Method for detecting five tauro-conjugated bile acids in serum by high performance liquid chromatography-tandem mass spectrometry

A high-performance liquid chromatography, taurine-binding type technology is applied in the field of high-performance liquid chromatography tandem mass spectrometry detection of five kinds of taurine-binding-type bile acids, which can solve the problems of insufficient sensitivity and specificity, and complicated detection operations of taurine-binding-type bile acids. and other problems, to achieve the effect of good specificity, removal of serum matrix interference, and high throughput

Inactive Publication Date: 2017-06-23
HANGZHOU BAICHEN MEDICAL LAB CO LTD
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Problems solved by technology

[0005] In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a method for detecting five kinds of taurine-conjugated bile acids in serum by high performance liquid chromatography tandem mass spectrometry. The detection operation of taurine-conjugated bile acids is complex, and the sensitivity and specificity Insufficient problem

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  • Method for detecting five tauro-conjugated bile acids in serum by high performance liquid chromatography-tandem mass spectrometry
  • Method for detecting five tauro-conjugated bile acids in serum by high performance liquid chromatography-tandem mass spectrometry
  • Method for detecting five tauro-conjugated bile acids in serum by high performance liquid chromatography-tandem mass spectrometry

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[0028] Below, in conjunction with accompanying drawing and specific embodiment, the present invention is described further:

[0029] Five taurine-conjugated bile acids (taurocholic acid, taurolithocholic acid, taurodeoxycholic acid, tauroursodeoxycholic acid, taurochenodeoxycholic acid) standards and isotope-labeled internal standards (deuterium Cholic acid, deuterated ursodeoxycholic acid) were purchased from Sigma Aldrich. The standard and internal standard are firstly dissolved in acetonitrile, and then stored at -80°C. When the actual sample detection is required, the internal standard deuterated cholic acid is diluted with acetonitrile to a concentration of 76nmol / L, and the internal standard deuterated ursodeoxycholic acid The dilution concentration is 126nmol / L, as the working solution.

[0030] (1) Standard product preparation: Take 0.1mL of 9 acetonitrile solutions of taurine-conjugated bile acids with different concentrations into 1.5mL EP tubes, and then add 0.3mL ...

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Abstract

The invention discloses a method for detecting five kinds of taurine-bound bile acids in serum by using high performance liquid chromatography tandem mass spectrometry, comprising the following steps: preparation of standard products, separation by high performance liquid chromatography, detection by tandem mass spectrometry, taurine in serum Detection of conjugated bile acids, qualitative and precise quantitative detection of five kinds of taurine-conjugated serum including tauroursodeoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, taurodeoxycholic acid and taurolithocholic acid Type bile acid, short detection time, high efficiency, high detection sensitivity, good specificity, and low cost.

Description

technical field [0001] The invention relates to the field of bile acid detection, in particular to a method for detecting five kinds of taurine-conjugated bile acids by high-performance liquid chromatography tandem mass spectrometry. Background technique [0002] Taurine-conjugated bile acids refer to the combination of bile acids with taurine through amide bonds (referred to as peptide bonds) to form taurine-conjugated bile acids, including taurocholic acid (TCA), taurodeoxycholic acid (taurochenodeoxycholic, TCDCA), taurolithocholic acid (taurolithocholic acid, TLCA), taurodeoxycholic acid (taurodesoxycholic acid, TDCA), tauroursodeoxycholic acid (tauroursodeoxycholic acid, TUDCA). [0003] Taurine-bound bile acids are easily soluble in water, because their molecules contain not only hydrophilic hydroxyl and carboxyl groups, but also hydrophobic methyl groups, and these two groups with different properties are completely arranged in cyclopentane. The two sides of the hydr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06G01N2030/884
Inventor 吴超超
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD
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