Method for preparing moringa leaf protein polypeptide through microwave assisting and membrane filtration

A microwave-assisted, protein-peptide technology, applied in the field of protein-peptide, can solve the problems of long time, difficult digestion and absorption, and high efficiency, and achieve the effects of fast digestion and absorption, high protein utilization rate, and low viscosity.

Inactive Publication Date: 2017-07-28
广西肽王生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method for preparing Moringa leaf protein polypeptide in the industry takes a long time, has high efficacy, and is not easy to be digested and absorbed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing Moringa oleifera leaf protein polypeptide with microwave assistance and membrane filtration, comprising the following steps:

[0022] (1) After washing the fresh Moringa leaves, dry them naturally for later use;

[0023] (2) Crush the washed Moringa leaves to a 15-mesh sieve;

[0024] (3) Take qualified pulverized Moringa leaf powder and add pure water according to the solid-to-liquid ratio of 1:15, adjust the pH value to 8.5, heat to 55°C in a microwave oven, and keep the temperature for 1 hour;

[0025] (4) After the Moringa leaf powder solution treated above is cooled to room temperature and the pH value is adjusted to 8.5 again, alkaline protease is added at 3% of the solution weight, pectinase is added at 0.002% of the total weight, and fully stirred. Start the microwave heating again for 10 minutes, and control the temperature at 55°C. During this process, keep the pH value at 7.5-8 to obtain the first enzymatic hydrolysis solution;

[002...

Embodiment 2

[0029] A method for preparing Moringa oleifera leaf protein polypeptide with microwave assistance and membrane filtration, comprising the following steps:

[0030] (1) After washing the fresh Moringa leaves, dry them naturally for later use;

[0031] (2) Crush the washed Moringa leaves to a 20-mesh sieve;

[0032] (3) Take qualified pulverized Moringa leaf powder and add pure water according to the solid-to-liquid ratio of 1:15, adjust the pH value to 8.5, heat to 55°C in a microwave oven, and keep the temperature for 1 hour;

[0033] (4) After the Moringa leaf powder solution treated above is cooled to room temperature and the pH value is adjusted to 8.5 again, alkaline protease is added at 3% of the solution weight, pectinase is added at 0.002% of the total weight, and fully stirred. Start the microwave heating again for 15 minutes, and control the temperature at 55°C. During this process, keep the pH value at 7.5 to obtain the first enzymatic hydrolysis solution;

[0034]...

Embodiment 3

[0037] A method for preparing Moringa oleifera leaf protein polypeptide with microwave assistance and membrane filtration, comprising the following steps:

[0038] (1) After washing the fresh Moringa leaves, dry them naturally for later use;

[0039] (2) Pulverize the washed leaves of Moringa oleifera into a 50-mesh sieve;

[0040] (3) Take qualified pulverized Moringa leaf powder and add pure water according to the solid-to-liquid ratio of 1:15, adjust the pH value to 8.5, heat to 55°C in a microwave oven, and keep the temperature for 1 hour;

[0041] (4) After the Moringa leaf powder solution treated above is cooled to room temperature and the pH value is adjusted to 8.5 again, alkaline protease is added at 3% of the solution weight, pectinase is added at 0.002% of the total weight, and fully stirred. Start the microwave heating again for 15 minutes, and control the temperature at 55°C. During this process, keep the pH value at 8 to obtain the first enzymatic hydrolysis solut...

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PUM

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Abstract

The invention relates to a method for preparing a moringa leaf protein polypeptide through microwave assisting and membrane filtration. The method comprises: crushing moringa leaf, adding alkaline protease and pectinase, carrying out enzymolysis, and filtering with a ceramic membrane and a polysulfone membrane to obtain the moringa leaf protein polypeptide. According to the present invention, by using the microwave heating, the solubility of the protein in the weak alkaline solution is promoted, the extraction rate of the protein is 47.35%, and the extraction rate of the moringa leaf protein is increased; and by using the two-step filtration with the ceramic membrane and the polysulfone membrane, impurities, bacteria and small particle substances are completely removed from the solution so as to ensure the safety of the moringa leaf protein polypeptide.

Description

technical field [0001] The invention belongs to the field of protein polypeptides, in particular to a method for preparing moringa leaf protein polypeptides with microwave assistance and membrane filtration. Background technique [0002] The active polypeptide obtained by enzymatic hydrolysis of the protein in Moringa oleifera leaves is one of the most active fields of research on functional plant leaf protein polypeptides. However, the current method for preparing Moringa leaf protein polypeptide in the industry takes a long time, has high efficacy, and is not easy to be digested and absorbed. [0003] In view of the above-mentioned shortcomings, the purpose of the technical innovation of the present invention is to improve the enzymatic hydrolysis process and technology of Moringa leaf protein, solve the problem that Moringa leaf protein is not easy to be digested and absorbed by animals, and prepare biologically active oligosaccharides by using microwave-assisted enzymati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14A23J3/14A23J3/34
CPCA23J3/14A23J3/34A23J3/347A23V2002/00C07K1/145C07K1/34C12P21/06A23V2250/55
Inventor 雷少玲梁尚文梁满水施雷浩茗
Owner 广西肽王生物科技有限公司
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