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Method for extracting isoflavone from soybean

A technology for isoflavones and isoflavone glycosides is applied in the field of high-purity isoflavone products, which can solve the problems of unsuitable full-fat soybean powder extraction, undiscovered medicinal value, and methanol harmful to human health, etc., and achieves easy regeneration and simple operation. , the effect of high extraction rate

Inactive Publication Date: 2001-08-22
INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] 1. The extraction of full-fat soybean powder is not suitable for the use of lye;
[0008] 2. When precipitating protein, some isoflavones are taken away, and the recovery rate is low;
[0009] 3. Use methanol to elute during column chromatography, and residual methanol is harmful to human health;
[0010] 4. More than half of the product is malonyl isoflavone, and the relevant research reports at home and abroad retrieved by the inventor have not yet found its medicinal value

Method used

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  • Method for extracting isoflavone from soybean
  • Method for extracting isoflavone from soybean
  • Method for extracting isoflavone from soybean

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 technological process is with reference to appendix figure 1 .

[0071] The present invention uses 1000 grams of soybean flour (full-fat soybean flour), puts it into a 5000ml Soxhlet fat extractor after packing it with a filter cloth bag, and takes out the filter cloth bag after refluxing with 5000ml n-hexane on a 75° C. water bath for 8 hours, and waves to remove the residue The normal hexane is the defatted soybean meal.

[0072] Weigh 1000 grams of defatted soybean powder, put it into a 10000ml flask, add 6000ml of 70% alcohol and 0.3% acetic acid mixed extract, stir and extract at room temperature for 2 hours, then filter, and wash the filter residue three times with a small amount of new extract. The filtrate was concentrated under reduced pressure at 65°C to 1000ml, and then hydrolyzed in a water bath at 80°C for 15 hours (the first step of hydrolysis), and all the malonyl isoflavone glycosides were hydrolyzed into isoflavone glycosides. After the h...

Embodiment 2

[0081] Embodiment 2 technological process is with reference to appendix figure 2

[0082] Weigh 1000 grams of defatted soybean powder, put it into a 10000ml flask, add 6000ml of 70% alcohol and 0.3% acetic acid mixed extract, stir and extract at room temperature for 2 hours and then filter. The filter residue was washed three times with a small amount of new extract, and the filtrate was concentrated under reduced pressure at 65°C to 1000ml, and then hydrolyzed in a water bath at 80°C for 15 hours (the first step of hydrolysis), and all malonyl isoflavone glycosides were hydrolyzed into isoflavone glycosides. After the hydrolyzate is cooled, it passes through a φ3.0×50cm polyamide chromatography column at a flow rate of 5-10ml / min. After the hydrolyzate has run out, rinse the column with water until the effluent is a colorless transparent liquid, and stop rinsing. The chromatography column was eluted with 500ml (about twice the column volume) of 70% alcohol. The received 5...

Embodiment 3

[0085] Embodiment 3 technological process is with reference to appendix image 3 .

[0086]Weigh 1000 grams of defatted soybean powder, put it into a 10000ml flask, add 5000ml of acetone and 0.1M acetic acid mixed extract, stir and extract at room temperature for 2 hours, then filter, and wash the filter residue three times with a small amount of mixed extract. The filtrate was concentrated to dryness at 40°C under reduced pressure, added 500ml of 50% alcohol to dissolve the solid, and hydrolyzed in a water bath at 80°C for 15 hours. The hydrolyzed product was isoflavone glycosides. Concentrate under reduced pressure at 65°C until alcohol-free. The suspension passes through a φ3.0×50cm polyamide chromatography column at a flow rate of 5ml / min, and the column is rinsed with water after the suspension is exhausted until the effluent is a colorless transparent liquid. The chromatography column was eluted with 500ml (about twice the column volume) of 70% alcohol. The received 5...

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Abstract

A process for extracting isoflavone from soybean includes solvent extraction, vacuum concentration, and column chromatography separation to obtain high-purity soybean isoflavone, and is mainly characterized by using two-step hydrolysis to extract soybean's isoflavone glycoside, isoflavone glycosyl, soybean extract and dye lignin separatedly, which can be used as raw materials of health-care food and medicines.

Description

1. Technical field [0001] The invention relates to a method for extracting isoflavones in soybeans, especially for high-quality soybeans with high isoflavone content, high-purity isoflavones can be obtained by various biochemical means such as solvent extraction, vacuum concentration, hydrolysis, and column chromatography separation . 2. Background technology [0002] In recent years, American scientists have discovered that a unique anti-cancer substance, the crystals of trihydroxyisoflavone (isoflavone hydrolyzate), can effectively block the physiological process of angiogenesis during the breeding of malignant tumors, cut off the source of nutrients, and thus Delay or prevent the development of tumor lesions. The American Academy of Nutrition and Foodstuffs investigated the recipes of Americans and Japanese and found that eating soy food is the main reason for the low incidence of cancer in Japan, especially the incidence of breast cancer and prostate cancer. Americans a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40
Inventor 冯国洲
Owner INST OF ZOOTCHNICS & VERTERINARY SCI BEIJING ACAD OF AGRI & FORESTRY SCI
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