Recycling elution device for detecting estrogen in environmental water body and application of recycling elution device
A technology for eluting devices and estrogen, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, and can solve problems such as secondary pollution, time-consuming detection, organic solvents, and complexity
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Embodiment 1
[0037] Such as Figure 1-3 Shown, the present invention a kind of circulating eluting device of estrogen detection in the environmental water body, comprises: iron frame platform 1, liquid storage bottle 2, filtration type purification column 4, elution bottle 5, high performance liquid chromatography / ultraviolet detector (HPLC / UV) 6 etc.
[0038] A liquid storage bottle 2 is installed on the iron stand 1 , and the liquid storage bottle 2 contains a sample liquid 3 . One end of the filter-type purification column 4 goes deep into the liquid storage bottle 2 through a pipeline, and the other end of the filter-type purification column 4 is connected to a high-performance liquid chromatography / ultraviolet detector (HPLC / UV) 6 through a pipeline. A peristaltic pump 7 is connected to the side of the high performance liquid chromatography / ultraviolet detector (HPLC / UV) 6 . One end of the elution bottle 5 goes deep into the liquid storage bottle 2 through a pipeline, and the other ...
Embodiment 2
[0046] β-cyclodextrin functionalization
[0047] 0.8g of β-cyclodextrin was dissolved in 30ml of anhydrous dimethylformamide (DMF), 0.8g of NaH was added, and magnetically stirred at 800rpm for 1 hour. Excess NaH was removed by filtration. 2 ml of γ-(methacryloxy)propyltrimethoxysilane (GPTMS) was added dropwise under magnetic stirring. The reaction was carried out at 80° C. for 12 hours. After the reaction, the solution became viscous. The precipitate was washed successively with anhydrous DMF, methanol and acetone. Dry in vacuum at 110°C for 12 hours. Store in a desiccator until use.
Embodiment 3
[0049] Synthesis of Fe3O4@TiO2-MIPs Magnetic Microspheres by Surface Initiated RAFT
[0050] 1) Dissolve 1.0 mmol of bisphenol A (template molecule), 2.0 mmol of 4-vinylpyridine (functional monomer), and 2.0 mmol of alkylated cyclodextrin in 10 ml of DMSO, mix well, and store in the dark for 12 hours. In this mixture, add ferric oxide microspheres (200mg), crosslinking agent (TRIM, polyglycerol trimethacrylate 20.0mmol), initiator (AIBN, 60mg), dispersion medium (water, 80ml) , and stir evenly for 30 minutes.
[0051] 2) The obtained mixed solution was transferred to a 300ml flask, and nitrogen gas was continuously fed in during the reaction, and the reaction was carried out at 70° C. for 24 hours. After the reaction is complete, the product is magnetically separated.
[0052] 3) Soxhlet extraction with 20% acetic acid methanol solution to obtain a gray powder that removes BPA, and vacuum-dry the resulting product to obtain the magnetic bisphenol A imprinted polymer Fe 3 ...
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