Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus licheniformis strain TA65 and application thereof in promotion of thorough decomposing of compost

A technology of Bacillus licheniformis, TA65, applied in the application, preparation of organic fertilizer, bacteria and other directions, to achieve the effect of promoting compost decomposing, fast growth and reproduction, and increasing compost temperature

Active Publication Date: 2017-07-18
GUANGXI UNIV
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, Bacillus licheniformis is mainly used as an animal feed additive, but there is no relevant report on the application of Bacillus licheniformis in promoting compost maturity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus licheniformis strain TA65 and application thereof in promotion of thorough decomposing of compost
  • Bacillus licheniformis strain TA65 and application thereof in promotion of thorough decomposing of compost
  • Bacillus licheniformis strain TA65 and application thereof in promotion of thorough decomposing of compost

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 : Screening of Bacillus licheniformis A65 (Bacillus licheniformis)

[0040] 1. Experimental materials and equipment

[0041] 1.1 Main reagents

[0042] Table 1 Main Reagents

[0043]

[0044]

[0045] 1.2 Main instruments and equipment

[0046] Table 2 Main instruments and equipment

[0047] equipment name model Manufacturer UV-visible spectrophotometer UV759 Shanghai Jingke clean bench SW-CJ-1F Suzhou Antai Air Technology Co., Ltd. Automatic Balancing Centrifuge PSZ4-1.2 Beijing Medical Centrifuge Factory Rayro pH Meter PHS-2S Shanghai Yidian Instrument Co., Ltd. Constant temperature shaker HQ45Z Wuhan Zhongke Technology Development Co., Ltd. Biochemical incubator SPX-150B Shanghai Yuejin Medical Instrument Factory Electronic thermostat universal electric furnace DK-98-II Tianjin Test Instrument Co., Ltd. refrigerator Electronic balance YP6102 Shanghai Gua...

Embodiment 2

[0140] Example 2 : Identification of Bacillus licheniformis A65 (Bacillus licheniformis)

[0141] 2.1 The main instruments of the experiment

[0142] Table 4 Instruments used in experiments

[0143]

[0144] 2.2 Experimental steps

[0145] 2.2.1 DNA extraction

[0146] DNA was extracted using Sangon's "Ezup Column Bacterial Genomic DNA Extraction Kit Instructions". Please refer to the manual for specific operation steps.

[0147] 2.2.2 PCR amplification

[0148] Use 2×Es Taq MasterMix for PCR amplification, and the reaction system is as follows:

[0149]

[0150] Reaction procedure:

[0151]

[0152] 2.2.3 Electrophoresis results of PCR products

[0153] Use 1% concentration of agarose gel electrophoresis, voltage 120V, electrophoresis 30min, load 1uL per hole, see the electrophoresis pattern Figure 13 .

[0154] Such as Figure 13 As shown, the results of PCR product electrophoresis indicated that the bacterial 16S rDNA fragment was successfully amplifie...

Embodiment 3

[0161] Example 3 : Application of a bacterial agent containing Bacillus licheniformis strain TA65 in promoting compost maturity

[0162] 3.1 Bacteria preparation

[0163] Inoculate the TA65 seed solution into 1L of sterilized LB culture solution at an inoculum amount of 1% (v / v), culture it in a shaker (50°C, 120rmp) for 24 hours, and collect the culture solution as the bacterial agent. The bacteria-adding group was the experimental group (T), and the blank group (CK) was added with 1 L of sterilized LB culture solution.

[0164] 3.2 Composting experiment

[0165] Cow dung and sugarcane leaves are selected as compost raw materials, with a mass ratio of 17:3, totaling 20kg. After composting for 45 days, samples were taken at 0, 5, 10, 16, 23, 30, and 45 days, and parameters such as temperature, pH, moisture content, organic matter, Kjeldahl nitrogen, inorganic nitrogen, and DOM content were measured. The whole composting process was inoculated with bacteria twice, respecti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of development of plant growth-promoting bacteria and particularly relates to a bacillus licheniformis strain TA65 and application thereof in promotion of thorough decomposing of compost. The bacillus licheniformis strain TA65 is chemically named as bacillus licheniformis TA65, is preserved on January 5, 2017 in China General Microbiological Culture Collection Center and has the preservation number of CGMCC 13531. The bacillus licheniformis strain TA65 is sieved and separated from a high-temperature stage compost sample for the first time and can resist high temperature, rapidly grow and propagate and produce lignocellulose degrading enzyme; by utilizing a bacterial agent containing the bacillus licheniformis strain TA65, the compositing temperature can be increased, the degrading of organic matters and dissolved organic matters (DOM) can be accelerated, the content of Kjeldahl nitrogen can be increased, and the thorough decomposing of the compost can be promoted; and the bacillus licheniformis strain TA65 further has a function of producing a biological surfactant, so that the strain can be applied to preparation of surfactants.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria development, and specifically relates to a bacillus licheniformis strain TA65 and its application in promoting compost decomposing. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production. However, with the increase in the use of chemical fertilizers, its utilization rate has decreased year by year. Residues of pesticides and chemical fertilizers have released a large amount of harmful substances to the environment, polluting soil, water sources and food, and posing a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top priority. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N9/02C12N9/08C12N9/24C12N9/42C05F11/08C05F17/00C12R1/10
CPCC05F11/08C05F17/00C12N1/20C12N9/0061C12N9/0065C12N9/2437C12N9/2477C12Y110/03002C12Y111/01013C12Y111/01014C12N1/205C12R2001/10Y02W30/40
Inventor 李群良陆彦宇徐佳琦
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com