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Method for screening anaerobe traditional Chinese medicine inhibitor on basis of ZnO nanoprobe

A technology of nano-probes and anaerobic bacteria, which is applied in the direction of measuring devices, instruments, and nuclear magnetic resonance analysis, and can solve problems such as the decrease of nuclear magnetic resonance relaxation time

Inactive Publication Date: 2017-06-13
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned earlier, a small amount of ZnO nanomaterials will cause a significant decrease in the NMR relaxation time of water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on acne bacilli (anaerobic bacteria).

[0020] 1) Nano-ZnO immunoprobe preparation:

[0021] ZnO nanoparticles can be purchased from the market, or can be synthesized by physical or chemical methods with reference to literature.

[0022] Nano-ZnO coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-ZnO and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three times to ens...

example 2

[0027] To screen traditional Chinese medicine formulas that have inhibitory or therapeutic effects on Clostridium difficile (anaerobic bacteria).

[0028] 1) Nano-ZnO immunoprobe preparation:

[0029] ZnO nanoparticles can be purchased from the market, or can be synthesized by physical and chemical methods with reference to literature.

[0030] Nano-ZnO coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-ZnO and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85˚C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of methanol. The above process is repeated three ...

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Abstract

The invention relates to a method for screening an anaerobe traditional Chinese medicine inhibitor on basis of a ZnO nanoprobe and belongs to the technical field of traditional Chinese medicine development and application. The method depends on establishment of a method based on the prepared ZnO nanoprobe and used for screening a traditional Chinese medicine formula for inhibiting anaerobes. Whether a sample contains target bacteria is detected by using the characteristic that the antibody-coated magnetic ZnO nanoprobe can perform specific combination on the target bacteria and by using the effect of the magnetic characteristic of ZnO on nuclear magnetic resonance relaxation time. The magnetic ZnO nanoprobe has a linear relation with the nuclear magnetic resonance relaxation time within a certain range, that is, the higher the nano ZnO content, the shorter the nuclear magnetic resonance spin-lattice relaxation time and spin-spin relaxation time of the sampler is, the target bacteria can be detected quantitatively in a certain range, and the antibacterial effect of the traditional Chinese medicine formula is indirectly evaluated. The method can be used for screening the traditional Chinese medicine formula having the inhibition effect on the anaerobes and increase the development speed of traditional Chinese medicine products.

Description

technical field [0001] The invention relates to a screening method of traditional Chinese medicine formula. Background technique [0002] Antibiotics can also cause damage to the human body while sterilizing bacteria, affecting liver, kidney function, and gastrointestinal tract reactions. Abuse of antibiotics may lead to the occurrence of drug resistance in some bacteria, and the treatment of infections will become very difficult. At present, there is almost no antimicrobial drug without drug resistance. It is of positive significance to develop traditional Chinese medicines and formulas with antibacterial effects. [0003] Many traditional Chinese medicines and prescription medicines have antibacterial and anti-inflammatory effects, and the active ingredients and molecular structures of some Chinese herbal medicines have also been fully or partially studied. For example, berberine (berberine), the main component of Coptis chinensis and Phellodendron phellodendron for dys...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/088
Inventor 张锦胜巫小丹万益琴彭红郑洪立刘玉环王允圃
Owner NANCHANG UNIV
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