Fast 3D Microscopic Imaging System

Abstract

The present invention proposes a fast three-dimensional microscopic imaging system, including: a microscope, which magnifies the microscopic sample and images it to the outlet of the image plane; It is used for narrow-band filtering of the information from the sample; the beam splitting grating is used to split the output image of the microscope after passing through the first-stage 4f system into beams in two-dimensional space; the multi-focal lens array is used to add different phases to each beam Modulation to obtain images of different depths on the rear focal plane of the subsequent lens; microlens arrays are used to modulate light rays of different angles passing through the beam splitting grating and multi-focal lens arrays to different spatial positions behind the microlenses; image sensors are used to Record the sample image modulated by the previous stage. The invention can realize multi-depth simultaneous imaging at the single imaging speed of an ordinary wide-field microscope, has fast imaging speed and high image resolution, can be used for living samples, and has a simple system structure and easy adjustment of imaging depth intervals.

Description

technical field [0001] The invention relates to the technical field of computational photography, in particular to a fast three-dimensional microscopic imaging system. Background technique [0002] Life science and material science research put forward higher requirements for 3D microscopic imaging. At present, most microscopic three-dimensional imaging uses confocal microscopes or two-photon and multiphoton microscopes to scan and collect fluorescent samples point by point to form images at different depths. However, these methods have two disadvantages. First, the required excitation light is strong, which is likely to cause fluorescence bleaching, and strong light irradiation may damage sample cells or tissue structures; second, imaging a specific depth of a fluorescent sample requires point-by-point scanning, which is inefficient in time. Therefore, the above methods are not suitable for photosensitive fluorescent samples and biological samples, and the imaging speed i...

AI Technical Summary

Problems solved by technology

However, the use of wide-field microscopy to obtain fluorescence images has the following problems: the excitation light not only excites the fluorescence signal at the focal plane of the objective lens, but also excites the fluorescence signal at the non-focal plane. Fluorescence intensity and non-focal plane fluorescence intensity and noise superposition, and the image resolution obtained at each depth is lower than that of the previous method

However, this method sacrifices the spatial resolution, resulting in a lower three-dimensional resolution of the computationally reconstructed sample, and for thicker samples, the reconstruction quality is poor.

The multi-plane simultaneous imaging method based on multi-focal grating adds beam splitting and phase modulation to the Fourier plane at the same time, but the system structure is complicated to solve the dispersion problem

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