Detection method for folic acid concentration for maintaining human lymphocyte cell genome stability in vitro

A technology for genome stability and lymphocytes, applied in the field of in vitro maintenance of human lymphocyte genome stability folic acid concentration detection, can solve problems such as chromosome breakage, centromere heterochromatin condensation level, gene expression changes, etc., to achieve maintenance The effect of stability
CN106834409AInactive Publication Date: 2017-06-13YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2017-06-13
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to the technical field of molecular biology and in particular relates to a detection method for folic acid concentration for maintaining human lymphocyte cell genome stability in vitro. The detection method comprises the following steps: designing a culture medium; separating lymphocyte cells; culturing the lymphocyte cells; collecting the cells and preparing a sheet; analyzing the sheet; carrying out statistic analysis. The human peripheral blood lymphocyte cells are cultured in vitro and different folic acid concentrations are set; the optimal concentration for maintaining the human lymphocyte cell genome stability in vitro is detected through CBMN (Cytokinesis Block Micronucleus) Cyt and the measured optimal concentration for maintaining the human lymphocyte cell genome stability in vitro is 120nmol / L; a result is closer to a living body and important evidences are provided for nutrition intervention of individuals and effective maintenance of the individual genome stability.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology, in particular to a method for detecting the concentration of folic acid for maintaining the genome stability of human lymphocytes in vitro. Background technique

[0002] The genome carries all the biological information necessary for the formation and maintenance of human life, enabling humans to not only carry out various life activities normally in the long-term evolution process, but also to stably inherit various traits adapted to the environment to future generations. The relative stability of the human genome is taken as a premise. With the in-depth study of epigenetic phenomena and the continuous expansion of genome information knowledge, people have discovered that in addition to gene mutations, chromosomal aberrations, gene dosage changes caused by aneuploidy, and chromosome breakage-fusion-bridge (breakage-fusion-bridge) , BFB) cycles lead to gene amplification and other geno...

Claims

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