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Preparation method of gallate with high content of epigallocatechin

A technology of gallic acid ester and gallic acid, which is applied in the field of natural product catechin preparation, can solve the problems of easy oxidation of epigallocatechin gallate, low content of purified substance and large amount of solvent, etc. The effect of small solvent throughput, high purity and short crystallization time

Inactive Publication Date: 2017-05-31
HUBEI ZHONGXIN BIOTECH CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing high-content epigallocatechin gallate, which overcomes the cumbersome technical operation of the related purification epigallocatechin gallate, the large amount of solvent, and the The disadvantages of gallate purification are easy to oxidize and the content of the purified product is low

Method used

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Effect test

Embodiment 1

[0019] A kind of preparation method of high-content epigallocatechin gallate according to the embodiment of the present invention 1 comprises the following steps:

[0020] Step 1: Take 2 kg of tea extract containing 70% epigallocatechin gallate, and dissolve the tea extract in 80 liters of 60°C pure water to obtain a tea polyphenol extract solution;

[0021] Step 2: adding the tea extract solution into the chromatographic column to control the adsorption flow rate;

[0022] Step 3: After loading the column, inject 15% ethanol into the chromatographic column and begin to collect the eluate. The collection volume of the eluate is 5BV.

[0023] Step 4: Concentrate the collected eluent under reduced pressure or a single-effect concentrator under reduced pressure, and concentrate its volume to 2% of the original volume to obtain 10 liters of concentrated solution, which is crystallized by the low-temperature crystallization method of a hot saturated solution, Its crystallization t...

Embodiment 2

[0026] A kind of preparation method of high-content epigallocatechin gallate according to the embodiment of the present invention 2 comprises the following steps:

[0027] Step 1: Take 2 kg of tea extract containing 75% epigallocatechin gallate, and dissolve the tea extract in 100 liters of 60°C pure water to obtain a tea polyphenol extract solution;

[0028] Step 2: adding the tea extract solution into the chromatographic column to control the adsorption flow rate;

[0029] Step 3: inject 20% ethanol into the chromatographic column after loading the column, and begin to collect the eluent, and the eluent collection volume is 6BV.

[0030] Step 4: Concentrate the collected eluent under reduced pressure or a single-effect concentrator under reduced pressure, and concentrate its volume to 2% of the original volume to obtain 8 liters of concentrated solution, which is crystallized by low-temperature crystallization of a hot saturated solution. Its crystallization temperature is ...

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Abstract

The invention relates to a preparation method of gallate with a high content of epigallocatechin. Powdery gallate with a high content of epigallocatechin is obtained by the steps of dissolving, loading to a column, eluting, concentrating, crystallizing, purifying and the like by taking tea polyphenol of gallate containing epigallocatechin as the raw material. The content of gallate of epigallocatechin is greater than or equal to 98%. In a production process, no toxic solvents are used, so that the production process is environmentally friendly, the solvent handling capacity is small, and the operation is simple and feasible. Ester catechins EGCG, GCG and ECG can be separated at one time, so that the gallate is good in effect, high in efficiency, high in content, short in crystallizing time, hard to oxidize, good in color and luster and high in purity.

Description

technical field [0001] The invention relates to the field of preparation of natural product catechin, in particular to a preparation method of high-content epigallocatechin gallate. Background technique [0002] Catechins include gallocatechin (GC), epicatechin gallate (ECG), epigallocatechin (EGC), epicatechin (EC), and epigallocatechin gallate (EGCG). Among them, EGCG is the main component of green tea catechins, and also the main component of green tea polyphenols. EGCG has a wide variety of biological activities, strong antioxidant activity, and can protect cells and DNA from damage. These effects of EGCG are mainly derived from its ability to scavenge oxygen free radicals, that is, its antioxidant capacity. EGCG plays an important role in the treatment of cancer and cardiovascular diseases. It can interfere with cancer cell signal transmission, inhibit the activity of carcinogens in the diet, and can be used as a reversal agent for tumor multidrug resistance, increasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 叶艳龙菲菲罗祥马松吴世华
Owner HUBEI ZHONGXIN BIOTECH CO LTD
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