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Method for preparing lysozyme by means of fermentation process

A fermentation process and lysozyme technology, applied in the field of bioengineering, can solve problems such as limited application, limited lysozyme activity, and less research on lysozyme activity

Active Publication Date: 2017-06-30
ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Prior art At present, the research on the medium used in the fermentation process to prepare lysozyme is mainly limited to the selection of nitrogen source, carbon source and other components. There are few studies on how the type and concentration of surfactant affect the activity of lysozyme, and the existing culture The activity of lysozyme produced by basal fermentation is limited, resulting in limited application of this type of lysozyme method in actual industrial production

Method used

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  • Method for preparing lysozyme by means of fermentation process

Examples

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Embodiment 1

[0023] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L, coconut oil fatty acid diethylamide 1g / L, Tween 80 0.5g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Bacillus subtilis K-77 by 4% (v / v) inoculum amount, carry out shaking flask fermentation, fermentation condition is 30 ℃, 200r / min, After 24 hours of fermentation and cultivation, lysozyme L1 was obtained.

Embodiment 2

[0025] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L, coconut oil fatty acid diethylamide 0.75g / L, Tween 80 0.75g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Bacillus subtilis K-77 by 4% (v / v) inoculum amount, carry out shaking flask fermentation, fermentation condition is 30 ℃, 200r / min, After 24 hours of fermentation and cultivation, lysozyme L2 was obtained.

Embodiment 3

[0027] Prepare liquid culture medium, wherein glucose 10g / L, peptone 5g / L, coconut oil fatty acid diethylamide 0.5g / L, Tween 80 1g / L. Get 25mL liquid culture medium and place in Erlenmeyer flask, insert the thalline seed of Bacillus subtilis K-77 by 4% (v / v) inoculum amount, carry out shaking flask fermentation, fermentation condition is 30 ℃, 200r / min, After 24 hours of fermentation and cultivation, lysozyme L3 was obtained.

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Abstract

The invention relates to a method for preparing lysozyme by means of a fermentation process. The method includes the following steps that a liquid culture medium is placed in a triangular flask, bacteria are inoculated with the inoculation amount of 1-10% v / v, shaking flask fermentation is conducted at 20-40 DEG C and 150-300 r / min for 24 hours, then fermentation liquid is centrifuged to remove the bacteria, supernatant is purified by an ammonium sulfate salting-out method, and the lysozyme is obtained, wherein the liquid culture medium includes glucose, peptone and surfactant, and the surfactant is a mixture of coconut bis(2-hydroxyethyl) amine and tween type surfactant with the concentration ratio of 1:2 to 2:1. According to the fermentation process method, by optimizing the type and concentration of the surfactant in the culture medium, the lysozyme high in activity is obtained.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing lysozyme through a fermentation process. Background technique [0002] Lysozyme (Lysozyme), also known as cell wall hydrolase, can specifically act on the peptidoglycan molecule of bacterial cell wall, and can break the β-1,4 glycosidic bond between N-acetylmuramic acid and acetylglucosamine, Make the bacterial cell wall become loose to achieve the effect of lysing the bacteria. Lysozyme is widely distributed in higher animal and plant tissues and their secretions, protozoa, insects, plants and various microorganisms. [0003] As one of the non-specific immune factors in the normal body fluids and tissues of the human body, lysozyme has a variety of pharmacological effects, participates in various immune reactions of the body, has antibacterial and anti-viral effects, and plays a role in the normal defense function and non-specific immunity of the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/36C12R1/545C12R1/45C12R1/125C12R1/01
CPCC12N9/2462C12Y302/01017
Inventor 潘宏涛卢亚萍
Owner ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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