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Strawberry tissue and seedling culture method

A strawberry and seed technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as plant quality decline and low reproduction efficiency, and achieve the effects of easy operation, good market prospects and short cycle times.

Inactive Publication Date: 2017-06-23
李茂兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, strawberries are mainly propagated by seeds and divisions, which have low reproduction efficiency and tend to cause a decline in plant quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for group seedling cultivation of strawberries, soaking the seeds of strawberries in a potassium permanganate solution with a mass concentration of 1%, and then washing them with sterile water for 3 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the primary medium is MS + sucrose 8 g / L for primary culture, 20°C, humidity 60%, light 2000lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants , and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + indole acetic acid 0.02 mg / L + sucrose 8 g / L, at 25°C, humidity 70%, light 6000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+indole acetic acid 0.5 mg / L+sucrose 20 g / L, at 28°C, humidity 80%, and light 1000...

Embodiment 2

[0022] A method for group seedling cultivation of strawberries, soaking the seeds of strawberries in a potassium permanganate solution with a mass concentration of 3%, and then washing them with sterile water for 5 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the primary medium is MS + sucrose 8 g / L for primary culture, 22 ° C, humidity 70%, light 5000 lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings were used as explants and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + indole acetic acid 0.02 mg / L + sucrose 8 g / L, at 28°C, humidity 80%, Illuminate 8000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+indole acetic acid 0.5 mg / L + 20 g / L sucrose, 30°C, 90% humidity, and 1500...

Embodiment 3

[0024] A method for group seedling cultivation of strawberries, soaking the seeds of strawberries in a potassium permanganate solution with a mass concentration of 2%, and then washing them with sterile water for 4 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the first-generation medium is MS + sucrose 8 g / L, and the first-generation culture is carried out at 25°C, humidity 70%, and light 3000 lx to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants , and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + indole acetic acid 0.02 mg / L + sucrose 8 g / L, 28°C, humidity 75%, light 7000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+indole acetic acid 0.5 mg / L+sucrose 20 g...

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PUM

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Abstract

The invention discloses a strawberry tissue and seedling culture method. The strawberry tissue and seedling culture method is characterized in that strawberry seeds are soaked in a potassium permanganate solution with the mass concentration of 1-3%, and then sterile water is utilized to flush the soaked strawberry seeds to obtain sterile seeds; the sterile seeds are inoculated into a primary culture medium for primary culture to obtain sterile seedlings; cotyledons and hypocotyls of the sterile seedlings are taken as explants and inoculated into a subculture medium for subculture to obtain rootless seedlings; the rootless seedlings are transplanted into a rooting culture medium for rooting culture to obtain strawberry seedlings. The strawberry tissue and seedling culture method is high in rooting efficiency and survival rate and can maintain the favorable quality of plants.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and seedling cultivation, and in particular relates to a method for group cultivation of strawberry seedlings. Background technique [0002] Strawberries are rich in nutritional value and known as the "Queen of Fruits". They are rich in vitamin C, vitamin A, vitamin E, vitamin PP, vitamin B1, vitamin B2, carotene, tannic acid, aspartic acid, copper, strawberry amine , pectin, cellulose, folic acid, iron, calcium, ellagic acid and anthocyanins and other nutrients, especially the vitamin C contained in it, its content is 7-10 times higher than that of apples and grapes. The content of malic acid, citric acid, vitamin B1, vitamin B2, carotene, calcium, phosphorus, and iron is 3 to 4 times higher than that of apples, pears, and grapes. [0003] At present, strawberries are mainly propagated by seeds and divisions, which have low reproduction efficiency and are likely to cause a decline i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 李茂兰蒋军
Owner 李茂兰
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