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Detection and counting method for relative content of microorganism

A technology of relative content and counting method, applied in the field of microbial detection, can solve the problem of low accuracy, achieve the effect of detection accuracy and accuracy, improve comparability, and good data continuity

Active Publication Date: 2017-05-31
ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One reading can only record the positive reaction of the medium at a certain incubation time, and its accuracy is not high

Method used

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  • Detection and counting method for relative content of microorganism
  • Detection and counting method for relative content of microorganism
  • Detection and counting method for relative content of microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076]The samples in this example were collected above oil and gas traps in a certain sea area, and the hydrocarbon oxidizing bacteria were detected in marine sediments in order to study the development of hydrocarbon oxidizing bacteria above the oil and gas traps in the sea area.

[0077] The specific implementation steps of the method for detecting and counting the relative content of microorganisms of the present invention are as follows:

[0078] 1. According to the trap structure discovered by the geophysical method, sampling points were laid out in a uniform grid, and 42 samples were collected. The sampling interval was 500m, and the sampling depth was 20cm. The samples are quickly frozen after collection, kept frozen and transported to the testing laboratory, where they are stored in a frozen state.

[0079] 2. Weigh 5g of the sample and place it in 20mL of hydrocarbon oxidizing bacteria culture solution, mix well to make the sample stock solution. For the culture medi...

Embodiment 2

[0093] The samples in this example were collected above the oil and gas reservoirs confirmed by drilling in a certain sea area, and the hydrocarbon oxidizing bacteria in the marine sediments were taken to detect the hydrocarbon oxidizing bacteria in order to study the development of hydrocarbon oxidizing bacteria above the oil and gas traps in the sea area.

[0094] The specific implementation steps of the method for detecting and counting the relative content of microorganisms of the present invention are as follows:

[0095] 1. For oil and gas reservoirs confirmed by drilling in a certain sea area, sampling points were arranged in the form of survey lines, and 23 samples were collected, with a sampling interval of 500m and a sampling depth of 20cm. The samples are quickly frozen after collection, kept frozen and transported to the testing laboratory, where they are stored in a frozen state.

[0096] 2. Weigh 5g of the sample and place it in 20mL of hydrocarbon oxidizing bact...

Embodiment 3

[0107] 1. Activate the Acinetobacter isolated from the ocean. The activation steps are as follows: first, separate the strains on the solid culture of marine hydrocarbon-oxidizing bacteria, and then pick a single colony and inoculate it on the liquid culture medium of marine hydrocarbon-oxidizing bacteria. Enrichment culture.

[0108] 2. Dilute the enriched and cultured Acinetobacter with sterile water to prepare a series of 15 different concentrations of Acinetobacter dilutions, and use a hemocytometer under a microscope to count the number of cells in this series of different concentrations of bacteria. The concentration of this series of 15 Acinetobacter dilutions with different concentrations can be obtained, expressed in units / mL.

[0109] 3. Pipette 1mL of the Acinetobacter solution in step 2 into 9mL of sterilized culture solution, and mix well to make a small dilution culture solution. Then take 1mL from the small dilution culture solution into another 9mL sterilized ...

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Abstract

The invention relates to the technical field of microorganism detection, and particularly relates to a detection and counting method for the relative content of a microorganism. The method comprises the following steps: adding an oxidation-reduction indicator into a culture medium to obtain an indicator culture medium, wherein the color change interval of the oxidation-reduction indicator comprises three or more colors which can be easily recognized by naked eyes; diluting a sample to be detected, and culturing the diluted sample to be detected through the indicator culture medium, wherein multiple dilutions are used for the diluting operation, and multiple parallel samples are used for each dilution; reading the color or absorbency of the indicator culture medium during and / or the culturing operation; and obtaining the relative content of a microorganism in the sample to be detected according to the color change degree or absorbency change value of the indicator culture medium. Compared with the conventional MPN (most probable number) method, the method provided by the invention detects the relative contents of microorganisms in different samples more precisely and accurately; the comparability of the relative contents of microorganisms in different samples is higher; and the obtained data have favorable continuity. Thus, the method is wide in application range.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a detection and counting method for the relative content of microorganisms. Background technique [0002] Driven by the reservoir pressure, the light hydrocarbon gas in the oil and gas reservoir migrates vertically upward along complex microfractures in the form of microbubble floating or continuous gas phase flow. During the migration of light hydrocarbons into the surface sediments, some of them become the food (carbon source) of obligate hydrocarbon-oxidizing bacteria in the soil, resulting in the abnormal development of hydrocarbon-oxidizing bacteria; the other part is absorbed by clay minerals and secondary carbonate cements. pack. Therefore, microbial anomalies and adsorbed hydrocarbon anomalies that are positively related to the abundance and pressure of the underlying oil and gas reservoirs are formed in the surface soil above the reservoirs. Using micro...

Claims

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Application Information

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IPC IPC(8): C12Q1/64C12Q1/06G01N21/31
CPCG01N21/31C12Q1/06C12Q1/64C12Q2304/22G01N31/22G01N21/78
Inventor 郝纯梅海
Owner ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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