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Detection method for residual apramycin in chicken and test strip

A technology of apramycin and detection method, which is applied in the field of chemical experiments, can solve the problems of complicated instrument analysis operation, high cost of detection equipment, soft animal body, etc., and achieve the effect of reducing cost, short detection time and reducing loss

Inactive Publication Date: 2017-05-31
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] According to Chinese Patent No. CN101718788A, a method for detecting apramycin and the detection method proposed in its special mold-linked immunoassay kit, the general instrument analysis operation is more complicated, and the sensitivity is relatively low, the detection time is relatively long, and it is inconvenient to use , and the cost of large-scale testing equipment is relatively high
[0004] However, if apramycin is used too much, it will cause the animal body to become weak, so the dosage should be controlled when using it, and if there is residual apramycin in the chicken, it will cause great harm. Therefore, the present invention aims to Proposing a detection method for detecting apramycin residues in chicken and its test strip

Method used

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  • Detection method for residual apramycin in chicken and test strip

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Embodiment 1

[0022] A method for detecting residues of apramycin in chicken, comprising the following steps:

[0023] S1. First, buy a live chicken with normal size and healthy growth from home, farm and vegetable market respectively. By selecting samples in different environments, you can know whether the residue of apramycin is from the test results. It is related to the growth environment of the animal, then kill the chicken and take out 1-2 kg of chicken tissue respectively. After taking out the chicken tissue, remove the bones in the chicken, then chop the chicken into minced meat and fully stir it, and take out the chicken The tissues were placed in different volumetric flasks and marked A1, A2, and A3 respectively, and the apramycin solution was taken out as a contrast, and A4 was marked at the same time, and compared with the reaction phenomenon of the apramycin solution contrast, It can be clearly seen whether the chicken contains apramycin;

[0024] S2. Take out 2 grams of chick...

Embodiment 2

[0029] A method for detecting residues of apramycin in chicken, comprising the following steps:

[0030] S1. First, buy a live chicken with normal size and healthy growth from home, a farm and a vegetable market respectively, then kill the chicken and take out 1-2 kg of chicken tissue. After taking out the chicken tissue, remove the chicken Then the chicken was chopped into minced meat and fully stirred, the chicken tissues taken out were placed in different volumetric flasks and marked A1, A2 and A3 respectively, and the apramycin solution was taken out as a contrast, At the same time mark A4;

[0031] S2. Take out 3 grams of chicken tissue from A1, A2 and A3 respectively, then put the taken out chicken tissue into a polystyrene centrifuge tube, add phosphate buffer and trichloroacetic acid, shake evenly for 8 minutes, and put the selected The chicken tissue is chopped, and then the machine is used to make minced meat, and fully stirred, and then the chicken is cooked withou...

Embodiment 3

[0036] A method for detecting residues of apramycin in chicken, comprising the following steps:

[0037] S1. First, buy a live chicken with normal body size and healthy growth from home, farm and vegetable market respectively, then kill the chicken and take out 1-2 kg of chicken tissue. After taking out the chicken tissue, remove the chicken meat Then the chicken was minced into minced meat and fully stirred, the chicken tissues taken out were placed in different volumetric flasks and marked A1, A2 and A3 respectively, and the apramycin solution was taken out as a contrast, At the same time mark A4;

[0038] S2. Take out 1.5 grams of chicken tissue from A1, A2 and A3 respectively, put the chicken tissue into the machine to make mince, then put the mince into a polystyrene centrifuge tube, and add phosphate buffer and trichloro Acetic acid, shake evenly for 5 minutes, filter the mixture, and take out the filtrate;

[0039] S3, 2.5 milliliters of the obtained filtrate is taken...

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Abstract

The invention discloses a detection method for residual apramycin in chicken and a test strip. The detection method comprises the following steps: S1, first buying a live chicken with a normal body shape and a healthy growth condition separately from an individual farmer, a livestock farm and a vegetable market, then killing the chickens, taking out 1 to 2 kg of chicken tissues, placing the fetched chicken tissues respectively in different volumetric flasks, respectively marking as A1, A2 and A3, taking out an apramycin solution as a reference, marking as A4 and S2, and respectively taking out 1g to 5g of chicken tissues from A1, A2 and A3. The detection method adopted by the invention is simple in operation, short in detection time and capable of performing the detection at any time, and can judge reasons causing the residual apramycin in the chicken and an amount of the residual apramycin in the chicken to improve a raising method, so that chicken become healthier, the harm to a human body can be avoided, the loss can also be reduced, and the cost can be decreased.

Description

technical field [0001] The invention relates to the technical field of chemical experiments, in particular to a method for detecting residues of apramycin in chicken and a test strip thereof. Background technique [0002] Apramycin, also known as apramycin, is a new aminoglycoside veterinary antibiotic produced by Streptococcus obliquus. It was successfully developed by the United States in the 1980s. It is characterized by a wide antibacterial spectrum and is not easy to produce drug resistance. It can be used Infection caused by Escherichia coli, Salmonella and mycoplasma in chicken. As a drug-type feed additive, apramycin can significantly promote weight gain and improve feed conversion rate, and has been widely used in the field of livestock and poultry breeding. [0003] According to Chinese Patent No. CN101718788A, a method for detecting apramycin and the detection method proposed in its special mold-linked immunoassay kit, the general instrument analysis operation is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/532G01N33/543
CPCG01N33/543G01N33/532G01N2333/36
Inventor 周合张根义张进周朱晨胡彬吴念绮杨敏
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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