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Resolving technology for purifying glutathione metal salt

A technology of glutathione and metal salt, applied in the field of medicine, can solve the problems of high price, high cost, high production cost, and achieve the effects of less storage space, low production cost and improved safety

Active Publication Date: 2017-08-18
云南积大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the reaction between hydrogen sulfide and the complex is limited, and the final yield of the reaction is about 85% of the theoretical value, and about 10% of the reduced glutathione has not been resolved, resulting in a certain waste
[0008] At the same time, hydrogen sulfide is used as a desorption agent, and its price is relatively high during use, resulting in high production costs; when hydrogen sulfide is used for desorption reaction, the yield of glutathione is only 85%, which further increases the production cost. In large-scale production where production cost is more critical, it will lead to a substantial increase in product cost
[0009] Sodium sulfide is solid, if it can be used as a desorbent, it can solve some disadvantages of hydrogen sulfide gas, such as not easy to store, high toxicity, etc.
According to the mechanism of chemical reaction, it can be considered to directly replace hydrogen sulfide with sodium sulfide to solve the above-mentioned shortcomings in the production process of hydrogen sulfide. However, sodium sulfide is a strong alkali substance, and adding it to the complex will greatly increase the pH of the solution. If the reaction speed is not fast enough , the generated product reduced glutathione will be catalyzed by dissolved oxygen under the catalysis of unreacted metal ions such as copper ions within a certain period of time to produce oxidized glutathione, resulting in a decrease in yield and purity, so sodium sulfide The analytical method to replace hydrogen sulfide has not been reported or patented so far, which requires the invention of a solid reagent to speed up the reaction, so that the resolved metal ions are quickly complexed by sulfur ions in a very short period of time, and cannot play a catalytic role. , so as to solve the problems of poor safety, low yield and high cost of hydrogen sulfide

Method used

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  • Resolving technology for purifying glutathione metal salt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: When hydrogen sulfide is used as a resolving agent, the reduced glutathione cuprous is analyzed:

[0028] Cuprous glutathione is dispersed in purified water, with a total volume of 1100L, a glutathione content of 1.33%, and a total glutathione content of 14.63kg. 15°C-25°C, hydrogen sulfide gas is introduced under stirring at a flow rate of about 2kg / h, and the color of the solution turns from milky white to black. After 30 minutes, the content of glutathione in the analysis solution was sampled and detected to be 1.15%. Stop the introduction of hydrogen sulfide, close and stir for 1 hour, adjust the pH of the analysis solution to 4.0 ± 0.2 with 10% sodium hydroxide solution or hydrochloric acid, take a sample to detect that the content of glutathione in the analysis solution is 1.17%, and the purity is 93.7%. The rate is 85.3%. The actual use of hydrogen sulfide in measuring hydrogen sulfide cylinders by weight loss method is 0.97kg, and the cost of des...

example 2

[0029] Example 2: When sodium sulfide solid is used as a resolving agent, cuprous glutathione is resolved:

[0030] Cuprous glutathione is dispersed in purified water, with a total volume of 1100L, a glutathione content of 1.38%, and a total glutathione content of 15.18kg. Add 2.03kg of solid sodium sulfide under stirring at 15°C-25°C. After 10 minutes, the color of the solution turns black. Continue to stir until 30 minutes later, adjust the pH of the analysis solution to 4.0±0.2 with 10% sodium hydroxide solution or hydrochloric acid, and take a sample to detect that the content of glutathione in the analysis solution is 1.23%, and the purity is 85.2 %, yield 84.1%. A total of 2.03kg of sodium sulfide was added, and the cost of the desorbing agent was ￥30.25. The purity was lower than that of hydrogen sulfide as the desorbing agent, which brought inconvenience to the subsequent refining.

example 3

[0031] Example 3: When a mixed solid of sodium sulfide and sodium chloride (10%) is used as a resolving agent, cuprous glutathione is resolved:

[0032] Cuprous glutathione is dispersed in purified water, with a total volume of 1100L, a glutathione content of 1.40%, and a total glutathione content of 15.40kg. Add 2.29kg of mixed solids of sodium sulfide and sodium chloride under stirring at 15°C-25°C, and the proportion of sodium chloride is 10%. After 5 minutes, the color of the solution turns black. Continue stirring until 30 minutes later, adjust the pH of the analysis solution to 4.0±0.2 with 10% sodium hydroxide solution or hydrochloric acid, and take a sample to detect that the content of glutathione in the analysis solution is 1.33%, and the purity is 92.4 %, the yield is 95.0%. The input amount of sodium sulfide is 2.061kg, sodium chloride is 0.229kg, and the cost of desorbent is about ￥30.78.

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Abstract

The invention belongs to the technical field of medicines, and relates to a resolving technology for reducing type glutathione metal salt. The process comprises the following steps: by taking a sodium sulfide mixture containing 10 to 50 percent of sodium chloride as a resolving agent, replacing hydrogen sulfide gas, performing reaction on the sodium sulfide mixture and the glutathione metal salt, and resolving glutathione. Compared with a process in which hydrogen sulfide is added, the resolving process has the advantages that the safety is improved, and the great potential safety hazard in transportation, storage and use processes of the hydrogen sulfide is avoided; the resolving agent in the process can effectively improve the reaction yield; a sodium sulfide and sodium chloride mixed solid is cheaper than the hydrogen sulfide, so that the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a process for analyzing glutathione metal salts. Background technique [0002] Glutathione (glutathione, r-glutamyl cysteinyl glycine, GSH) is a tripeptide containing γ-amide bonds and sulfhydryl groups, composed of glutamic acid, cysteine ​​and glycine. Present in nearly every cell of the body. Glutathione helps maintain normal immune system function and has antioxidant and integrative detoxification effects. [0003] Currently, the existing process of glutathione is produced by fermentation. After the production is over, the yeast cell wall is broken, and then glutathione is obtained by extraction. The solution obtained after breaking the cell wall contains a large amount of cell components, fermentation broth and microorganisms, etc. The existing glutathione purification technology uses metal salts to convert glutathione in the multi-impurity extract into glutathione Peptide ...

Claims

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Application Information

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IPC IPC(8): C07K5/037
CPCC07K5/0215
Inventor 赖涛程雪飞杜凌琳王立江刘小龙张晓腾义君
Owner 云南积大生物科技有限公司