Modeling and detecting method for rapid detection on nitrate in vegetables

Abstract

The invention relates to a modeling and detecting method for on-scene safe and rapid detection on nitrate in vegetables based on an infrared total-reflection spectrum of a screened range. The method comprises the steps: dividing the vegetables into two parts, measuring experimental control data of one part by a chemical method, and extracting characteristic absorption spectrograms with the wave number of 6.5 to 8 microns from the other part as modeling samples; preparing a series of nitrate standard solutions, and acquiring spectral information as modeling samples; subjecting two curves to preprocessing, so as to remove interference; modeling a spectral curve of the nitrate in the vegetables, a spectral curve of the standard solutions and chemical reference values so as to establish correlativity; acquiring characteristic absorption spectrograms of the vegetables to be detected, and subjecting spectra to preprocessing so as to remove interference; subjecting the spectral curve of the nitrate and models to intelligent identification classifying, selecting optimal-matched modeling samples and modeling sample numbers, acquiring samples with optimal similar characteristics through an intelligent algorithm, predicting nitrate content, and carrying out comparison, so as to obtain edible information on the vegetables. The method is safe, does not use chemical reagents, is applicable to on-scene rapid acquisition and is high in detection efficiency.

Description

technical field [0001] The invention relates to a modeling method and a detection method for the detection of nitrate in food, in particular to a rapid quantitative determination method of nitrate based on infrared spectrum, which belongs to the field of agricultural food detection. Background technique [0002] With the improvement of residents' living standards and the development of science and technology, food health has attracted more and more attention. Studies have shown that nitrate can be reduced to nitrite after conversion in the human body. Nitrite is a strong carcinogen. Continuous intake of 0.3-0.5g of nitrite can cause poisoning, and intake of 3.0g of nitrite can cause death ; Nitrate enters the human body and is reduced to nitrite by gastrointestinal bacteria. Excessive nitrite can induce cancer in the human digestive system. On the one hand, the factors that increase the potential harm of human body intake of nitrate are the increasingly serious nitrate poll...

AI Technical Summary

Problems solved by technology

Among them, colorimetry, ultraviolet spectrophotometry, ion chromatography, and ion-specific electrode methods require special equipment and professional testing personnel, and the sample pretreatment steps are cumbersome; the kit method requires the use of chemical reagents, which are obtained through color ratio comparison. For the results of nitrate within a certain range, the above methods are not operable in the popularization of detection, which restricts the application of rapid detection of nitrate in vegetables and drinking water in terms of market demand and popularity

At present, the market lacks detection equipment and methods that do not use chemical reagents, are simple and fast to operate, are safe on site, and quickly detect nitrate in vegetables

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