Method for obtaining homozygous tetraploid winter jujube plants by inducing callus and adventitious buds on leaves

Abstract

The invention discloses a method for obtaining pure tetraploid jujube plants by inducing callus and adventitious buds through leaves. The method establishes a regeneration system of isolated leaves of winter jujube, and optimizes basic medium, plant growth regulators, AgNO 3 Additions and other conditions were used to establish a high-efficiency regeneration system for the isolated leaves of the chimera 'Zhandong 2', and to induce clusters of adventitious buds on the leaves. The method of combining morphological index determination, cytological observation of chromosome number, and flow cytometry detection of cell DNA was also used. , identified the ploidy of adventitious buds regenerated from leaves, screened out the homozygous tetraploid 'Zhandong 2', and carried out rapid propagation on it. Tetraploid winter jujube plants are obtained through the purification of natural bud change ploidy chimeras, which can be directly transplanted into the field without undergoing transitional culture, greenhouse cultivation, transplanting to the ground like in vitro tissue culture, and can be used as renewable materials in the field The advantages of rapid evaluation improved the purification efficiency of 'Zhandong 2' and accelerated the acquisition of pure tetraploid Dongzao.

Description

technical field [0001] The invention relates to a plant breeding method, in particular to a method for obtaining pure tetraploid jujube plants by inducing callus and adventitious buds from leaves. Background technique [0002] Jujube is native to China and is one of the most distinctive and advantageous fruit tree species in China. The cultivated area is nearly 30 million mu, and the output is more than 6 million tons, accounting for 99% of the world. In recent years, with the development of jujube industry, the structure of cultivated varieties has been continuously adjusted and optimized, and the cultivation of fresh jujube has been paid more and more attention. 'Dongzao' is currently my country's largest variety of high-quality fresh jujubes. In addition to Shandong Zhanhua, it has also formed a certain scale in Hebei, Shanxi and Shaanxi. However, the fruit size of 'Dongzao' is relatively small and the cultivation and management requirements are high. The genetic improve...

AI Technical Summary

Problems solved by technology

'Zhandong 2' currently planted in a large area in the field is a ploidy chimera material (dimixed and tetramixed) derived from the natural variation of 'Dongzao', and its growth characteristics are unstable. A considerable proportion of diploid 'Dongzao' will appear every year, and its resistance is low. In recent years, frost damage has been severe, which increases the difficulty of management, reduces the income, seriously affects the enthusiasm of jujube farmers, and limits the promotion of this variety. develop

In the innovation of jujube ploidy germplasm, there have been reports of artificial mutagenesis and endosperm culture to obtain polyploidy, but the purification of natural bud change ploidy chimeras has not been reported