Tissue culture method suitable for Chinese herbal medicine murraya paniculata seedling

A tissue culture and Chinese herbal medicine technology, applied in the field of plant reproduction, can solve the problem that seedlings cannot be planted and cultivated efficiently, quickly and industrially on a large scale, and achieves the effects of accelerating the speed of popularization and application, thick stems and easy operation.

Active Publication Date: 2017-05-31
HECHI UNIV
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AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to: aim at the above-mentioned existing problems, provide a kind of tissue culture method applicable to the seedling of Chinese herbal medicine Thyme chinensis, this culture method comprises cluster bud induction culture and adventitious root induction culture, and after several rounds of subculture, buds The growth is normal, and the survival rate of hardened seedlings reaches more than 90%, which can significantly improve the survival rate of Thyme thyme seedlings; and can solve the problem that the existing Chinese herbal medicine Thyme thyme seedlings cannot be efficiently and quickly large-scale industrial planting and cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Pretreatment: Take the healthy and full-bodied Chinese herbal medicine Thyme chinensis terminal buds or axillary buds, use a soft brush to add a small amount of detergent to the water and gently wipe the branches and axillary buds, rinse with tap water for 1 hour, and put them in an ultra- In the clean workbench, sterilize with 70% alcohol for 20 seconds, rinse with sterile water for 4 times, sterilize with mercuric chloride with a concentration of 0.1% for 8 minutes, and rinse with sterile water for 5 times; Inoculate in a test tube filled with germination medium for germination culture, inoculate one terminal bud or axillary bud in each test tube, and then place it in the culture room. The germination medium is: MS basal medium + 6-BA 2.0mg / L +NAA0.2mg / L+sucrose 30g / L+agar powder 5.4g / L, pH 5.8; cultured for 5 weeks at a temperature of 25°C, a light time of 12h / d, and a light intensity of 1500 lux, during which time the contaminated materials were removed;

[0024...

Embodiment 2

[0028] (1) Pretreatment: Take the healthy and plump Thyme Thyme seed material, remove the outer testa, sterilize with alcohol with a volume fraction concentration of 65% for 40 seconds on the ultra-clean workbench, rinse with sterile water 3 times, The mass fraction concentration is 0.2% mercuric chloride for 10 minutes, rinsed with sterile water for 3 times; the materials that have been sterilized on the surface are inoculated into culture bottles filled with germination medium for germination culture, and one seedling is inoculated in each culture bottle. Seeds are then placed in the cultivation chamber, and the germination medium is: on the germination medium of seed germination medium MS+6-BA 2.0mg / L+GA30mg / L+sucrose 20g / L+agar powder 5.4g / L, pH 7.0, Place it in the cultivation room for dark cultivation, cultivate for 5 weeks, and count the germination rate with the growth of young leaves as the germination standard;

[0029](2) Clumping bud induction culture: take the ger...

Embodiment 3

[0033] (1) Pretreatment: Take the healthy and full-bodied Chinese herbal medicine Thyme chinensis terminal buds or axillary buds, use a soft brush to add a small amount of detergent to the water, and gently wipe the branches and axillary buds, rinse with tap water for 1.5 hours, and put them in the water for 1.5 hours. In the ultra-clean workbench, sterilize with 75% alcohol for 30 seconds, wash with sterile water for 4 times, sterilize with mercuric chloride with a concentration of 0.2% for 8 minutes, and rinse with sterile water for 5 times; the surface is sterilized Germination culture is carried out by inoculating the material in a test tube filled with germination medium. Each test tube is inoculated with one terminal bud or axillary bud, and then placed in the culture room. The germination medium is: MS basal medium + 6-BA 2.0mg / L+NAA0.2mg / L+sucrose 30g / L+agar powder 5.4g / L, pH 5.8; cultured for 5 weeks at a temperature of 27°C, a light time of 16h / d, and a light intensi...

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Abstract

The invention discloses a tissue culture method suitable for a Chinese herbal medicine murraya paniculata seedling. The method mainly comprises the following steps: cultivating a murraya paniculata seed or terminal bud or axillary bud on a germination culture medium to grow a germination seedling, inoculating on a cluster bud induction medium, breeding, conducting spreading cultivation, subculturing to grow a cluster bud and an adventitious bud, then obtaining a rootless plant, conducting adventitious root induced culture on the rootless plant, hardening the seedling, and then transplanting and cultivating. The culture medium is formed by adding 6-BA and NAA or GA on an MS of the germination culture medium; the cluster bud induction medium is formed by adding 6-BA and NAA or TDZ on the MS of the cluster bud induction medium; an adventitious root induction medium is formed by adding 6-BA and NAA or IBAK on the MS. The leaf of the adventitious bud of the Chinese herbal medicine murraya paniculata is thick, the stem of the adventitious bud of the Chinese herbal medicine murraya paniculata is thick and large, the adventitious bud of the Chinese herbal medicine murraya paniculata has the advantages of low aberration rate and high growth coefficient, the survival rate of the hardening seedling is up to 90%, the adventitious bud of the Chinese herbal medicine murraya paniculata is suitable for being provided as the Chinese herbal medicine murraya paniculata seedling, the cost is low, the pollution is low, and the Chinese herbal medicine murraya paniculata seedling can be produced all through the four seasons, and is suitable for large-scale culture of seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a tissue culture method suitable for the seedlings of the Chinese herbal medicine Thyme japonica. Background technique [0002] Murraya paniculata (L.) Jack., also known as: Wanlixiang, Jiuqiuxiang, Jiushuxiang, is a small tree of the Rutaceae Murraya plant. Its roots, dry leaves and leafy shoots are used as herbal medicine. Trichosanthes medicinal material is widely used, and it is one of the varieties of Gurixiang recorded in the 2010 edition of "Chinese Pharmacopoeia", mainly distributed in Guangxi, Yunnan, Guizhou and other places. It has the effects of promoting qi and blood circulation, dissipating blood stasis and relieving pain, detoxifying and reducing swelling. It is mainly used for epigastric pain, rheumatism arthralgia, swelling and pain from falls, sores and carbuncles, and snake bites; it is also used for anesthesia and pain relief. Trichosant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 覃国乐史沉鱼覃珍妮李晓东辛磊谢彦军
Owner HECHI UNIV
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