Preparation method of strawberry extract for cigarettes
A technology for strawberry extract and cigarettes, which is applied in the direction of producing fat, essential oil/flavor, etc., can solve the problems that have not been reported, and achieve the effect of reducing production cost, good flavor quality, and simple process
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Embodiment 1
[0018] (1) Wash the strawberries, remove the skin, put them into a cold press for cold pressing, and take out the juice to obtain strawberry juice;
[0019] (2) Dissolve 0.2g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 18mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 26mL Calcium chloride solution with a mass concentration of 2%, embedded at 4°C for 1.5h, and washed with buffer solution to obtain sodium alginate-immobilized enzyme with a loading capacity of 15U / g;
[0020] (3) Treat strawberry juice with immobilized β-glucosidase, the solid dosage of immobilized β-glucosidase is 15g / L, the pH value of enzyme treatment is 6, the temperature of enzyme treatment is 35°C, and the time of enzyme treatment is 3.5h. Subsidence treatment at 2°C for 36 hours;
[0021] (4) Filter the enzyme-treated extract with filter paper to remove large particles of impurities to obtain a clarified strawberry extract. Concentrat...
Embodiment 2
[0024] (1) Wash the strawberries, remove the skin, put them into a cold press for cold pressing, and take out the juice to obtain strawberry juice;
[0025] (2) Dissolve 0.15g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 15mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 20mL The calcium chloride solution with a mass concentration of 2% was embedded at 4° C. for 2 hours, and then washed with a buffer solution to obtain immobilized β-glucosidase with a loading capacity of 10 U / g.
[0026] (3) Take immobilized β-glucosidase to treat strawberry juice, the solid dosage of immobilized β-glucosidase is 26g / L, the pH value of enzyme treatment is 7, the temperature of enzyme treatment is 38°C, and the time of enzyme treatment is 4.2h. Subsidence treatment at 3°C for 40h;
[0027] (4) Filter the enzyme-treated extract with filter paper to remove large particles of impurities to obtain a clarified strawberry extract...
Embodiment 3
[0030] (1) Wash the strawberries, remove the skin, put them into a cold press for cold pressing, and take out the juice to obtain strawberry juice;
[0031] (2) Dissolve 0.3g of solid β-glucosidase in 1mL of citric acid buffer (0.1mol / L, pH4.5), add 20mL of sodium alginate solution with a mass concentration of 2%, stir well, and then add 30mL Calcium chloride solution with a mass concentration of 2%, embedded at 4°C for 3 hours, and washed with buffer solution to obtain immobilized β-glucosidase with a loading capacity of 20 U / g.
[0032] (3) Take immobilized β-glucosidase to treat strawberry juice, the solid dosage of immobilized β-glucosidase is 38g / L, the pH value of enzyme treatment is 8, the enzyme treatment temperature is 43°C, and the enzyme treatment time is 4.8h. After the treatment is completed Subsidence treatment at 5°C for 48 hours;
[0033] (4) Filter the enzyme-treated extract with filter paper to remove large particles of impurities to obtain a clarified straw...
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