Sargentodoxae caulis proantho cyanidins and preparation method thereof

A technology of proanthocyanidins and anthocyanidins, applied in the direction of organic chemistry, etc., can solve the problems such as the preparation method of anthocyanidins from the vines that have never been seen before, and achieve the effect of great practical significance and promotion value.

Inactive Publication Date: 2017-06-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are isolated and purified proanthocyanidins B from S. 2 and a report of a proanthocyanidin 5-mer, but there is no report on the preparation method of the proanthocyanidins of the genus S.

Method used

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  • Sargentodoxae caulis proantho cyanidins and preparation method thereof
  • Sargentodoxae caulis proantho cyanidins and preparation method thereof
  • Sargentodoxae caulis proantho cyanidins and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation, ultraviolet and infrared spectroscopic analysis and content determination of a kind of proanthocyanidin

[0032] Weigh 50g of dry Rhizoma sagittata coarse powder, add 500mL of 70% acetone, in an ultrasonic generator, ultrasonically extract at 40KHz for 30min, filter with Buchner funnel, extract 3 times in total, combine the filtrates for 3 times, and put the filtrate in rotary evaporation Concentrate under reduced pressure at 38°C in a container to obtain the concentrated solution of Sargentina radix, put the concentrated solution in a separatory funnel, extract 3 times with petroleum ether, collect the water layer, and obtain the degreased sageteng concentrated solution, the concentrated solution is diluted with 0.45 μm microporous membrane filtration, used for Sephadex LH-20 purification.

[0033] Take 100g of Sephadex LH-20 filler, fully swell with 50% volume fraction of methanol, wet-pack the column, and then equilibrate the column with 1200mL 50% volum...

Embodiment 2

[0055] Preparation of a kind of Rhododendron proanthocyanidin

[0056] Weigh 100g of dry S. sanctana coarse powder, add 1000mL of 65% ethanol, reflux extraction at 60°C for 3 times, extract for 1 hour each time, filter, and concentrate the filtrate in a rotary evaporator at 50°C under reduced pressure to obtain S. Concentrated solution, put the concentrated solution in a separatory funnel, extract 3 times with petroleum ether, collect the water layer, and obtain the degreased Rhizoma sagittarius concentrated solution, filter the concentrated solution with a 0.45 μm microporous membrane filter, and use it for Sephadex LH- 20 purification.

[0057] Take 100g of Sephadex LH-20 filler, fully swell with 50% volume fraction of methanol, wet-pack the column, and then equilibrate the column with 1200mL 50% volume fraction of methanol. , eluted with 50% volume fraction of methanol to fully remove polysaccharides, peptides, saponins, terpenes, flavonoids and small molecule phenols unti...

Embodiment 3

[0061] Preparation of a kind of Rhododendron proanthocyanidin

[0062] Weigh 100g of dry Rhizoma sagittata coarse powder, add 1000mL of 70% acetone, stand and extract for 3 times, extract for 24 hours each time, filter, and concentrate the filtrate under reduced pressure in a rotary evaporator at 40°C to obtain the concentrate of Rhizoma sagittata , put the concentrated solution in a separatory funnel, extract 3 times with petroleum ether, collect the water layer, and obtain the degreased Rhizoma sagittata concentrated solution, which is filtered through a 0.45 μm microporous membrane and used for Sephadex LH-20 purification .

[0063] Take 100g of Sephadex LH-20 filler, fully swell with 50% volume fraction of methanol, wet-pack the column, and then equilibrate the column with 1200mL 50% volume fraction of methanol. , eluted with 50% volume fraction of methanol to fully remove polysaccharides, peptides, saponins, terpenes, flavonoids and small molecule phenols until the eluen...

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Abstract

The invention discloses sargentodoxae caulis proantho cyanidins and a preparation method thereof. The sargentodoxae caulis proantho cyanidins is obtained by performing crushing, extraction and Sephadex LH-20 purification on stems and vines of sargentodoxacuneata, and a plant of lardizabalaceae; the sargentodoxae caulis proantho cyanidins prepared by the method disclosed by the invention has the advantages that the raw materials are easy to obtain, the preparation method is simple and can achieve large-scale preparation, and the prepared proantho cyanidins is high in purity; the invention fills up a blank in the sargentodoxae caulis proantho cyanidins and the preparation method thereof, and has a great practical significance and a great promotion value.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and relates to a sagittaria proanthocyanidin and a preparation method thereof. [0002] technical background [0003] Sargentodoxae Caulis, also known as red vine and blood vine, is the stem vine of Sargentodoxacuneata, which is harvested in autumn and winter, removed side branches, sliced ​​and dried. It is mainly produced in Sichuan, Henan, Jiangxi, Hubei, Hunan, Jiangsu, Zhejiang and other places in China. Sacred Teng is neutral in nature and bitter in taste, and returns to the Liver, Stomach, Pericardium, and Large Intestine Meridian. It has the functions of clearing away heat and detoxification, promoting blood circulation and promoting menstrual flow, expelling wind and killing insects, reducing swelling and dispelling stagnation, etc. It is used for rheumatism, muscle pain, amenorrhea and dysmenorrhea. Intestinal carbuncle and other diseases are commonly used traditional Chinese m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 李彦李鑫梁宗锁
Owner NORTHWEST A & F UNIV
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