Quantitative detection kit for growth hormone and preparation method of quantitative detection kit

Abstract

The invention discloses a quantitative detection kit for growth hormone. The quantitative detection kit comprises an experimental buffer solution, concentrated washing liquor, an enhancement solution, a coated plate, a standard product, a quality control product and a marker, wherein the enhancement solution is prepared from 1 to 5 mg / L of beta-naphthoyltrifluoroacetone, 15 to 30 mg / L of tri-n-octylphosphine oxide and a third buffer solution; the coated plate is prepared from coating liquid, confining liquid and a reaction plate; the coating liquid is prepared from 1 to 10 ppm of Proclin300, a fourth buffer solution and 1 to 10 [mu]g / ml of a growth hormone monoclonal antibody; the confining liquid is prepared from 1.0 to 10.0 g / L of trehalose, bovine serum albumin with the mass volume ratio of 0.4 to 0.6 percent and the fourth buffer solution; the standard quality and the quality control product are filter paper dried blood pieces; the filter paper dried blood pieces comprise mixtures with 8 concentrations; the mixtures comprise red blood cells, de-hormone negative serum mixed matrixes and the growth hormone; and the marker is prepared from the growth hormone monoclonal antibody and a lanthanide ion chelate according to (1: 1) to (1: 6) m / m. The invention further discloses a preparation method of the kit. The kit disclosed by the invention can ensure that a detection result is highly consistent with a detection result of a serum sample.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a growth hormone quantitative detection kit and a preparation method thereof. Background technique [0002] Growth Hormone (GH) is a protein secreted by pituitary gland cells. It is a peptide hormone. The determination of serum growth hormone is helpful for the diagnosis of gigantism, acromegaly, and growth hormone deficiency. At present, more than 90% of the growth hormone concentration detection kits use the chemiluminescence method, and all of them use serum samples as the detection objects. For the detection of serum samples, it has the following disadvantages: high cost, difficult preservation of blood samples, inconvenient long-distance delivery, and potential biological safety hazards during transportation, which brings inconvenience to the tester; The patient takes drugs for growth hormone stimulation test, and draws blood multiple times at different time periods, usually mo...

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention is to provide a growth hormone quantitative detection kit and its preparation method, which can use time-resolved immunofluorescence assay technology to carry out quantitative detection of growth hormone, and during detection, dry blood slices on filter paper are used as detection samples, which can Avoid the shortcomings of serum samples that are not easy to store, inconvenient to transport, require growth hormone stimulation test, and large blood collection volume, and at the same time ensure that the test results are highly consistent with those of serum samples

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