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Production method and product of novel multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film

A technology of polyethylene glycol diacrylate and multi-walled carbon nanotubes, applied in the field of biomedicine, can solve the problems of poor mechanical properties of natural materials, lack of receptors for cell recognition, etc., and achieve strong operability and simple process Effect

Active Publication Date: 2017-06-16
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Natural and synthetic materials have been used to construct three-dimensional matrices for tissue engineering, however, natural materials often have poor mechanical properties, while synthetic materials often lack receptors for cell recognition

Method used

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  • Production method and product of novel multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film
  • Production method and product of novel multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film
  • Production method and product of novel multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preparation of multi-walled carbon nanotube-dopamine-polyethylene glycol diacrylate hydrogel film, the specific steps are as follows:

[0028] (1) Add N,N-methylenebisacryloylamine (MBA) into the ethanol solution to obtain the MBA solution, wherein the pH value of the ethanol solution is 6, and the volume ratio of water to ethanol in the ethanol solution is 4 : 3, MBA concentration is 70.1mg / ml in the MBA solution that makes;

[0029] (2) Add dopamine to the MBA solution prepared in step (1) under nitrogen protection according to the molar ratio of dopamine to MBA in the MBA solution of 2:3, stir at 45°C in the dark for 3 days, and freeze-dry to prepare dopamine-MBA Cross-linking agent, the prepared dopamine-MBA cross-linking agent is stored at 20°C; 1 H NMR nuclear magnetic resonance spectroscopy identifies the chemical structure of the dopamine-MBA crosslinker, the results are as follows figure 1 shown by figure 1 It can be seen that 5.7 and 6.2 ppm represent vinyl...

Embodiment 2

[0034] Preparation of multi-walled carbon nanotube-dopamine-polyethylene glycol diacrylate hydrogel film, the specific steps are as follows:

[0035] (1) Add N,N-methylenebisacryloylamine (MBA) into the ethanol solution to obtain the MBA solution, wherein the pH value of the ethanol solution is 6, and the volume ratio of water to ethanol in the ethanol solution is 4 : 3, MBA concentration is 70.1mg / ml in the MBA solution that makes;

[0036] (2) Add dopamine to the MBA solution prepared in step (1) under the protection of nitrogen according to the molar ratio of dopamine to MBA in the MBA solution of 2:3, stir at 50°C in the dark for 2 days, and freeze-dry to prepare dopamine-MBA Cross-linking agent, store the prepared dopamine-MBA cross-linking agent at 20°C;

[0037] (3) adding multi-walled carbon nanotubes into a poloxamer solution (F127 solution) with a mass volume percentage of 10% to prepare a multi-walled carbon nanotube solution, the multi-walled carbon nanotubes in t...

Embodiment 3

[0041] Preparation of multi-walled carbon nanotube-dopamine-polyethylene glycol diacrylate hydrogel film, the specific steps are as follows:

[0042] (1) Add N,N-methylenebisacryloylamine (MBA) into the ethanol solution to obtain the MBA solution, wherein the pH value of the ethanol solution is 6, and the volume ratio of water to ethanol in the ethanol solution is 4 : 3, MBA concentration is 70.1mg / ml in the MBA solution that makes;

[0043] (2) Add dopamine to the MBA solution prepared in step (1) under the protection of nitrogen according to the molar ratio of dopamine to MBA in the MBA solution of 2:3, stir at 55°C in the dark for 1 day, and freeze-dry to prepare dopamine-MBA Cross-linking agent, store the prepared dopamine-MBA cross-linking agent at 20°C;

[0044](3) adding multi-walled carbon nanotubes into a poloxamer solution (F127 solution) with a mass volume percentage of 10% to make a multi-walled carbon nanotube solution, the multi-walled carbon nanotubes in the mu...

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Abstract

The invention relates to a production method and a product of a novel multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film. The method concretely comprises the following steps: 1, adding dopamine into a cross-linking agent solution under an anoxic condition, stirring the dopamine and the cross-linking agent solution at 45-55 DEG C in a shady place for 1-3 d, and freeze-drying the obtained solution to obtain a dopamine cross-linking agent; 2, adding a multiwalled carbon nanotube solution to the dopamine cross-linking agent, uniformly mixing the multiwalled carbon nanotube solution and the dopamine cross-linking agent, and adding polyethyleneglycol diacrylate and a photoinitiator to the obtained mixed solution to prepare a precursor solution; and 3, preparing the hydrogel from the precursor solution. The multiwalled carbon nanotube-dopamine-polyethyleneglycol diacrylate hydrogel film produced in the invention self-folds during transition from a dry environment to a water-containing environment, is independent from the concentration of the multiwalled carbon nanotube-dopamine, has the characteristics of self coiling, conductivity, cell adhesion ability and biocompatibility, affects cell differentiation, has a bone- and nerve tissue-like form after self-coiling, and has application potential in the biomedical science field.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and product of a novel multi-walled carbon nanotube-dopamine-polyethylene glycol diacrylate hydrogel film. Background technique [0002] Currently the most effective and popular approach for tissue regeneration is to build a three-dimensional (3D) matrix to provide a stable tissue-mimicking environment for cell proliferation and differentiation. The ideal matrix or cell living environment should have suitable special structure, mechanical properties, biocompatibility and permeability. For an artificial 3D matrix, it is a challenge to meet all the above requirements. Natural and synthetic materials have been used to construct 3D matrices for tissue engineering, however, natural materials often have poor mechanical properties, while synthetic materials often lack receptors for cell recognition. Therefore, there is an urgent need for an artific...

Claims

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Application Information

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IPC IPC(8): C08L71/02C08K7/24C08K5/20C08J5/18C08J3/075
CPCC08J3/075C08J5/18C08J2371/02C08J2471/02C08K2201/011C08L71/02C08L2203/02C08L2203/16C08L2205/025C08K7/24C08K5/20
Inventor 李健温钰祥邢孟秋吴玉章
Owner ARMY MEDICAL UNIV
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