Molecular marker SVhd1 closely linked to growth-period gene of millet

A technology of millet growth period and molecular markers, applied in the field of molecular biology, can solve the problem of gene mapping research that has not been reported in the literature

Inactive Publication Date: 2017-07-04
SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature report on the mapping of genes related to the growth period of millet.

Method used

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  • Molecular marker SVhd1 closely linked to growth-period gene of millet
  • Molecular marker SVhd1 closely linked to growth-period gene of millet
  • Molecular marker SVhd1 closely linked to growth-period gene of millet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Construction of millet RIL population

[0035] Male parent: Ningxia Zaogu, precocious (short growth period, 104 days). Female parent: Wudang 2 (WD-2), late maturing (long growth period, 116 days). The male parent and the female parent were crossed to obtain F1, and the F1 generation obtained 215 recombinant inbred lines RIL strains (F6 generation) through the single-grain method. Ningxia Zaogu and WD-2 can be purchased from Shenzhen Huada Agriculture and Circular Economy Technology Co., Ltd.

Embodiment 2

[0036] Example 2: Extraction of Genomic DNA

[0037] For the millet RIL population obtained in Example 1, use the CTAB method to extract the genomic DNA of the parents and the individual plants of the RIL population respectively. The specific methods are as follows:

[0038] (1) Weigh 1.0g of fresh leaves, cut them into pieces and put them in a mortar, grind them with liquid nitrogen, add 3mL 1.5×CTAB, grind them into a homogenate and transfer them to a 15mL centrifuge tube, then add 1mL 1.5×CTAB into the mortar Rinse and transfer to a centrifuge tube. After mixing, place in a water bath at 65°C for 30 minutes, and shake slowly from time to time during this period.

[0039]Among them, the formula of 1.5×CTAB is as follows (1L):

[0040] CTAB 15g 1mol / L Tris.Cl (pH 8.0) 75mL 0.5mol / L of EDTA 30mL NaCl 61.4g

[0041] Add deionized water to make up to 1 L, and add mercaptoethanol with a final concentration of 0.2% (2 ml) before use.

[0042]...

Embodiment 3

[0047] Example 3: Gene Mapping and Molecular Marker Development

[0048] (1) Genetic map construction

[0049] For the genomic DNA of the RIL individual obtained in Example 2, RAD-seq-based genotyping technology (http: / / www.bioon.com.cn / server / show_product.asp?id=12291) was used for individual RIL population Genotyping was performed to obtain genotype data for the RIL population.

[0050] Use MapMaker 3.0 software (Constructing genetic maps with MAPMAKER / EXP 3.0, S Lincoln, M Daly, E Lander-Cambridge, MA: Whitehead Institute, 1992, which is incorporated herein by reference) to draw a genetic linkage map to obtain a genetic linkage map .

[0051] (2) Gene mapping

[0052] For the RIL population obtained in Example 1, the individual phenotypes of the RIL population, which are similar to the paternal traits, are marked as a, those similar to the maternal traits are marked as b, and the traits are between the male parent and the female parent Denote it as h. Obtain the phenot...

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Abstract

The invention relates to a molecular marker, specifically to a molecular marker closely linked to the growth-period gene of millet, belonging to the field of molecular biology. The molecular marker has a nucleotide sequence as shown in SEQ ID No. 1; or the molecular marker is a DNA fragment, containing the nucleotide sequence as shown in SEQ ID No. 1, from the genome of millet. The invention also relates to primers of the molecular marker, application of the molecular marker to positioning of the growth-period gene of millet or to genetic breeding of millet, a positioning method for the growth-period gene of millet, and a breeding method for millet. According to the invention, the molecular marker SVhd1 closely linked to the growth-period gene of millet is discovered; and combination of the genomic DNA sequence of millet with the growth-period gene of millet is more favorable for establishment of a molecular marker-assisted breeding system for millet.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a molecular marker closely linked with a millet growth period gene. The present invention also relates to the primer of the molecular marker, the use of the molecular marker in gene mapping of millet growth period or millet genetic breeding, a method of gene mapping of millet growth period, and a method of millet breeding. Background technique [0002] Millet originated in China and is a traditional dominant crop, staple food crop and drought-resistant and barren-resistant crop. Millet is resistant to drought and barrenness, has high water use efficiency and wide adaptability. It not only plays an important role in the current dry farming ecological agriculture, but also is an important strategic reserve crop for the increasingly serious water shortage. The millet after millet hulling is rich in nutrients and balanced in various components. It i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6895C12Q1/6806C12Q2600/13C12Q2531/113
Inventor 张耕耘周承恕李宁雷雪静刘小菊
Owner SHENZHEN BGI AGRI & CYCLE ECONOMIC TECH
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