A method for extracting, separating and purifying blueberry anthocyanins

A technology for the separation and purification of blueberry anthocyanins, which is applied in the field of extracting, separating and purifying blueberry anthocyanins using normal temperature and high pressure homogenization technology, can solve problems such as high equipment cost, insufficient product quality and market competitiveness, and poor safety, and achieve Rapid results of separation and purification methods

Active Publication Date: 2020-06-12
EN SNOW FOODS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, although the above-mentioned extraction, separation and purification methods related to blueberry fruit anthocyanins can prepare blueberry anthocyanin extracts with certain purity and yield, due to the limitation of technical means and the use of a large amount of toxic organic solvents or chemical preparations in the process , so that the product obviously has defects such as poor safety or low purity and yield, high equipment cost, and insufficient product quality and market competitiveness.

Method used

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  • A method for extracting, separating and purifying blueberry anthocyanins
  • A method for extracting, separating and purifying blueberry anthocyanins
  • A method for extracting, separating and purifying blueberry anthocyanins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Blueberry anthocyanin extraction process research

[0064] Taking blueberry anthocyanins as the measurement index, taking extraction pressure, solid-liquid ratio, and extraction times as the investigation index factors, the extraction efficiency of blueberry anthocyanins was systematically investigated. First, the effects of pressure, solid-liquid ratio, and extraction times on the anthocyanin Extract content single factor experiment, then conduct L9(3 3 ) content orthogonal experimental design, optimize the extraction process at room temperature and high pressure. Find out the primary and secondary order of blueberry anthocyanin extraction rate, and propose the optimal extraction scheme.

[0065] 3.1 Orthogonal experiment

[0066] The fixed blueberry juice sample mass is 10.0 g, the solution pH is 1.0, and the extractant is 80% ethanol solution. Select pressure, material-liquid ratio, and number of experiments as the influencing factors, and design an orth...

Embodiment 2

[0114] Example 2 Research on the separation and purification method of blueberry anthocyanins

[0115] The crude extract of blueberry anthocyanins was purified by using silica gel, polyamide resin, and HPD600 macroporous adsorption resin as fillers for column chromatography. Taking the anthocyanin content and anthocyanin yield in each gradient eluent obtained from different resins as the measurement index, the column chromatography filler for purifying anthocyanin was screened. In this experiment, ethanol solution was used as the eluent.

[0116] 4.1 Resin pretreatment

[0117] Silica gel: Chromatographic silica gel should be neutral and colorless particles, but because the production process is often acidic, you should also check whether it contains iron ions. a. Iron ion inspection: Take an appropriate amount of silica gel, suspend it in hydrochloric acid, stir, if it contains iron ions, it will combine with hydrochloric acid to form a complex and appear yellow. At this tim...

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Abstract

The invention relates to an extraction, separation and purification method for blueberry anthocyanins. The method comprises extraction steps as follows: S1, blueberries are washed and juiced for later use; S2, blueberry juice obtained in S1 is mixed with an extracting agent in the solid-liquid ratio being (1:10)-(1:25), the mixture is subjected to homogenization extraction 1-4 times at the normal temperature and under the pressure of 100-160 Mpa and filtered, filtrates are mixed, and a blueberry anthocyanin crude extract is obtained. By means of the method, more functional ingredients in blueberry raw materials can be dissolved in a solvent, the functional ingredients of the natural product can be released from biological cells under the action of high pressure, and the method for obtaining the blueberry anthocyanins is safer and rapider.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and specifically relates to a green chemical process technology for extracting, separating and purifying anthocyanins from blueberries, and more specifically relates to a method for extracting, separating and purifying blueberry anthocyanins by using normal temperature and high pressure homogenization technology. Background technique [0002] Blueberry, also known as lingonberry and blueberry, belongs to the perennial deciduous or evergreen shrub of Rhododendronaceae Vaccinium. It mainly has four types: low bush, high bush, rabbit eye and half-high bush. The fruit is oblate and dark blue when ripe. Blueberry fruit is rich in anthocyanins, and also contains carbohydrates, organic acids, cellulose, Vc, flavonoids, potassium, calcium, phosphorus and other minerals. Because blueberry fruit is rich in anthocyanins, low in sugar, low in fat, and has strong antioxidant capacity, it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/065C07H1/08C09B61/00C09B67/54
CPCC07H1/08C07H17/065C09B61/00C09B67/0096
Inventor 丘敬华陈思奇何伟勤梁勇陈忻陈晓刚
Owner EN SNOW FOODS CO LTD
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