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Nano-pore detection system based on nano-tube and preparation method and application thereof

A detection system and nanotube technology, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of poor mechanical stability of two-dimensional material films, reduced signal-to-noise ratio, low signal-to-noise ratio, etc., and achieve good results Effects of Mechanical Stability, Improved Resolution and Sensitivity

Active Publication Date: 2017-07-21
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

First, the mechanical stability of 2D material films is poor
The two-dimensional material film with sub-nanometer thickness is prone to mechanical vibration in the electrolyte solution, and the vibration noise generated will make the weak signal generated by the ionic current submerged in the noise and cannot be recognized; second, two-dimensional materials such as graphene are transferred to its support. Defects will be generated in the substrate process, and the leakage current generated by the defects is also considered to be one of the reasons for the low signal-to-noise ratio of the current signal detected by the nanopores of two-dimensional materials.
Third, compared with traditional silicon-based nanopores, although the atomic-level channel length of two-dimensional material nanopores can improve the spatial resolution of current signals, it also allows DNA molecules to maintain a greater degree of freedom when passing through the pores. This leads to an unstable molecular configuration and reduces the signal-to-noise ratio
[0004] Most of the existing nanotube biosensors based on the nanopore detection principle integrate nanotubes into silicon-based nanopores (CN103796947B) or use radial slices of nanotubes to form nanopores (CN 1994864B), but the nanotubes formed by these methods hole, there are still problems of low spatial resolution and low signal-to-noise ratio

Method used

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  • Nano-pore detection system based on nano-tube and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] This embodiment provides a nanopore detection system based on single-walled carbon nanotubes. The specific implementation is as follows:

[0045] Grow nanotubes 14 with a diameter of 1-10nm and a length of 500-1000μm on the surface of the substrate 5 with a silicon dioxide film. The negative photoresist is spin-coated on the substrate 5 with the nanotubes 14, and light is used. The method of engraving is to process a negative photoresist micro-channel structure 6 with a thickness of 20 μm and a width of 7 μm on the selected nanotube 14 with a diameter of 2 nm and a length of 500 μm to form a first cavity 11 and a second cavity Chamber 12 and third chamber 13.

[0046] The first chamber 11 and the second chamber 12, the second chamber 12 and the third chamber 13 are not connected, and the first chamber 11 and the third chamber 13 are only connected by nanotubes 14. The two ends of the nanotube 14 are processed with a focused ion beam to ensure that the two ends of the nanot...

Embodiment 2

[0051] This embodiment provides a nanopore detection system based on boron nitride nanotubes. The specific implementation is as follows:

[0052] Disperse nanotubes 14 with a diameter of 300-500nm and a length of 10-90μm on the surface of the substrate 5, spin-coating positive photoresist on the substrate with nanotubes, and use photolithographic processing to set the selected diameter A positive photoresist micro-channel structure 6 with a thickness of 15 μm and a width of 8 μm is processed on a nanotube 14 of 400 nm and a length of 20 μm to form a first cavity 11, a second cavity 12 and a third cavity 13.

[0053] The first chamber 11 and the second chamber 12, the second chamber 12 and the third chamber 13 are not connected, and the first chamber 11 and the third chamber 13 are only connected by selected nanotubes 14 . The two ends of the nanotube 14 are processed with a focused ion beam to ensure that the two ends of the nanotube 14 are not blocked. A focused ion beam is use...

Embodiment 3

[0058] This embodiment provides a nanopore detection system based on multi-wall carbon nanotubes. The specific implementation is as follows:

[0059] Grow nanotubes with a diameter of 10-90nm and a length of 100-500μm on the surface of the substrate 5, spin-coating a negative photoresist on the substrate 5 with nanotubes, and use a photolithography method to select A negative photoresist micro-channel structure 6 with a thickness of 25 μm and a width of 5 μm is processed on the nanotube 14 to form a first cavity 11, a second cavity 12 and a third cavity 13.

[0060] The first chamber 11 and the second chamber 12, the second chamber 12 and the third chamber 13 are not connected, and the first chamber 11 and the third chamber 13 are only connected by nanotubes 14. The two ends of the nanotube 14 are processed with a focused ion beam to ensure that the two ends of the nanotube 14 are not blocked. A transmission electron microscope is used to process the part of the nanotube 14 expos...

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Abstract

The invention discloses a nano-pore detection system based on a nano-tube and a preparation method and application thereof. The system comprises a nano-pore structure based on the nano-tube, a micro-channel structure, a cover plate for sealing the micro-channel structure, and a current detection system for detecting a to-be-detected object. The nano-pore structure based on the nano-tube comprises a substrate and the nano-tube. The nano-tube is dispersed or grows on the substrate, openings are formed in the left end and the right end of the nano-tube, and a nano-pore is formed in the side wall. The micro-channel structure is machined on the substrate and provided with three independent electrolyte solution cavities, the nano-tube penetrates through the three cavities, and the left opening, the nano-pore and the right opening of the nano-tube are communicated with the first cavity, the second cavity and the third cavity respectively. One end of the current detection system is arranged in the second cavity, and the other end of the current detection system is arranged in the first cavity or the third cavity. The resolution and sensitivity of a bio-sensor can be greatly improved, and the concept of single molecule DNA direct sequencing is expected to be achieved.

Description

Technical field [0001] The invention relates to the technical field of nanopore detection systems, in particular to a nanopore detection system based on a nanotube structure that can be used to detect long-chain polymers (DNA, RNA or polypeptide) and a preparation method thereof. Background technique [0002] Nanopore technology is a powerful tool for the analysis of biomolecules, and has been specifically applied to the following three areas: First, biophysical research at the single-molecule level, such as the folding / unfolding of DNA molecules, the melting process of double-stranded DNA, Single-molecule DNA-protein interaction and force spectroscopy measurement of biological molecules, etc.; second, a broad "early diagnosis" at the single-molecule level. Such as the identification of important attributes of a single biomolecule, the distinction of specific short fragments, etc.; third, the direct sequencing of single molecule DNA. [0003] As the first nanopore to be applied to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 王赟姣王德强刘梦婉邓云生袁家虎
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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