Method for quickly detecting aflatoxin M1

A kind of aflatoxin, rapid technology, applied in the field of detection, can solve the problems of expensive, complicated processing, time-consuming, etc., and achieve the effect of shortening the detection time, high sensitivity and specificity

Pending Publication Date: 2017-05-31
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chromatography has the characteristics of high sensitivity and good detection results, but the sample pretreatment process is complicated, time-consuming, and requires expensive large-scale instruments, which is difficult to meet the needs of rapid on-site detection.
Immunoassay is a commonly used detection method, which is fast and sensitive, but requires the use of expensive and easily inactivated enzymes, antibodies and other biological reagents

Method used

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  • Method for quickly detecting aflatoxin M1
  • Method for quickly detecting aflatoxin M1
  • Method for quickly detecting aflatoxin M1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Rapid detection method of aflatoxin M1

[0023] Measure the fluorescence intensity of 10ng / mL aflatoxin M1 with a fluorescence spectrophotometer (such as figure 1 shown), the excitation wavelengths are 365nm and 399nm. It can be seen from the figure that aflatoxin M1 has no peak at 619nm. After mixing 0.01-2ng / mL aflatoxin M1 standard solution with 2μM porphyrin fluorescent dye NMM, the fluorescence intensity was measured with a fluorescence spectrophotometer. The excitation wavelength is 399nm and the emission wavelength is 619nm. Taking the concentration of aflatoxin M1 as the abscissa, with (F-F 0 ) / (F'-F 0 ) is a fitting curve for the ordinate, where F 0 , F and F'represent the fluorescence intensity of NMM without aflatoxin M1, the fluorescence intensity of NMM after adding different concentrations of aflatoxin M1 and the fluorescence intensity of NMM when adding 2ng / mL aflatoxin M1 (such as figure 2 shown).

Embodiment 2

[0025] Aflatoxin B1 (AFB1), phyllotoxin (PAT), zearalenone (ZEN), ochratoxin A (OTA) and T-2 toxin were selected as competitors to study the specificity of the method. Prepare aflatoxin M1 at a concentration of 1 ng / mL and a competitor toxin at a concentration of 10 ng / mL. Mix with porphyrin dye NMM respectively, and measure its fluorescence value with a fluorescence spectrophotometer. Such as image 3 As shown, aflatoxin M1 can strongly increase the fluorescence of NMM. Competing toxins have little effect on their fluorescence. It shows that the method is specific to aflatoxin M1.

Embodiment 3

[0027] Aflatoxin B1 (AFB1), phyllotoxin (PAT), zearalenone (ZEN), ochratoxin A (OTA) and T-2 toxin were selected as competitors, and PPIX was used as a non-fluorescence-labeled porphyrin The specificity of the method when dyes. Prepare aflatoxin M1 at a concentration of 1 ng / mL and a competitor toxin at a concentration of 10 ng / mL. Mix with porphyrin dye PPIX respectively, and measure its fluorescence value with a fluorescence spectrophotometer. The excitation wavelength is 410nm and the emission wavelength is 624nm. Such as Figure 4 As shown, aflatoxin M1 can strongly increase the fluorescence of PPIX. Competing toxins have little effect on their fluorescence. It shows that the method is specific to aflatoxin M1.

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Abstract

The invention discloses a novel method for quickly detecting aflatoxin M1. The method is used for detecting aflatoxin M1 mainly on the basis of non-labeled porphyrin fluorescent dyes. According to the method, after aflatoxin M1 and porphyrin fluorescent dyes are mixed, the fluorescence intensity of the dyes increases, and the fluorescence intensity is detected to implement quick and quantitative detection on the aflatoxin M1. The method disclosed by the invention is simple, has the advantages of high specificity and high detection speed, and can satisfy the quick and accurate field detection requirements.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a rapid detection method for detecting aflatoxin M1 based on a non-labeled porphyrin dye. Background technique [0002] Aflatoxin is a derivative of dihydrofuranocoumarin, a secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus, which has acute toxicity, chronic toxicity and carcinogenicity. Aflatoxins often exist in foods such as corn, peanuts, and grains, and are classified as Class 1 carcinogens by the World Health Organization. Countries around the world have set strict limits for aflatoxins. Milk and dairy products are one of the foods that are susceptible to aflatoxin contamination. When dairy cows ingest feed contaminated with aflatoxin, aflatoxin can be hydroxylated in the body to produce aflatoxin M1 (Aflatoxin M1, AFM1), and the milk produced contains AFM1. After people eat the milk, it will cause serious harm to human health. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 刘亚青王硕郭婷林晓东高金婷邓健康
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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