A device for treating sewage
A sewage and bioreactor technology, applied in the direction of biological treatment devices, sedimentation treatment, multi-stage water treatment, etc., can solve the problems of high input cost and unsuitable for large-scale promotion and use
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Embodiment 1
[0018] The preparation of embodiment 1 transgenic engineering bacteria
[0019] The denitrifying phosphorus accumulating bacterium Bacillus cereus H-hrbOl with the preservation number of CGMCC No. 5939 is named H-hrbOl-1 by introducing the enzyme described in SEQ ID NO: 1 through a conventional test method in the field.
[0020] Further, variants of the enzyme are provided, respectively, on the basis of SEQ ID NO: 1, there is one in L60T, L70F, A121D, G132N, L181S, Y194V, P205A, D241Q, D259R, R262Y, G279T, or V299F mutation. The strains into which the mutated enzymes were introduced were named: H-hrbOl-1-L60T, H-hrbOl-1-L70F, H-hrbOl-1-A121D, H-hrbOl-1-G132N, H-hrbOl-1- L181S, H-hrbOl-1-Y194V, H-hrbOl-1-P205A, H-hrbOl-1-D241Q, H-hrbOl-1-D259R, H-hrbOl-1-R262Y, H-hrbOl-1-G279T, H-hrbOl-1-V299F.
Embodiment 2
[0021] The preparation of embodiment 2 immobilized microspheres
[0022] Preparation of immobilized blank pellets: drop 2.5% chitosan colloidal solution into the coagulation solution (20% methanol and 30% NaOH) with a No. 8 needle, and act for 2 hours to obtain hollow chitosan pellets. Wash with water and set aside.
[0023] The transgenic strains prepared in Example 1 were cultivated to a bacterial concentration of 2.5*10 9 individual / mL. Prepare the immobilized blank pellets prepared above, the wet bacteria collected by centrifugation, and the mass volume ratio of sterilized physiological saline 1g: 1g: 100ml to prepare a bacterial suspension, absorb for 2h, take out the chitosan balls and wash them with sterilized physiological saline After that, the corresponding immobilized microspheres can be obtained.
Embodiment 3
[0024] Example 3 Phosphorus removal effect verification
[0025] Weigh 2g of immobilized blank pellets and immobilized embedded bacteria pellets respectively, and then put them into 500ml Erlenmeyer flasks containing 200ml of wastewater with a phosphorus concentration of 20mg / L, and treat them in a shaker at 28°C and 150r / min , every certain time 0, 12, 24, 36, 48, 72 (h) take 5 microliters of wastewater, centrifuge in a centrifuge, take the supernatant, and measure the total Phosphorus content, calculate phosphorus removal rate. The result is as follows:
[0026]
[0027] As can be seen from the above table, compared with the original bacterial strain, the bacterial strain provided by the present invention can achieve the effect of highly efficient phosphorus degradation in a shorter period of time, and the final phosphorus removal rate is higher, up to 99.86% than the original 96.31% before the introduction of the gene has a relatively large increase. It is more suitab...
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