Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for quickly identifying autoimmune urticaria sample in biological sample bank

An autoimmune, biological sample technology, applied in the field of rapid identification of autoimmune urticaria samples in biological sample banks, can solve problems such as difficult identification of samples, and achieve the effect of rapid identification and convenient management

Inactive Publication Date: 2017-05-31
PEKING UNIV SHENZHEN HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no rapid detection method for autoimmune urticaria samples, therefore, it is difficult to identify samples in biobanks accurately and quickly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly identifying autoimmune urticaria sample in biological sample bank
  • Method for quickly identifying autoimmune urticaria sample in biological sample bank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The embodiment of the present invention provides a method for rapidly identifying autoimmune urticaria samples in a biological sample bank, comprising the following steps:

[0027] 1) Using enzyme-linked immunosorbent assay or colloidal gold method to make the IgE receptor protein react with the anti-IgE receptor protein antibody in the serum sample.

[0028] 2) Detecting the content of anti-IgE receptor protein antibody in the serum sample, when the anti-IgE receptor protein antibody exceeds a predetermined threshold, the blood is an autoimmune urticaria sample.

[0029] According to the method for rapidly identifying autoimmune urticaria samples in the biological sample bank provided by the embodiment of the present invention, the autoimmune urticaria samples can be quickly identified, and the management in the biological sample bank is convenient.

Embodiment 2

[0031] The embodiment of the present invention provides a method for rapidly identifying autoimmune urticaria samples in a biological sample bank. The method takes the enzyme-linked immunosorbent assay method as an example to illustrate the present invention, which includes the following steps:

[0032] 1) Coating the IgE receptor protein on a solid phase carrier.

[0033] Specifically, the coating buffer can be used to dilute the IgE receptor protein to a solubility of 1-10 μg / m, and the diluted IgE receptor protein can be added to the solid phase carrier, and then incubated for 1 to 3 hours to form a solid phase carrier. Phase IgE receptor protein (solid-phase antigen), that is, allow IgE receptor protein to bind to the surface of the solid-phase carrier. Preferably, the incubation temperature is 35-38°C, more preferably, the incubation temperature is 37°C.

[0034] It can be understood that the solid phase carrier can be a test tube, beads or a microwell plate made of poly...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for quickly identifying an autoimmune urticaria sample in a biological sample bank. The method comprises the following steps of making IgE (Immunoglobulin E) receptor protein and an IgE receptor protein antibody in a serum sample generate a reaction by using an enzyme-linked immunosorbent assay method or a colloidal gold method; detecting the content of the IgE receptor protein antibody in the serum sample, wherein the blood is the autoimmune urticaria sample when the IgE receptor protein antibody exceeds a predetermined threshold value. According to the method for quickly identifying the autoimmune urticaria sample in the biological sample bank, which is provided by an embodiment of the invention, the autoimmune urticaria sample can be quickly identified; the management in the biological sample bank is convenient.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for rapidly identifying autoimmune urticaria samples in a biological sample bank. Background technique [0002] Biobank mainly refers to the standardized collection, processing, storage and application of biological macromolecules, cells, tissues and organs and other samples of healthy and diseased organisms (including human organ tissues, whole blood, plasma, serum, biological fluids or processed The biological samples (DNA, RNA, protein, etc.) and the clinical, pathological, treatment, follow-up, informed consent and other data related to these biological samples and their quality control, information management and application systems. However, in the biological sample bank, often These samples need to be tested to identify which type of disease these samples belong to. [0003] Autoimmune urticaria refers to urticaria caused by functional autoantibodies cr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6881
Inventor 王庆文方征宇韦伟曾杏珍杨旸李粉玲刘雅菁李玉霞曾沛英
Owner PEKING UNIV SHENZHEN HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com