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A microfluidic chip-based method for typing serum glucose profiles

A microfluidic chip and typing method technology, applied in the field of sugar analysis, can solve problems such as long-term separation mode, and achieve the effects of rapid separation and analysis capability, efficient separation and detection, and high resolution

Active Publication Date: 2019-07-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still requires a long time and different separation modes for glycotyping.

Method used

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  • A microfluidic chip-based method for typing serum glucose profiles
  • A microfluidic chip-based method for typing serum glucose profiles
  • A microfluidic chip-based method for typing serum glucose profiles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Selection of electrophoresis buffer system:

[0019] The injection buffer system consists of 50mmol / L tris(Tris), 50mmol / L N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 1mmmol / L ethylenediaminetetra Acetic acid (EDTA) is composed of 1×TTE buffer solution, pH8.3; the sample separation system is composed of POP 4 or POP 7 gel;

[0020] 2) Preparation and labeling of serum samples

[0021] Take 100μL of blood, centrifuge at 3000r / min for 10min to separate the serum, add 2μL of 10mmol / lNH to the 2μL serum sample 4 HCO 3 Buffer and 3 μL deionized water, heat at 95°C for 5 minutes, put in the refrigerator (4°C) for about 15 minutes, add N-glycopeptidase F, incubate in a 37°C incubator for 12 hours (or overnight), and put it in the refrigerator (4°C) ) in an ice bath for 15 minutes, centrifuged for 15 minutes, and the supernatant after centrifugation was used for determination; take 6 μL of centrifuged and dried at 60°C for 120 minutes without a cover (...

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Abstract

The invention discloses a microfluidic chip-based method for typing blood sugar profiles, and relates to the field of sugar analysis. The method includes the steps of selecting a buffer system, selecting an electrophoresis chip, serum samples and markers, electrophoresis and the like. Based on the chip electrophoresis technology combined with the laser-induced fluorescence detection system, the present invention separates 12 N-glycan chains appearing in the serum within 8 minutes, and makes up for the deficiency that the conventional blood biochemical analysis method cannot analyze the N-glycan spectrum. The high-resolution and rapid separation and analysis capabilities of chip electrophoresis for serum N-glycan profiles make it promising to become one of the analytical methods for fast, simple and efficient separation and detection of clinical serum N-glycan profiles.

Description

technical field [0001] The invention relates to the field of sugar analysis, in particular to a microfluidic chip-based serum sugar profile typing method. Background technique [0002] All cells and most macromolecules in nature are covered with sugars. Sugars are closely related to life activities. They play an important role of "recognition marks" in the recognition, adhesion and signal transduction between cells, cells and matrix or foreign objects. In recent years, studies have found that there are often changes in sugar chains on the surface of malignant tumor cells, and the relationship between sugar chain structure and diseases, especially tumors, has attracted more and more attention. [0003] At present, reports at home and abroad have analyzed the sugar chain structure from multiple aspects. The main methods are: sugar micro-dot matrix, fluorescent sugar-binding probe, nuclear magnetic resonance, high performance liquid chromatography, time-of-flight mass spectrom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N21/64
Inventor 秦建华苏文涛姜雷李艳峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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