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Rapid propagation method of Gloriosa superba Linn

A fast and lily technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of lily lily seed reproduction, low tuber reproduction coefficient, slow growth, etc., to achieve easy maintenance and management, and improve reproductive efficiency , the effect of fast growth

Inactive Publication Date: 2017-05-31
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems such as difficulty in seed propagation of Lilium jalan, low propagation coefficient of tubers, slow growth and long time in the later stage of tissue culture propagation and transplanting, the present invention provides a method for rapid propagation of Lily jalan, by improving the production process and the formula of the culture medium, improving The reproduction coefficient of Lilium jalan can quickly produce a large number of high-quality tubers to meet the needs of large-scale production of high-quality seedlings

Method used

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  • Rapid propagation method of Gloriosa superba Linn
  • Rapid propagation method of Gloriosa superba Linn
  • Rapid propagation method of Gloriosa superba Linn

Examples

Experimental program
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Embodiment 1

[0024] A. Selection and sterilization of explants: Select stem tips and axillary buds that grow robustly and have not formed flower buds as explants, peel off the outer leaves, cut them into small sections of 1.5-2.0 cm, and use conventional detergent with appropriate amount of detergent After washing with water, disinfect in 0.1% mercuric chloride solution for 7 minutes, then disinfect in 0.3% sodium hypochlorite solution for 5 minutes, then rinse with sterile water for 4 times, each time for 2 minutes, and then use sterile filter paper Blot dry, reserve;

[0025] B. Adventitious bud induction culture: under aseptic conditions, inoculate the small section after the disinfection and sterilization in step A into the following induction medium: MS+6-benzylaminopurine (6-BA) 6.0mg / L+ naphthaleneacetic acid ( NAA) 0.01mg / L + agar 6.5 g / L + sucrose 30g / L, pH=5.8, under the conditions of culture temperature 27℃, light intensity 2000-3000lx, light time 10h / d, induce culture for 40 d...

Embodiment 2

[0030] A. Selection and sterilization of explants: Select stem tips and axillary buds that grow robustly and have not formed flower buds as explants, peel off the outer leaves, cut them into small sections of 1.5-2.0 cm, and use conventional detergent with appropriate amount of detergent After washing with water, sterilize in 0.1% mercuric chloride solution for 8 minutes, then sterilize in 0.3% sodium hypochlorite solution for 4 minutes, then rinse with sterile water for 4 times, each time for 2 minutes, and then use sterile filter paper Blot dry, reserve;

[0031]B. Adventitious bud induction culture: under aseptic conditions, inoculate the small section after step A disinfection and sterilization in the following induction medium: MS+6-benzylaminopurine (6-BA) 7.0mg / L+ naphthaleneacetic acid ( NAA) 0.03mg / L + agar 7.0 g / L + sucrose 30g / L, pH=5.8, under the condition of culture temperature 27℃, light intensity 2000-3000lx, light time 11h / d, induce culture for 30d until expla...

Embodiment 3

[0036] A. Selection and sterilization of explants: Select stem tips and axillary buds that grow robustly and have not formed flower buds as explants, peel off the outer leaves, cut them into small sections of 1.5-2.0 cm, and use conventional detergent with appropriate amount of detergent After washing with water, sterilize in 0.1% mercuric chloride solution for 10 minutes, then sterilize in 0.3% sodium hypochlorite solution for 3 minutes, then rinse with sterile water for 3 times, each time for 2 minutes, and then use sterile filter paper Blot dry, reserve;

[0037] B. Adventitious bud induction culture: under aseptic conditions, inoculate the small section after the sterilization in step A into the following induction medium: MS+6-benzylaminopurine (6-BA) 8.0mg / L+ naphthaleneacetic acid ( NAA) 0.05mg / L + agar 6.8 g / L + sucrose 30g / L, pH=5.8, under the conditions of culture temperature 27℃, light intensity 2000-3000lx, light time 12h / d, induce culture for 20d until explanted ...

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Abstract

The invention relates to a rapid propagation method of Gloriosa superba Linn and belongs to the technical field of plant tissue culture. The method comprises steps as follows: a sterilized Gloriosa superba Linn explant is inoculated to an induction medium, adventitious buds are induced out and cut off for subculture, subculture is performed multiple times until the required number of adventitious buds with stem discs is obtained, the stem discs are cut off and transferred to a tuber culture medium for culture, after the diameter of tubers is 1-1.5 cm, the tubers are directly transplanted to a seed bed and are germinated and emerge after 40-60 days, a large quantity of Gloriosa superba Linn seedlings can be obtained, the seed filling rate can reach 90%, and the survival rate of transplanting is 95% or higher. With the adoption of the method, a key technology for establishing a Gloriosa superba Linn sterility system is realized, the problems of low propagation coefficient and long production cycle of the Gloriosa superba Linn are solved by integrally regulating hormone, nutrition and culture environment, the purposes of high seed filling rate, high propagation speed and stable production technology are achieved, and large-scale, standardized and industrial production of the Gloriosa superba Linn is realized.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation of lily jalan. Background technique [0002] Gloria lily (Gloriosa superba Linn) belongs to the climbing herbaceous plant of the family Liliaceae, which is native to southern Yunnan Province, Hainan Province and the tropical regions of Asia and Africa. There are wild sporadic distributions below the altitude of 1200m in Xishuangbanna, Yunnan Province, my country. The flower posture is peculiar, like a burning flame, and the color is gorgeous and elegant; the flower color is varied and the flowering period is long, it is a herbaceous flower with great ornamental value. Because it is rich in colchicine, its tubers are one of the ideal raw materials for the production of colchicine. The reproduction methods of Gloria lily include seed propagation, tuber vegetative propagation and tissue culture propagation. Because the seed...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 段青王祥宁蒋亚莲崔光芬贾文杰马璐琳杜文文
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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