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Ultrafiltration/nanofiltration double-membrane high-efficiency pumpkin oligosaccharide purifying method

A technology of oligosaccharides and ultrafiltration membranes, applied in ultrafiltration, chemical instruments and methods, membranes, etc., can solve the problems that pumpkin oligosaccharides have not been reported, and achieve compact production process, high-efficiency removal, and mild treatment conditions Effect

Inactive Publication Date: 2017-06-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Purification of pumpkin oligosaccharides by ultrafiltration / nanofiltration double-membrane technology has not been reported yet

Method used

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  • Ultrafiltration/nanofiltration double-membrane high-efficiency pumpkin oligosaccharide purifying method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for ultrafiltration / nanofiltration double-membrane efficient purification of pumpkin oligosaccharides is carried out according to the following steps:

[0023] (1) Remove the seeds of the pumpkin and cut into pieces, add 3 times the weight of water to make a slurry, use pectinase to enzymatically hydrolyze, take the supernatant by centrifugation, concentrate to 1 / 3 of the original supernatant volume, add 3 times the volume of 80% ethanol Precipitate, centrifuge and take the supernatant to obtain the pumpkin oligosaccharide crude extract;

[0024] (2) Pass the pumpkin oligosaccharide crude extract through a 20 μm filter bag and dilute to a conductivity of 4411ppm, and the temperature of the feed liquid is 21°C;

[0025] (3) Perform ultrafiltration, the working frequency of the ultrafiltration section is 26.7Hz, the inlet pressure is 0.6MPa, and the permeate flow rate is 16L / h;

[0026] (4) When the stock solution of the feed liquid is concentrated to 1 / 3 of the...

Embodiment 2

[0032] A method for ultrafiltration / nanofiltration double-membrane efficient purification of pumpkin oligosaccharides is carried out according to the following steps:

[0033] (1) Remove the seeds of the pumpkin and cut into pieces, add 2 times the weight of water to make a slurry, use amylase to enzymatically hydrolyze, take the supernatant by centrifugation, concentrate to 1 / 2 of the original supernatant volume, add 2 times the volume of 80% ethanol to precipitate , and centrifuge to take the supernatant to obtain the pumpkin oligosaccharide crude extract;

[0034] (2) Pass the pumpkin oligosaccharide crude extract through a 20 μm filter bag and dilute to a conductivity of 4233ppm, and the temperature of the feed liquid is 23°C;

[0035] (3) The working frequency of the ultrafiltration section is 27.1Hz, the inlet pressure is 0.6MPa, and the permeate flow rate is 16L / h;

[0036] (4) When the stock solution of the feed liquid is concentrated to 1 / 4 of the volume of the feed ...

Embodiment 3

[0042] A method for ultrafiltration / nanofiltration double-membrane efficient purification of pumpkin oligosaccharides is carried out according to the following steps:

[0043](1) Remove the seeds and cut the pumpkin into pieces, add 5 times the weight of water to make a slurry, enzymatically hydrolyze with pullulanase, centrifuge to get the supernatant, concentrate to 1 / 5 of the original supernatant volume, add 4 times the volume of 80 % ethanol precipitation, centrifugation to take the supernatant to obtain the pumpkin oligosaccharide crude extract

[0044] (2) Pass the pumpkin oligosaccharide crude extract through a 20 μm filter bag and dilute to a conductivity of 4052ppm, and the temperature of the feed liquid is 19°C;

[0045] (3) The working frequency of the ultrafiltration section is 27.6Hz, the inlet pressure is 0.7MPa, and the permeate flow rate is 18L / h;

[0046] (4) When the stock solution of the feed liquid is concentrated to 1 / 2 of the volume of the feed liquid af...

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Abstract

The invention discloses an ultrafiltration / nanofiltration double-membrane high-efficiency pumpkin oligosaccharide purifying method. According to the method, an ultrafiltration technology and a nanofiltration technology are applied to a pumpkin oligosaccharide production technology, so that the purpose of purifying pumpkin oligosaccharide in high efficiency is achieved. Macromolecules of protein, polysaccharide and the like are intercepted by an ultrafiltration membrane, and a nanofiltration membrane penetrates through micromolecules of inorganic salt, monosaccharide and the like to achieve a concentrating effect. The method disclosed by the invention achieves separation, refining and concentration of the pumpkin oligosaccharide through the utlrafiltration technology and the nanofiltration technology, has a compact production technology, is convenient to operate and has moderate processing conditions, high preparing efficiency, low energy consumption and a good oligosaccharide separation effect. An ultrafiltration and nanofiltration double-membrane technology is an ideal pumpkin oligosaccharide purifying method, can remove macromolecular impurities and micromolecular impurities in high efficiency and improves concentration and quality of a pumpkin oligosaccharide product.

Description

technical field [0001] The invention relates to a method for extracting pumpkin oligosaccharides, in particular to a method for efficiently purifying pumpkin oligosaccharides with ultrafiltration / nanofiltration double membranes. Background technique [0002] Oligosaccharides (oligosaccharides) can also be called oligosaccharides, which are low-degree polymerized sugars connected by 2-10 monosaccharides through glycosidic bonds to form straight or branched chains, with a molecular weight between 200-300D. Due to the different binding positions and types of monosaccharide molecules, a rich variety of oligosaccharides is formed. Oligosaccharides have good functional properties and can be used as a source of sweetness, but they are not degraded by human gastric acid and gastric enzymes, and are not absorbed in the small intestine. After entering the large intestine, they selectively promote the proliferation of beneficial bacteria and inhibit pathogenic bacteria and diarrhea. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D61/58B01D61/14B01D61/02B01D65/06
CPCB01D61/027B01D61/145B01D61/58B01D65/06B01D2311/2649B01D2321/164
Inventor 李全宏梁莉
Owner CHINA AGRI UNIV
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