Comprehensive preparation and purification method for polysaccharides with different properties in hericium erinaceus

A purification method and Hericium erinaceus technology are applied in the field of natural product preparation, which can solve the problems of low extraction yield and waste of raw materials, and achieve the effects of high yield, high viscosity, and good sugar content and uniformity.

Inactive Publication Date: 2017-06-23
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, hot water extraction is the most commonly used extraction method, which has the characteristics of low cost, easy operation, and is c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]The dry fruiting bodies of Hericium erinaceus were used as raw materials, which were pulverized by a pulverizer to obtain powder; 15 times of ethanol (95% ethanol mass fraction) was added to Hericium erinaceus powder, soaked for 24 h, filtered through gauze, the precipitate was collected, and the ethanol was evaporated to dryness. The obtained dry powder of Hericium erinaceus is set aside. Hericium erinaceus powder was mixed with distilled water at a weight / volume (g / mL) ratio of 1:15, extracted in a boiling water bath for 2 h, the extract was filtered with gauze, centrifuged at 4800 r / min for 15 min, and the filter residue was collected; Extraction, the extract was filtered through gauze again, centrifuged at 4800 r / min for 15 min to obtain the filtrate, the filtrate obtained from the two extractions was combined, and the filtrate and filter residue were collected respectively. The filtrate was used for the preparation of water-soluble refined polysaccharides from Heric...

Embodiment 2

[0037] The dry fruiting bodies of Hericium erinaceus were used as raw materials, which were pulverized by a pulverizer to obtain powder; 15 times of ethanol (95% ethanol mass fraction) was added to Hericium erinaceus powder, soaked for 24 h, filtered through gauze, the precipitate was collected, and the ethanol was evaporated to dryness. The obtained dry powder of Hericium erinaceus is set aside. Hericium erinaceus powder was mixed with distilled water at a weight / volume (g / mL) ratio of 1:15, extracted in a boiling water bath for 2 h, the extract was filtered with gauze, centrifuged at 4800 r / min for 15 min, and the filter residue was collected; Extraction, the extract was filtered through gauze again, centrifuged at 4800 r / min for 15 min to obtain the filtrate, the filtrate obtained from the two extractions was combined, and the filtrate and filter residue were collected respectively. The filtrate was used for the preparation of water-soluble refined polysaccharides from Heri...

Embodiment 3

[0039]The dry fruiting bodies of Hericium erinaceus were used as raw materials, which were pulverized by a pulverizer to obtain powder; 15 times of ethanol (95% ethanol mass fraction) was added to Hericium erinaceus powder, soaked for 24 h, filtered through gauze, the precipitate was collected, and the ethanol was evaporated to dryness. The obtained dry powder of Hericium erinaceus is set aside. Hericium erinaceus powder was mixed with distilled water at a weight / volume (g / mL) ratio of 1:30, extracted in a boiling water bath for 2 h, the extract was filtered with gauze, centrifuged at 4800 r / min for 15 min, and the filter residue was collected; Extraction, the extract was filtered through gauze again, centrifuged at 4800 r / min for 15 min to obtain the filtrate, the filtrate obtained from the two extractions was combined, and the filtrate and filter residue were collected respectively. The filtrate was used for the preparation of water-soluble refined polysaccharides from Heric...

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Abstract

The invention provides a comprehensive preparation and purification method for polysaccharides with different properties in hericium erinaceus. The comprehensive preparation and purification method comprises the following steps: (1) crushing dried hericium erinaceus sporophores, degreasing and drying; (2) extracting with hot water and carrying out ethanol precipitation; (3) washing sediment with absolute ethyl alcohol, acetone and absolute ethyl ether; removing protein by a Sevag method; dialyzing and removing small molecular substances to obtain hericium erinaceus water extracted water-soluble polysaccharides; (4) re-dissolving; carrying out ethanol fractional precipitation to obtain hericium erinaceus water-soluble purified polysaccharide components with different properties; (5) drying filter residue generated after extracting with the hot water; extracting with NaOH/NaBH4, and carrying out the ethanol precipitation; washing with the absolute ethyl alcohol, the acetone and the absolute ethyl ether; removing protein by the Sevag method; dialyzing and removing small molecular substances to obtain hericium erinaceus alkali extracted water-soluble polysaccharides. According to the comprehensive preparation and purification method, the water extracted water-soluble polysaccharides and high-stickiness alkali extracted water-soluble polysaccharides of the hericium erinaceus are prepared from the hericium erinaceus sporophores, and the utilization rate is high; the water extracted water-soluble polysaccharides of the hericium erinaceus are subjected to fractionation purification through the ethanol to obtain the hericium erinaceus water-soluble purified polysaccharide components with different properties; a process is simple, and the cost is low.

Description

technical field [0001] The invention belongs to the field of natural product preparation. Background technique [0002] Hericium erinaceus ( Hericium erinaceus ), also known as Hericium erinaceus, hedgehog fungus, cauliflower fungus or mountain burdock fungus, etc., including Hericium erinaceus, small thorny Hericium erinaceus, pseudo-Hericium erinaceus and coral-shaped Hericium erinaceus. Rotten wood on leaves and wounds on standing trees are widely distributed in East Asia. Hericium erinaceus has been recorded in "Compendium of Materia Medica": Hericium erinaceus is flat in nature, sweet in taste, and has the function of "benefiting the five internal organs and helping digestion". "Bear's paw, sea cucumber, and shark's fin" are one of the four famous dishes. It is known as "vegetarian meat". As a medicinal and edible fungus, Hericium erinaceus has rich nutritional value and medicinal value, and can be used to assist in the treatment of gastric ulcer, indigestion, gastri...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/00C08B37/0003
Inventor 谢明勇王小银殷军艺聂少平
Owner NANCHANG UNIV
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