A kind of rapid extraction method of blueberry pomace anthocyanin
A technology of blueberry pomace and extraction method, which is applied in the direction of chemical instruments and methods, organic chemistry, organic dyes, etc., can solve the problems of anthocyanin stability, unfavorable continuous production, and long extraction time, so as to shorten the production cycle, Less impurities and shorter extraction time
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Embodiment 1
[0017] Take 1 kg of frozen blueberry pomace, thaw it at room temperature, beat it into a homogenate with a homogenizer, and place it in an ultra-high pressure device for 5 minutes at 350 MPa and 40 °C. Add 5g of pectinase, 3g of hemicellulase, and 3g of cellulase to the pomace after ultra-high pressure treatment, and perform enzymatic hydrolysis at 50°C for 2h. Add 5L of ethanol-acetic acid-water (40:10:50, v / v / v) solution to the enzymatically hydrolyzed pomace, and process it for 7 minutes at 2000 rpm with a high-pressure homogenizer. The turbid liquid obtained after high-pressure homogenization is separated by centrifuge from pomace and anthocyanin extract, and the anthocyanin extract is passed through a vacuum rotary evaporator to remove ethanol and acetic acid at 45°C to obtain blueberry pomace Anthocyanin aqueous solution. The anthocyanin aqueous solution was placed in a vacuum freeze dryer to freeze-dry to obtain 4.8 g of blueberry pomace anthocyanin powder.
Embodiment 2
[0019] Take 10kg of frozen blueberry pomace, thaw it at room temperature, beat it into a homogenate with a homogenizer, and place it in an ultra-high pressure device for 7 minutes at 350MPa and 40°C. Add 50g of pectinase, 30g of hemicellulase, and 30g of cellulase to the pomace after ultra-high pressure treatment, and perform enzymatic hydrolysis at 50°C for 2h. Add 50L of ethanol-acetic acid-water (40:10:50, v / v / v) solution to the enzymatically hydrolyzed pomace, and use a high-pressure homogenizer to treat it at 2000 rpm for 7 minutes. The turbid liquid obtained after high-pressure homogenization is separated by centrifuge from pomace and anthocyanin extract, and the anthocyanin extract is passed through a vacuum rotary evaporator to remove ethanol and acetic acid at 45°C to obtain blueberry pomace Anthocyanin aqueous solution. The anthocyanin aqueous solution was placed in a vacuum freeze dryer to freeze-dry to obtain 49.5 g of blueberry pomace anthocyanin powder.
Embodiment 3
[0021] Take 20kg of frozen blueberry pomace, thaw it at room temperature, beat it into a homogenate with a homogenizer, and place it in an ultra-high pressure device for 7 minutes at 350MPa and 40°C. 100 g of pectinase, 60 g of hemicellulase, and 60 g of cellulase were added to the pomace after ultra-high pressure treatment, and hydrolyzed at 50° C. for 2 hours. Add 100L of ethanol-acetic acid-water (40:10:50, v / v / v) solution to the enzymatically hydrolyzed pomace, and use a high-pressure homogenizer to treat it at 2000 rpm for 7 minutes. The turbid liquid obtained after high-pressure homogenization is separated by centrifuge from pomace and anthocyanin extract, and the anthocyanin extract is passed through a vacuum rotary evaporator to remove ethanol and acetic acid at 45°C to obtain blueberry pomace Anthocyanin aqueous solution. The anthocyanin aqueous solution was placed in a vacuum freeze dryer to freeze-dry to obtain 99.3 g of blueberry pomace anthocyanin powder.
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