A kind of preparation method of cyclodextrin polymer drug-loaded vesicle

A cyclodextrin polymer and drug-carrying technology, which is used in pharmaceutical formulations, non-active components of polymer compounds, and medical preparations with non-active components, etc. Low problems, to achieve the effect of strong drug-carrying capacity, simple preparation process, easy operation and realization

Active Publication Date: 2020-06-26
南京紫鸿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Cancer is one of the major problems facing the medical field today. Chemotherapy is an indispensable and important means in the comprehensive treatment of cancer. However, traditional chemotherapy methods have disadvantages such as high toxicity and side effects, low bioavailability of drugs, and lack of specific recognition. Its application and development in cancer treatment

Method used

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  • A kind of preparation method of cyclodextrin polymer drug-loaded vesicle
  • A kind of preparation method of cyclodextrin polymer drug-loaded vesicle
  • A kind of preparation method of cyclodextrin polymer drug-loaded vesicle

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A preparation method of cyclodextrin polymer drug-loaded vesicles, comprising the steps of:

[0038] 1) Dissolve CS-g-CD in 10ml of 1% (v / v) glacial acetic acid solution (pH=3) to prepare a solution with a concentration of 1mg / mL, and dissolve BM-PCL in 10ml of DMF to prepare A solution with a concentration of 0.1 mg / mL was obtained;

[0039] 2) Add 6.7 mg of doxorubicin to the BM-PCL solution and stir to dissolve, then add it dropwise to the CS-g-CD solution at a rate of 1-2 drops / s under stirring conditions, and continue stirring after the addition is complete After 24 hours, dialyze in distilled water for 6 hours to obtain polymer drug-loaded vesicles with a drug loading capacity of 37.8%.

Embodiment 2

[0041] A preparation method of cyclodextrin polymer drug-loaded vesicles, comprising the steps of:

[0042] 1) Dissolve CS-g-CD in 20ml of 1% (v / v) glacial acetic acid solution (pH=3) to make a solution with a concentration of 0.1mg / mL, and dissolve BM-PCL in 5ml of DMF Prepare a solution with a concentration of 0.1 mg / mL;

[0043] 2) Add 1.7 mg of naproxen to the BM-PCL solution and stir to dissolve, then add dropwise to the CS-g-CD solution at a rate of 1-2 drops / s under stirring conditions and mix, continue to Stir for 48 hours and dialyze in distilled water for 4 hours to obtain polymer drug-loaded vesicles with a drug loading capacity of 39.8%.

Embodiment 3

[0045] A preparation method of cyclodextrin polymer drug-loaded vesicles, comprising the steps of:

[0046] 1) Dissolve CS-g-CD in 50ml of 1% (v / v) glacial acetic acid solution to make a solution with a concentration of 0.1mg / mL, and dissolve BM-PCL in 5ml of DMF to make a concentration of 0.5 mg / mL solution;

[0047] 2) Add 10.3 mg of indomethacin into the BM-PCL solution and stir to dissolve, and then add it dropwise to the CS-g-CD solution at a rate of 1-2 drops / s under stirring conditions and mix. Stirring was continued for 12 hours and dialyzed in distilled water for 8 hours to obtain polymer drug-loaded vesicles with a drug loading capacity of 37.1%.

[0048]In order to verify the drug controlled release and sustained release effect of the polymer drug-loaded vesicle obtained in the present invention, we have carried out the test of the controlled release and sustained release effect of the drug, and the specific results are as follows image 3 Shown:

[0049] Depend ...

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Abstract

The invention relates to a preparation method of a cyclodextrin-polymer drug-loaded vesicle. The preparation method comprises the following steps: adopting chitosan-grafted cyclodextrin polymer and benzimidazole polycaprolactone as materials, respectively dissolving CS-g-CD and BM-PCL in a solvent to prepare a solution; adding drugs into the BM-PCL solution obtained in step 1), stirring and dissolving, then dripping the obtained BM-PCL solution into CS-g-CD solution under the stirring condition and at a speed of 1-2 drops / s, continuing to stir after the dripping is finished, carrying out distilled-water dialysis, and thus obtaining polymer drug-loaded vesicle. The preparation method has the advantages that the prepared polymer vesicle has strong drug-loading capability for hydrophilic and hydrophobic drugs and can realize high-efficiency loading and controlled release of the drugs; and the polymer vesicle has a hollow structure and is 100-200nm in particle size, and the size of the vesicle can be adjusted according to the proportion of two polymers, namely CS-g-CD and BM-PCL.

Description

technical field [0001] The invention relates to a method for preparing a drug carrier, in particular to a method for preparing a polymer vesicle, and belongs to the technical field of drug carriers. Background technique [0002] Cancer is one of the major problems facing the medical field today. Chemotherapy is an indispensable and important means in the comprehensive treatment of cancer. However, traditional chemotherapy methods have disadvantages such as high toxicity and side effects, low drug bioavailability, and lack of specific recognition. Its application and development in cancer therapy. [0003] Polymeric vesicles have many unique physicochemical properties, which have great application value in the field of cancer therapy. Polymer vesicles are a type of aggregates with a double-layer membrane structure formed by self-assembly of amphiphilic blocks or graft copolymers in aqueous solution. They have large internal capacity, high stability, and can load hydrophilic ...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/36A61K47/40A61K47/34A61K47/22A61K47/61A61K47/69A61P35/00A61K31/513A61K31/704A61K31/192A61K31/405A61K31/196
CPCA61K31/192A61K31/196A61K31/405A61K31/513A61K31/704A61K47/22A61K47/34A61K47/36A61K47/40
Inventor 李桂英高玉荣郭磊周在帅高玲玲
Owner 南京紫鸿生物科技有限公司
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