Supercharge Your Innovation With Domain-Expert AI Agents!

An anti-dopamine monoclonal antibody hybridoma cell line gz01 and its application

A hybridoma cell line, dopamine single technology, applied in instruments, peptides, immunoglobulins, etc., can solve the problems of high cost, time-consuming, inability to achieve rapid sample detection, and achieve good specificity.

Active Publication Date: 2020-01-10
JIANGNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting β-adrenergic receptor stimulants are mainly gas chromatography (GC), liquid chromatography (HPLC), spectrophotometry, molecular imprinting, capillary electrophoresis, gas chromatography, electrochemical immunosensor and other methods. However, these methods have disadvantages such as cumbersome operation, time-consuming, and expensive costs, and cannot realize rapid detection of a large number of samples. Therefore, it is of great significance to establish a fast and simple detection method.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An anti-dopamine monoclonal antibody hybridoma cell line gz01 and its application
  • An anti-dopamine monoclonal antibody hybridoma cell line gz01 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of hybridoma cell line GZ01

[0022] 1. Synthesis of complete antigen

[0023] Weigh 2.57 mg of dopamine and add 200 μL of 25-fold diluted glutaraldehyde solution to a brown bottle, and react at 4°C for 30 min to obtain an activation solution; weigh 10 mg of protein BSA and dissolve it in 2 mL of carbonate buffer solution CBS, and Add the activation solution dropwise to the protein solution, adjust the pH to 9.0 while adding the activation solution dropwise, stop adding the activation solution until the color of the reaction solution turns yellow, react at 4°C for 30 min to obtain the coupling product, and separate the complete antigen and untreated protein by dialysis. Coupled small molecules and identified by UV spectrophotometry.

[0024] 2. Animal immunity

[0025] Healthy BALB / c mice aged 6-8 weeks were selected for immunization. The dopamine complete antigen was mixed and emulsified with an equal amount of Freund's adjuvant, and BALB / c mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses an anti-dopamine monoclonal antibody hybridoma cell strain GZ01 and application thereof, and belongs to the field of food safety immunoassay. The anti-dopamine monoclonal antibody hybridoma cell strain GZ01 is collected in China General Microbiological Culture Collection Center (CGMCC), and the collection number is CGMCC No. 13089. A monoclonal antibody secreted by the cell strain has high specificity and detection sensitivity on dopamine, the IC50 value is 531ng / mL, and the cell strain can be applied to detection of dopamine residues in food.

Description

technical field [0001] The invention discloses an anti-dopamine monoclonal antibody hybridoma cell line GZ01 and its application, which belong to the field of food safety immune detection. Background technique [0002] β-adrenoreceptor stimulants are a class of feed additives that can promote the growth of lean meat, including clenbuterol hydrochloride, brobuterol, mabuterol, salbutamol, ractopamine, dopamine hydrochloride, terbutaline sulfate, 15 drugs including phenylethanolamine A, bambuterol, zilpaterol hydrochloride, clorprenaline hydrochloride, sibuterol, arformoterol tartrate, and formoterol fumarate. Adding β-adrenergic receptor stimulants to feed can significantly increase the lean meat growth rate of pigs, cattle, sheep and other livestock, so that the body weight can also be increased. This type of drug can be ingested through eating, endangering human health, and causing obvious physiological toxicity, such as palpitations, trembling, nausea and vomiting, dizzin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/44G01N33/577G01N33/94
CPCC07K16/44G01N33/577G01N33/9413
Inventor 刘丽强刘睿胥传来匡华徐丽广马伟吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com