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A set of primers to identify the progeny of hybrids between Fengyue and Chaohong kiwifruit varieties

A technology for kiwifruit and Fengyue, which is applied in the field of plant genetics and breeding, can solve the problems such as the lack of obvious species characteristics, and achieve the effects of saving breeding costs, not being affected by the environment, and having good repeatability.

Active Publication Date: 2019-09-03
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Description
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  • Application Information

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Problems solved by technology

However, the early species characteristics of kiwifruit hybrid progenies are not obvious, and it is impossible to identify whether it is a real interspecific hybrid by simple trait discrimination

Method used

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  • A set of primers to identify the progeny of hybrids between Fengyue and Chaohong kiwifruit varieties
  • A set of primers to identify the progeny of hybrids between Fengyue and Chaohong kiwifruit varieties

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Embodiment Construction

[0035] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0036] The materials used in the examples include: one sample each of the parents Fengyue and Chaohong used for cross breeding, and three hybrid offspring with breeding potential.

[0037] 1. Development of chloroplast and mitochondrial molecular markers

[0038] The chloroplast and mitochondrial genome sequences of Fengyue and Chaohong were linearly compared, and the chloroplast and mitochondrial genome variation sites were identified; primers were designed for 3 chloroplast and mitochondrial genome variation sites respectively, and the design parameters and requirements were as follows: (1 ) The distance between the upstream and downstream primer sequences is at least 100bp; (2) The length of the primer sequence is 19-23bp; (3) The GC content of the primer sequence is 40%-60%; (4) The length of the amplicon is 600-1000bp; 3 pairs of pri...

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Abstract

The invention relates to a primer for identifying hybrid generations between Fengyue and Chaohong kiwifruit varieties and belongs to the field of genetic breeding of plants. The primer comprises three chloroplast primer pairs and three mitochondria primer pairs: SEQ ID NO: 7 and SEQ ID NO: 8, SEQ ID NO: 9 and SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, SEQ ID NO: 13 and SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16 and SEQ ID NO: 17 and SEQ ID NO: 18. The method comprises the following steps: (1) extracting genome DNA (Deoxyribonucleic Acid); (2) carrying out PCR (Polymerase Chain Reaction) amplification; (3) carrying out DNA sequencing; (4) carrying out sequence analysis; and (5) identifying the hybrid generations. Compared with the prior art, the primer is simple to operate, has good repeatability and high stability and is not influenced by the environment; and the culture cost is saved.

Description

technical field [0001] The invention belongs to the field of plant genetics and breeding, in particular to a set of primers for identifying hybrid offspring between Fengyue and super red kiwifruit varieties, which can speed up the kiwifruit breeding process. Background technique [0002] Kiwi fruit (genus name: Actinidia) is a dioecious large deciduous woody vine plant. Its fruit is soft in texture, sweet and sour in taste, rich in nutritional value, rich in vitamins, calcium, potassium, selenium, zinc, germanium and other trace elements. Known as the "King of Fruits". At present, my country's kiwifruit production and area both rank first in the world, and play a pivotal role in the world's kiwifruit industry. [0003] There are 52 species of kiwifruit native to my country, and the species have a wide range of fruit phenotypes, quality characteristics and rich diversity of genetic variation. The species with greater cultivation value in production are Actinidia chinensis, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 钟彩虹赵婷婷李大卫陈美艳刘小莉韩飞
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI