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Method for separating high-purity oleuropein by combining macroporous resin and preparative liquid chromatography

A technology for preparing liquid phase and oleuropein, which is applied in the preparation of sugar derivatives, chemical instruments and methods, organic chemistry, etc., can solve the problems of high solvent consumption, cumbersome steps, low yield and the like, and achieves low separation cost and low preparation cost. The effect of process safety

Active Publication Date: 2017-07-21
云南油橄榄大健康产业创新研究发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the purity of the compound obtained in this patent is high, the steps are cumbersome, the cycle is long, the solvent consumption is large, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 1kg of olive leaves, add 12 liters of 60% ethanol, reflux for 2 hours, pour out the extract, then add 10 times the amount of 60% ethanol for the second extraction, combine the two extracts, and then recover the solvent under reduced pressure until there is no alcohol smell in the solution. Add distilled water to the concentrated extract to make the density equivalent to 0.2g / mL of the original medicinal material. The concentrated extract was passed through a macroporous resin column to enrich oleuropein at a flow rate of 8 BV / h. After the adsorption was completed, it was first eluted with distilled water, and the distilled water eluate was discarded. Then elute with 70% ethanol at a flow rate of 10BV / h, collect the 70% ethanol eluate, and recover the solvent under reduced pressure. Dissolve the 70% ethanol eluted part of the macroporous resin in methanol, and separate by preparative liquid chromatography, using a 20×250mm separation column, with a sample volume of...

Embodiment 2

[0021] Take 1 kg of olive leaves, add 12 liters of water, reflux for 2 hours, pour out the extract, then add 10 times the amount of water for the second extraction, combine the two extracts, and recover the solvent under reduced pressure until the density of the extract is equivalent to The original medicinal material 0.15g / mL. The concentrated extract was passed through a macroporous resin column for oleuropein enrichment at a flow rate of 10BV / h. After the adsorption was completed, the impurities were first washed with distilled water, and the distilled water eluate was discarded. Then elute with 60% ethanol at a flow rate of 10BV / h, collect the 60% ethanol eluate, and recover the solvent under reduced pressure. Dissolve the eluted part of the macroporous resin with 60% ethanol in methanol, and separate by preparative liquid chromatography, using a 50×650mm preparative column, with a sample volume of 150mg, methanol-water gradient elution, a flow rate of 30mL / min, and an onl...

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Abstract

The invention relates to a method for separating high-purity oleuropein by combining macroporous resin and preparative liquid chromatography. By adopting the method, an oleuropein compound having the purity of 90% or above can be obtained. The separation cost of the method is low, the preparation process is safe, the oleuropein having the purity of 90% or above can be rapidly separated and prepared from an olive leaf extract by combining the macroporous resin and the preparative liquid chromatography, and the whole separating method is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and relates to a method for separating high-purity oleuropein by using a macroporous resin combined with a liquid chromatography technology. Background technique [0002] Oleuropein is an iridoid glycoside compound, which mainly exists in the fruit and leaves of the olive tree, and the content in the leaves is the highest. Because oleuropein contains multiple phenolic hydroxyl groups, it has strong antioxidant capacity, can prevent the occurrence of coronary heart disease and atherosclerosis, relieve vascular smooth muscle, and reduce the oxidation of low-density lipoprotein. In addition, oleuropein also has Good anti-inflammatory, anti-fungal, anti-viral and hypoglycemic activities. Oleuropein is also used as an additive in a variety of skin care products, mainly to maintain skin elasticity, prevent skin aging, and resist skin cell damage caused by oxidation. [0003] In recent years, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
CPCC07H1/08C07H17/04
Inventor 黄新异邸多隆
Owner 云南油橄榄大健康产业创新研究发展有限公司
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