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A kind of Pichia burtonii and application thereof

A technology of Pichia pastoris and filamentous spores, which is applied in the fields of fermentation, fungi, microorganisms, etc., can solve the problems of little research and no reports, and achieves a non-environmental pollution, strong tolerance, easy cultivation and preservation and industrial production. Effect

Active Publication Date: 2019-11-22
GUIZHOU UNIV
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Problems solved by technology

[0004] At present, the strains used to produce arabitol are mainly Aspergillus, Debariae, Hansenula, etc., but there are few studies on the production of arabinitol by Pichia burtonii, and there is no relevant research. report

Method used

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Embodiment Construction

[0033] A method for separating Pichia burtonii FBKL2.0089 (Hyphopichia burtonii FBKL2.0089), comprising the following steps:

[0034] a. Isolation of strains: Weigh 10g of Daqu sample from the koji produced by Guizhou Center Brewing Group Co., Ltd., put it into a Erlenmeyer flask filled with 90ml of sterile water and glass beads, and shake for about 30min. Take 1ml of the sample suspension for 10-fold serial dilution by 10 -4 、10 -5 、10 -6 and 10 -7 Take 0.2ml of each diluted sample suspension and spread it on the separation medium, culture it statically at 28°C for 2 days, pick a single colony for further screening, and store it on the slant medium. b. Preservation and passage of strains:

[0035] b. Preservation and passage of strains:

[0036] 1. Strain preservation medium:

[0037] (1) Malt juice agar medium: malt extract powder 130g / L, chloramphenicol 0.1g / L, agar 15g / L, pH5.8-6.2, sterilized at 115°C for 20min.

[0038] (2) YEPD (YPD) medium: yeast extract 10g, pe...

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Abstract

The invention discloses a Pichia burtonii and its application. Its morphological characteristics are as follows: inoculate the strain on a PDA medium by streaking, culture at 28°C for 2 days, the diameter of the colony is 5-8 mm, and the colony is round and squid. The color is milky white, the texture is creamy, opaque, and the edges of the colonies are blanket-like. The invention can produce arabitol in Maotai-flavor liquor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Pichia burtonii FBKL2.0089 strain, and at the same time relates to the use of the Pichia burtonii FBKL2.0089 strain in producing arabitol. Background technique [0002] In the production of Maotai-flavored liquor, yeast is mainly divided into two categories from the function. One is cerevisiae; the other is adipogenic yeast. Studies have shown that in addition to the functions of producing wine and aroma, some yeasts also have the function of producing polyols, such as: arabitol, glycerol, xylitol, maltitol, etc., to increase the alcohol content of liquor. Sweetness improves the quality of liquor. [0003] With the change of people's consumption taste, the public has shifted from heavy-flavor type to aroma and taste. Therefore, the research on flavor substances in liquor is particularly important. Sweetness is a kind of taste that makes people feel more pleasant, esp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/18C12R1/84
CPCC12P7/18C12N1/165C12R2001/84
Inventor 王晓丹邱树毅
Owner GUIZHOU UNIV
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