is pulverized with a pulverizer, passed through a No. 2 sieve, and 200 g of Dendrobium candidum coarse powder is taken, and the decoction method is extracted 2 times, adding 80 times of the sample weight in water for the first time, and adding 80 times of the sample weight in the second time. 20 times the amount of water, the extraction time is 2 hours, combine the filtrate, add 0.2% activated carbon, heat to boiling, stir for 1 hour, filter, collect the filtrate, concentrate the filtrate to a specific gravity of 1.1 (30 ° C), microwave drying (power 600W , heating temperature 50 ℃, automatic temperature control 80 ℃), that is.

[0042] Get Dendrobium officinale extract 108.7g after drying, according to " Chinese Pharmacopoeia " 2010 edition first part polysaccharide content determination method under the Dendrobium officinale item, polysaccharide content is 28.92%, and extraction r...

Embodiment 3

[0044] Preparation of Dendrobium candidum extract:

[0045] The dried Dendrobium candidum sample is pulverized with a pulverizer, passed through a No. 2 sieve, and 400 g of Dendrobium candidum coarse powder is taken, and the decoction method is extracted 2 times, adding 40 times of the sample weight in water for the first time, and adding 40 times of the sample weight in the second time. 40 times the amount of water, the extraction time is 2 hours, combine the filtrate, add 0.3% activated carbon, heat to 70°C, stir for 1 hour, filter, collect the filtrate, concentrate the filtrate to a specific gravity of 1.05 (30°C), and spray the concentrate Dry (inlet air temperature 180°C, feed rate 200ml / h) to obtain.

[0046] After drying, 212.3 g of the Dendrobium officinale extract was obtained. According to the polysaccharide content determination method under the item of the first Dendrobium officinale of "Chinese Pharmacopoeia" 2010 edition, the polysaccharide content was 30.42%, an...

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Abstract

The invention relates to a Dendrobium officinale extract and a preparation method thereof. The extract and preparation method thereof extract is prepared from extract and preparation method thereof through drying, crushing, water decoction process extraction, filtration, active carbon decolorizing purification, filtration, concentration and drying. The extract is rich in extract and preparation method thereof polysaccharides and amino acids, and also contains mineral matters, phlegmatic temperament and other components, and all the components are additives commonly used in cosmetics, so the extract can be applied to cosmetics, has the efficacy of moisturizing and nourishing skins, is safe and has no toxic or side effects. The preparation method has the characteristics of simple extraction purifying process, no organic solvents, safety, environmental protection and low production cost.

Description

[0001] Technical field: [0002] The invention belongs to the field of daily chemical products, and specifically relates to an extract of Dendrobium officinale and a preparation method thereof, more specifically, to a method for extracting and purifying biologically active polysaccharides and amino acid components in Dendrobium officinale. [0003] Background technique: [0004] Dendrobium officinale Kimura et Migo is the stem of Dendrobium officinale Kimura et Migo, also known as black knot grass. It was first recorded in "Shen Nong's Materia Medica" and listed as the top grade. Because of its special living environment and outstanding nourishing effect, it ranked first among the "Nine Chinese Immortal Herbs". "Chinese Pharmacopoeia" 2010 edition lists it separately, has the effect of clearing heat and promoting dampness, detoxifying and reducing swelling, and is mainly used for damp-heat jaundice, heatstroke diarrhea, stone drench with blood, carbuncle sores, injuries from fa...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61K8/97A61P17/00A61P17/04A61P37/08A61P17/06A61P17/02A61P17/10A61Q19/00A61P31/10A61P29/00A61P39/06A61K47/46A61Q17/02A61Q17/04
Inventor 沈勇李晓波
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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