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Method for identifying production places of lychee honey

A technology of lychee honey and origin, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as unscientific and difficult, and achieve the effect of determining objective and accurate, accurate identification results, and ensuring integrity.

Active Publication Date: 2017-06-30
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is very difficult and unscientific to find a reliable marker to identify its botanical and geographical origin based on a class of compounds that were originally assumed subjectively.

Method used

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  • Method for identifying production places of lychee honey
  • Method for identifying production places of lychee honey
  • Method for identifying production places of lychee honey

Examples

Experimental program
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Embodiment 1

[0122] A method for screening characteristic markers used to identify litchi honey origins. This embodiment can screen out characteristic markers used to identify Hainan litchi honey and Yunnan litchi honey. The specific method includes the following steps:

[0123] (1) Preparation of standard samples: 5 samples from Hainan as a group and 5 samples from Yunnan as a group. Weigh 1.0±0.1g of each sample and place them in a 10mL centrifuge tube with stopper, add 5mL of pure water, vortex Spin for 5 minutes to mix well, centrifuge at 10,000rpm for 10 minutes to precipitate particulate impurities, take the supernatant, and set aside;

[0124] (2) analysis: get the standard sample that step (1) prepares, carry out HPLC-quadrupole / electrostatic field orbital hydrazine high-resolution mass spectrometry analysis respectively, and concrete analysis condition is:

[0125] High performance liquid chromatography:

[0126] Mobile phase: 0.1% formic acid water (A) and 0.1% formic acid aceto...

Embodiment 2

[0138] A method for screening characteristic markers used to identify litchi honey production areas. This embodiment can screen out characteristic markers used to identify Fujian litchi honey and Yunnan litchi honey. The specific method is the same as in Example 1. The specific analysis results are:

[0139] (1) The results of principal component analysis (PCA) showed that litchi honey from Fujian and Yunnan could be completely separated ( image 3 ), indicating that Yunnan and Fujian litchi honey had obvious metabolite changes.

[0140] (2) Comparing Fujian lychee honey with Yunnan lychee honey: Step 1: Analyze the components with significant difference in t-test with P-Value2 FC ) is the abscissa, (-log 10 P-value ) as the ordinate, and take the value of FC>10 and P-value in the variable importance projection map 1.65 Rank the metabolites. The result display (please refer to Figure 5 ): β-cyclic citral and abscisic acid can be used as the characteristic markers to dist...

Embodiment 3

[0143] A method for screening characteristic markers used to identify litchi honey production areas. This embodiment can screen out characteristic markers used to identify Fujian litchi honey and Yunnan litchi honey. The specific method is the same as in Example 1. The specific analysis results are:

[0144] (1) The results of principal component analysis (PCA) showed that litchi honey from Fujian and Yunnan could be completely separated ( image 3 ), indicating that Yunnan and Guangdong litchi honey had obvious metabolite changes.

[0145] (2) Comparing Guangdong litchi honey with Yunnan litchi honey: Step 1: Analyze the components with significant difference in t-test with P-Value2 FC ) is the abscissa, (-log 10 P-value ) as the ordinate, and take the value of FC>10 and P-value in the variable importance projection map 1.65 Rank the metabolites. The results are displayed (for details, please refer to Image 6 ): Metocetin can be used as a characteristic marker to distin...

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Abstract

The invention relates to a method for identifying production places of lychee honey. According to the method, the lychee honey in five main producing areas, namely Hainan, Yunnan, Fujian, Guangdong and Guangxi in China can be identified. The method comprises the following steps: using at least one of 3,4-dimethoxycinnamic acid, abscisic acid, alpha-curcumene and malic acid as a characteristic marker for identifying the lychee honey from Hainan and Yunnan; using at least one of beta-cyclocitral, the bscisic acid, hydroxycinnamic acid and the malic acid as a characteristic marker for identifying the lychee honey from Hainan and Yunnan; using at least one of luteolin, the hydroxycinnamic acid and the malic acid as a characteristic marker for identifying the lychee honey from Guangdong and Yunnan; using at least one of the abscisic acid, hydroxycinnamic acid and the malic acid as a characteristic marker for identifying the lychee honey from Guangxi and Yunnan. The characteristic markers are all obtained according to a non-targeting technology. Therefore, the characteristic markers are used for identifying the lychee honey from different production places, and further results are more accurate.

Description

technical field [0001] The invention relates to the technical field of honey production area identification, in particular to a method for identifying the production area of ​​lychee honey. Background technique [0002] my country is a big beekeeping country in the world. The total number of bee colonies has reached 9.1 million, and the annual output of honey exceeds 450,000 tons. It is a big country in honey production, consumption and export. Modern scientific research has proved that honey contains more than a hundred kinds of endogenous components, glucose and fructose are its main components, accounting for more than 70% of the total components, followed by water, sucrose, minerals, vitamins, proteins, amino acids, etc. In recent years, consumers have shown increasing interest in honey due to its antibacterial, anti-inflammatory, antimutagenic, antitumor, and antioxidative activities. Honey composition is closely related to its botanical and geographical origin, as hon...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72
Inventor 周金慧李熠问亚琴张文文陈兰珍张金振金玥赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI