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Composite organic nitrogen source kit

An organic nitrogen and kit technology, applied in microorganisms, fungi, bacteria, etc., can solve problems such as inability to reproduce production results, and achieve the effects of improving efficiency, saving time and cost, and reducing workload

Inactive Publication Date: 2017-07-04
王晓雨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the present invention aims at the technical problem that the R&D and production personnel in the prior art cannot reproduce the production results when replacing the compound organic nitrogen source, and the purpose is to provide a brand-new product, which is a compound organic nitrogen source kit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The compound organic nitrogen source kit of different sources of embodiment 1

[0024] This kit contains 6 different types of compound organic nitrogen sources, including cottonseed powder, peptone, dry corn steep powder, soybean cake powder, fish meal and yeast powder. The sources are: Handan Zhongmian Ziguang Cotton Industrialization Technology Co., Ltd., Shandong Yubao Biotechnology Co., Ltd., Shandong Yuncheng Kangyuan Biotechnology Co., Ltd., Shandong Wandefu Industrial Group Co., Ltd., Haixing Haizhiyuan Feed Co., Ltd. and Angel Yeast Co., Ltd.

[0025] Table 1 contains kits of 6 different compound organic nitrogen sources

[0026]

[0027]

[0028] Remarks: The number behind the compound organic nitrogen source represents the batch number.

[0029] In the production of erythromycin by the Stretomyces erythreus SL0223 strain, the research and development personnel want to select the most suitable compound organic nitrogen source from the above-mentioned 6 k...

Embodiment 2

[0030] Embodiment 2 Kit A

[0031] Kit A is composed of cottonseed powder NJ2208, a single compound nitrogen source, with a specification of 10ⅹ20g. The quantitative detection indicators provided are shown in Table 2.

[0032] Table 2 Cottonseed powder NJ2208 quantitative detection index of kit A

[0033]

[0034]

[0035]

[0036]After choosing cottonseed flour NJ2208 as the optimum nitrogen source according to Example 1, the Streptomyces strains were verified and re-verified using Kit A. The verification process is as follows: take out 5 packages of the test kit A, weigh the formula of the culture solution according to the technical requirements, add 20g cottonseed powder 2208, which is 1 package of the test kit A, and prepare the culture medium (the formula is: cornstarch 70g / L, potassium dihydrogen phosphate 0.4g / L, ammonium sulfate 2.5g / L, glucose 10g / L and calcium carbonate 5g / L, pH is 6.80), then inoculated and fermented at 31°C for 120h. Test the results af...

Embodiment 3

[0042] Example 3 Kit B

[0043] Kit B is 2 types of single compound organic nitrogen source produced by Handan Zhongmian Ziguang Cotton Industrialization Technology Co., Ltd., cottonseed powder NJ2208 with a specification of 5ⅹ20g, and cottonseed powder NJ2206 with a specification of 5ⅹ20g, each small package 20g, quantitative detection index See Table 4.

[0044] Table 4 Calibration detection index of cottonseed flour NJ2206 and cottonseed flour NJ2208

[0045]

[0046]

[0047] According to the technical requirements of R&D and production, each reagent was weighed according to the medium formula of Stretomyces erythreus SL0223 for the production of erythromycin, and the reagent formula was divided into 10 parts. The 10 packages of kit B were added to the above formula respectively to prepare a culture solution (The formula is: corn starch 70g / L, potassium dihydrogen phosphate 0.4g / L, ammonium sulfate 2.5g / L, glucose 10g / L and calcium carbonate 5g / L pH is 6.80), then i...

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Abstract

The invention discloses a composite organic nitrogen source kit. The composite organic nitrogen source kit comprises M composite organic nitrogen sources independently packaged, and each composite organic nitrogen source is marked with the contents of N quantitative indexes, wherein 1000>=M>=1, 2000>=N>=5, preferably, preferably, 20>=M>=5, 100>=N>=10. The composite organic nitrogen sources include microorganism sources, plant sources and animal sources. The quantitative indexes include natural amino acid, vitamins, inorganic salt, saccharides, nucleic acid and the like, and the number of the quantitative indexes is far larger than that of indexes provided by an existing composite organic nitrogen source manufacturing enterprise. Therefore, the necessity that research and production staff seek for composite organic nitrogen source suppliers everywhere is reduced, time cost is saved, and the range of screened samples is widened; besides, indexes in the kit can be used as the raw material standard, controllability and stability of the process can be improved, and the reproducibility of research and production results can be improved.

Description

technical field [0001] The invention belongs to the technical field of biological culture raw materials, and in particular relates to a composite organic nitrogen source kit. Background technique [0002] Biological growth is inseparable from nitrogen sources, carbon sources and other micronutrients. Nitrogen source is an important factor affecting the growth, reproduction and metabolism of organisms. Nitrogen sources can be divided into inorganic nitrogen sources and organic nitrogen sources (also called composite nitrogen sources, or composite organic nitrogen sources). Compound organic nitrogen source is an indispensable raw material in the field of biological cultivation. According to the source, it can be divided into nitrogen source derived from microorganisms, such as yeast powder; nitrogen source derived from animals, such as fish meal and bovine extract; nitrogen source derived from plants, such as peanut cake powder , corn syrup, etc. [0003] During the biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P19/62C12P35/00C12R1/465C12R1/75
CPCC12N1/14C12N1/20C12P19/62C12P35/00
Inventor 王晓雨
Owner 王晓雨