42results about How to "Applicable separation" patented technology

The invention provides a method for rapidly separating auricularia auricula strains by the utilization of dry auricles. The method includes the steps that (1), the dry auricles are broken off with the hands to be small pieces; (2), the small auricles are washed in sterile water for the first time, and moisture is sufficiently absorbed through absorbent paper; (3), the small auricles are placed into alcohol with the 75% volume fraction, soaked for 20-25seconds and then sufficiently absorbed through absorbent paper; (4), the small auricles are washed in the sterile water for the second time and sufficiently absorbed through absorbent paper; (5), the small auricles are inserted into a plate PDA culture medium for cultivation and observation at constant temperature; (6), germinated hyphae are picked to be inoculated to a slope PDA culture medium and then cultivated in a pure mode. The method for separating the auricularia auricular strains has the advantages of being easy and convenient to operate, short in consumed time and low in needed cost, the hyphae are high in survival rate, rapid in growth, pure white, sturdy and high in vitality, the application range is wide, and the method is suitable for separating kinds of colloid auricularia auricula strains.

The invention provides a preparation method for a monoamine-grafted-and-modified crosslinked polyimide solvent-resistant nanofiltration membrane. The preparation method comprises the following steps: a, preparing a mixed solution from a solvent and a cosolvent according to a mass ratio of 1: 0.1-5; b, adding 15 to 28% of a polyimide solution into the mixed solution so as to prepare a membrane casting liquid; c, adding 2.5 to 20% of monoamine into the membrane casting liquid and carrying out mixing at 25 to 80 DEG C for 3 to 24 h under stirring so as to guarantee complete grafting; d, preparing a membrane by using a immersion-precipitation phases inversion process, washing the prepared membrane with deionized water a plurality of times and standing the washed membrane for subsequent usage; e, preparing an alcoholic solution of diamine with a concentration of 0.5 to 20%; f, putting the prepared membrane in the alcoholic solution of diamine prepared in the previous step for crosslinking modification for 0.25 to 48 h; and g, after completion of the reaction, soaking and cleaning the membrane in an alcohol solvent so as to obtain the polyamide nanofiltration membrane with excellent performance. The polyimide solvent-resistant nanofiltration membrane prepared in the invention has the advantages of simple preparation, adjustable hydrophilicity, outstanding solvent resistance, great permeation flux, a high retention rate, etc. and is applicable to separation of substances in all the organic solvents and to the fields of pharmaceutical manufacturing, biology, catalyst recovery, etc.