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Polymer tissue engineering scaffold porous material having good pore connectivity and preparation method thereof

A technology of pore connectivity and tissue engineering, applied in medical science, prosthesis, etc., can solve the problems of poor connectivity, limited cell culture, etc., and achieve the effects of low cost, good connectivity, and avoidance of thermal degradation.

Active Publication Date: 2017-06-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although porous scaffolds with appropriate pore sizes can be prepared by this method, the porous scaffolds produced by this method have poor connectivity between pores, which limits cell culture in tissue engineering.

Method used

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  • Polymer tissue engineering scaffold porous material having good pore connectivity and preparation method thereof
  • Polymer tissue engineering scaffold porous material having good pore connectivity and preparation method thereof
  • Polymer tissue engineering scaffold porous material having good pore connectivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Weigh two kinds of polymers according to the volume ratio of polylactic acid (PLA): polyvinylpyrrolidone (PVP) = 50: 50, premix the two kinds of polymers at room temperature to obtain a premix, and then premix the The mixture was mixed in a micro extruder at 180°C under normal pressure for 8 minutes at a speed of 80rpm to obtain a melt blend of the two polymers;

[0027] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLA / PVP flake blend green body. The molding temperature is 180° C., and the molding pressure is 10 MPa , molding time is 5min;

[0028] (3) Place the flake-shaped blended body obtained in step (2) in a high-pressure reactor, feed foaming gas carbon dioxide and raise the temperature to 150°C and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into a supercritical fluid ) heat preservation and pressure for 4 hours to coarsen the flake blended body, and ma...

Embodiment 2

[0031] (1) Weigh two kinds of polymers according to the volume ratio of polylactic acid (PLA):polycaprolactone (PCL)=50:50, and pre-mix the two kinds of polymers at room temperature to obtain a premix, and then premix The mixture was mixed for 5 minutes at 50 rpm in a micro extruder at normal pressure and 180° C. to obtain a melt blend of the two polymers;

[0032] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLA / PCL flake blend body. The molding temperature is 180° C., and the molding pressure is 10 MPa , molding time is 3min;

[0033](3) Place the sheet-like blend green body obtained in step (2) in a high-pressure reactor, introduce foaming gas carbon dioxide and raise the temperature to 120°C and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into supercritical Fluid) heat preservation and pressure for 1h to coarsen the flake blend body, then heat preservation and pressu...

Embodiment 3

[0036] (1) Weigh the two polymers according to the volume ratio of polylactic-co-glycolic acid (PLGA): polyethylene glycol (PEG) = 60: 40, and pre-mix the two polymers at room temperature to obtain a pre-mixed Then the premix was mixed at normal pressure and 140°C in a micro extruder at a speed of 80rpm for 8min to obtain a melt blend of the two polymers;

[0037] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLGA / PEG flake blend green body. The molding temperature is 140° C., and the molding pressure is 10 MPa , molding time is 3min;

[0038] (3) Place the sheet-like blend green body obtained in step (2) in a high-pressure reactor, introduce foaming gas carbon dioxide, heat up to 100°C, and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into supercritical Fluid) heat preservation and pressure for 2 hours to coarsen the flake blend body, then heat preservation and pressure f...

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Abstract

A preparation method of a polymer tissue engineering porous scaffold material having good pore connectivity includes the steps: (1) melting and blending two kinds of thermoplastic polymers to obtain a molten blend material; (2) pressing the molten blend material into a blend blank body; (3) placing the blend blank body in a high pressure reaction kettle, introducing foaming gas, heating to 50-110 DEG C, pressurizing to 5-25 MPa, carrying out heat and pressure preservation for 0.5-4 h for coarsening the blend blank body, then at the temperature of 40-150 DEG C and the pressure of 8-25 MPa, making supercritical fluid in the high pressure reaction kettle reach a saturated state in the blend blank body, then keeping the temperature unchanged and reducing the pressure in the high pressure reaction kettle to ordinary pressure at a rate of 5-15 MPa / s, making the blend blank body foamed, and carrying out cooling setting, to obtain a foamed blank body; and (4) dipping the foamed blank body in an etching solution, and etching. The method can further improve the connectivity between pores of the polymer tissue engineering scaffold material.

Description

technical field [0001] The invention belongs to the fields of foaming polymer material processing and biomedicine, in particular to a polymer tissue engineering porous support material and a preparation method thereof. Background technique [0002] Tissue engineering is an emerging discipline that has emerged in recent years. It cultivates normal tissue cells in vitro and attaches them to a scaffold material with good cytocompatibility and biodegradability to form a cell-scaffold complex. The complex is implanted into the body tissue or the damaged part of the organ, and a new tissue structure is gradually formed in the cell body, while the scaffold material is gradually degraded and absorbed by the organism, so as to achieve the purpose of repairing and rebuilding the tissue body. There are three core elements in tissue engineering: seed cells, tissue engineering scaffold materials, and growth factors. Scaffold materials play an important role in tissue engineering, becaus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/56
CPCA61L27/18A61L27/56C08L67/04
Inventor 廖霞叶建刚黄二波李光宪
Owner SICHUAN UNIV