Application of water-soluble fluorescent carbon dot in detection of heavy metal silver ion content

A technology of fluorescent carbon dots and silver ions, which is applied in the field of silver ion determination, can solve the problems that limit the prospect of wide application, and achieve the effects of high selectivity, high sensitivity, and simple preparation method

Active Publication Date: 2017-05-24
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This limits the prospect of its wide application to some extent

Method used

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  • Application of water-soluble fluorescent carbon dot in detection of heavy metal silver ion content
  • Application of water-soluble fluorescent carbon dot in detection of heavy metal silver ion content
  • Application of water-soluble fluorescent carbon dot in detection of heavy metal silver ion content

Examples

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Effect test

Embodiment 1

[0028] A method for preparing fluorescent carbon dots (CDs), comprising the following steps:

[0029] Step 1, 0.42 g of anhydrous citric acid was dissolved in 25 mL of ultrapure water, and ultrasonically dissolved to obtain a citric acid solution;

[0030] Step 2, inject 536 μL of ethylenediamine into the bottom of the citric acid solution, transfer it to the polytetrafluoroethylene lining of the reaction kettle, and put the reaction kettle into an oven to make it react to obtain a light yellow solution;

[0031] Step 3, centrifuge the light yellow solution, take the supernatant, and dialysis and purify through the dialysis membrane to obtain fluorescent carbon dots.

[0032] Wherein, the temperature of the oven in step 2 is 160-240° C., and the reaction is 2-8 hours. The centrifugation speed in step 3 is 12,000 rpm for 20 minutes. The dialysis membrane dialysis time in step 3 is 24-48 hours.

[0033] The fluorescent carbon dots of the present invention are installed in a cen...

Embodiment 2

[0036] A kind of application of water-soluble fluorescent carbon dot in detection heavy metal silver ion content, comprises the following steps:

[0037] The first step is to prepare fluorescent carbon dot probes. Take 2 μL of fluorescent carbon dots from Example 1 and add them to a centrifuge tube, then add cysteine ​​(final concentration: 0.5 mM), and dilute with BR buffer solution (pH 7.0) To 2 mL, as a carbon dot / cysteine ​​fluorescent probe, excited with 352 nm wavelength light, measure the fluorescence intensity of the carbon dot / cysteine ​​fluorescent probe at a wavelength of 441 nm, denoted as I 0 ;

[0038] In the second step, take another 2 μL of fluorescent carbon dots from Example 1 and add them to the centrifuge tube, then add cysteine ​​(final concentration: 0.5 mM), dilute to 2 mL with BR buffer solution (pH 7.0) as carbon dots 8 copies of / cysteine ​​fluorescent probes were used to detect the effects of different concentrations of silver ions (final concentrat...

Embodiment 3

[0042] Except that cysteine ​​is not added in the first step, other steps are the same as in Example 2.

[0043] The result is as Figure 4 As shown, through the comparison of the fluorescence signal change curves of carbon dots quenched by silver ions under the conditions of no addition of cysteine ​​and addition of cysteine, it can be seen that the synergistic effect of fluorescent carbon dots and cysteine ​​greatly promotes Ag +The fluorescence quenching effect on the fluorescent carbon dots can effectively improve the Ag + Sensitivity of detection.

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Abstract

The invention discloses application of a water-soluble fluorescent carbon dot in detection of heavy metal silver ion content. The method comprises the following steps: taking a 2mu L synthetic carbon dot stock solution, diluting to 2mL with a BR buffer solution to be taken as a fluorescent probe, exciting with 352nm wavelength light, determining fluorescence intensity of the fluorescent carbon dot probe at 441nm wavelength and recording as I0; taking an another fluorescent carbon dot probe, respectively adding different concentrations of silver ion solutions, repeating the determination step, recording as I441, and calculating linear relation between variation of the fluorescence intensity of the fluorescent carbon dot probe before and after the silver ions are added and the silver ion concentration according to the variation of the fluorescence intensity; and adding a to-be-detected sample into the fluorescent carbon dot probe, recording the variation of the fluorescence intensity at the 441nm wavelength, and calculating the mass or concentration of the added silver ions according to the linear relation. The method disclosed by the invention has an accurate heavy metal silver ion detection result, is convenient and efficient, and has good market prospects.

Description

technical field [0001] The invention relates to the field of silver ion determination, in particular to the application of water-soluble fluorescent carbon dots in detecting the content of heavy metal silver ions. Background technique [0002] Heavy metal ion pollution widely exists in water, air, soil, and food, so it is a very serious problem all over the world. Silver ions and their compounds are widely used in electrical-related industries, photography and videography industries, and pharmaceutical industries, and are eventually released into the ecosystem intentionally or unintentionally. Therefore, related technologies that rely on silver have a certain degree of potential harm to the environment. In recent years, there have been related reports pointing out the bioaccumulation and potential toxicity of silver ions to invertebrates, phytoplankton, algal benign bacteria, amphibians and fish. Therefore, rapid and sensitive detection of silver ions is of great signifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 刘国良冯大千宣成磊王伟
Owner YANCHENG INST OF TECH
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