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Method for culturing root apex of butterfly orchid sterile culture strain

A technique of moth orchid tissue culture, applied in the field of root tissue culture of Phalaenopsis aseptic culture strains, can solve the problems of high degree of lignification, poor ability to form root system, low tissue culture multiplication coefficient, etc., to reduce damage, reduce The degree of pollution, the effect of obvious advantages

Inactive Publication Date: 2017-07-14
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The conventional tissue culture method of Phalaenopsis is to generally use the flower stem of the plant as the explant for tissue culture, which is easy to be polluted, the number of tissue culture generations should not be too many, and the sampling is limited, and the tissue culture proliferation coefficient is low
And the degree of lignification of the flower stem is high, the activity of the parenchyma cells is poor, and the ability to form callus or form a root system as an explant is poor

Method used

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  • Method for culturing root apex of butterfly orchid sterile culture strain
  • Method for culturing root apex of butterfly orchid sterile culture strain
  • Method for culturing root apex of butterfly orchid sterile culture strain

Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) adopt finely crushed Chinese fir dander to cultivate the Phalaenopsis sterile strain, and the sterile strain is placed in an independent climate box; the tip 1 cm of the aerial root protruding from the outside of the flower pot is cut off with a double-sided blade as an explant;

[0020] (2) Wash the root tip of the explant with washing powder for 10 minutes, soak it in alcohol with a volume fraction of 70% for 30 seconds, then soak it in 0.5% sodium hydrochloride solution for 5 minutes to sterilize, and finally rinse it with sterilized water for 3 times;

[0021] (3) Insert the root tip of the washed explant into the medium for tissue culture and culture for one month. The medium is: 1 / 3MS+1mg / L BA+0.01mg / L NAA, including The mass ratio is 0.2% gellan gum, and the mass ratio is 0.5% sucrose; the culture conditions are temperature 25° C., light 3600 lx, and light 12 hours per day.

[0022] After one month of cultivation in Example 1, the tissue culture differentiati...

Embodiment 2

[0024] (1) Adopt finely crushed Chinese fir dander to cultivate Phalaenopsis aseptic strains, and the aseptic strains are placed in an independent climate chamber; the tip 1.5 cm of aerial roots stretching out of the flowerpot is cut off with a double-sided blade as an explant;

[0025] (2) Wash the root tip of the explant with washing powder for 12 minutes, soak it in alcohol with a volume fraction of 70% for 45 seconds, then soak it in 0.5% sodium hydrochloride solution for 6 minutes to sterilize, and finally rinse it with sterilized water for 4 times;

[0026] (3) Insert the root tip of the washed explant into the medium for tissue culture and culture for one month. The medium is: 1 / 3MS+1mg / L BA+0.01mg / L NAA, including The mass ratio is 0.2% gellan gum, and the mass ratio is 0.5% sucrose; the culture conditions are temperature 25° C., light 3600 lx, and light 12 hours per day.

[0027] After one month of cultivation in Example 2, the tissue culture differentiation rate at t...

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Abstract

The invention discloses a root tip tissue culture method of Phalaenopsis aseptic culture strain, which comprises the following steps: (1) cultivating Phalaenopsis aseptic strain with finely divided Chinese fir dander, cutting off the tips of aerial roots protruding from the flower pot as explant; (2) washing the root tip of the explant with washing powder, soaking it in alcohol, soaking it in sodium hydrochloride to sterilize it, and rinsing it with sterilized water at last; (3) washing the root tip of the explant Into the culture medium for tissue culture. The method adopts the root of the aseptic cultured strain as the explant, which can effectively reduce the pollution degree of the explant, reduce the damage to the mother plant, and promote the differentiation rate and proliferation rate of the root tip tissue culture. The invention can collect root ends in any season for tissue culture, and the explants can be collected without waiting for the Phalaenopsis to bloom.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a root tip tissue culture method of Phalaenopsis aseptic culture strains. Background technique [0002] Phalaenopsis aphrodite Rchb.F. belongs to Orchidaceae Phalaenopsis, originates in subtropical rainforest area, and is an epiphytic orchid. The thick white aerial roots of Phalaenopsis are exposed around the leaves. In addition to absorbing nutrients in the air, they also have growth and photosynthesis. In the Spring Festival, the Phalaenopsis plant has long flower stalks from the leaf axils and blooms like butterflies flying. It is favored by flower lovers and is known as the "Queen of the Orchid". Distributed in Thailand, Philippines, Malaysia, Indonesia, and Taiwan. [0003] The conventional tissue culture method of Phalaenopsis is to generally use the flower stem of the plant as an explant for tissue culture, which is easy to be polluted, the number of generations of tissue culture ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 周余华周琴裴新国席映雪
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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