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Method for preparing chitosan oligosaccharide from chitosan

A technology of chitin oligosaccharides and chitin, applied in the biological field, can solve the problems of unrecoverable acid and alkali, cumbersome steps, and many steps, and achieve the effects of high environmental protection efficiency, simple reaction conditions, and good reproducibility

Inactive Publication Date: 2017-08-18
杭州垚信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The disadvantages of the above steps: the raw material chitin must first be deacetylated from chitin to obtain chitosan, and chitosan can not be produced in one step. There are many steps, and if chitosan is used as raw material, it is too expensive; After repeated filtration and washing, the steps are cumbersome, and a large amount of acid and alkali cannot be recovered, which increases the production cost and causes environmental pollution; there are unreacted long-chain polysaccharides in the produced chitosan oligosaccharide solution, and the product is impure

Method used

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  • Method for preparing chitosan oligosaccharide from chitosan

Examples

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Effect test

Embodiment 1

[0020] 1) Weigh 100g of chitin, dissolve it in 1L of water, place in a water bath at 50°C, stir constantly, adjust the pH to 8.5 with 10% sodium hydroxide solution, add 0.1g of chitin deacetylase, and react at constant temperature for 6 hours to obtain chitosan sugar solution;

[0021] 2) Add 31% hydrochloric acid to adjust the pH to 5.5. It can be seen that most of the chitosan precipitates are dissolved in water to form a high-viscosity solution. Add 0.1 g of chitosanase and 0.1 g of cellulase, and stir in a water bath at 50 ° C for 2 hours to obtain Chitosan oligosaccharide solution;

[0022] 3) Add 10% sodium hydroxide to adjust the pH to 8.2, filter to obtain undegraded chitosan residue and degraded chitosan solution, and now the degraded chitosan solution is a light yellow clear liquid, and the above-mentioned undegraded The chitosan residue is filtered, joins in the next batch of preparation method step 2), circulates back and forth, to improve the productive rate of c...

Embodiment 2

[0026] 1) Weigh 100g of chitin, dissolve it in 1L of water, place in a water bath at 50°C, stir constantly, adjust the pH to 8.5 with 10% sodium hydroxide solution, add 0.1g of chitin deacetylase, and react at constant temperature for 6 hours to obtain chitosan sugar solution;

[0027] 2) Add 31% hydrochloric acid to adjust the pH to 5.5. It can be seen that most of the chitosan precipitates are dissolved in water to form a high-viscosity solution. Add 0.1 g of chitosanase and 0.1 g of cellulase, and stir in a water bath at 50 ° C for 2 hours to obtain Chitosan oligosaccharide solution;

[0028] 3) Add 10% sodium hydroxide to adjust the pH to 8, filter to obtain undegraded chitosan residue and degraded chitosan solution, the degraded chitosan solution is light yellow clear liquid, the above-mentioned undegraded The chitosan residue is filtered, joins in the next batch of preparation method step 2), circulates back and forth, to improve the productive rate of chitosan oligosac...

Embodiment 3

[0032] 1) Weigh 100g of chitin, dissolve it in 1L of water, place in a water bath at 50°C, stir constantly, adjust the pH to 8.5 with 10% sodium hydroxide solution, add 0.1g of chitin deacetylase, and react at constant temperature for 6 hours to obtain chitosan sugar solution;

[0033] 2) Add 31% hydrochloric acid to adjust the pH to 5.5. It can be seen that most of the chitosan precipitates are dissolved in water to form a high-viscosity solution. Add 0.1 g of chitosanase and 0.1 g of cellulase, and stir in a water bath at 50 ° C for 2 hours to obtain Chitosan oligosaccharide solution;

[0034] 3) Add 10% sodium hydroxide to adjust the pH to 8.5, filter to obtain undegraded chitosan residue and degraded chitosan solution, the degraded chitosan solution is light yellow clear liquid, the above-mentioned undegraded The chitosan residue is filtered, joins in the next batch of preparation method step 2), circulates back and forth, to improve the productive rate of chitosan oligos...

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Abstract

The invention discloses a method for preparing chitosan oligosaccharide from chitosan. The method comprises the following steps: 1) taking chitosan as a raw material, dissolving in water, continuously stirring, regulating pH of a sodium hydroxide solution to 8.5, adding chitin deacetylase at 50 DEG C, and reacting for 6 hours at constant temperature, thereby acquiring a chitosan solution; 2) adding hydrochloric acid for regulating pH to 5.5, adding chitosanase and cellulose, and stirring for 2 hours at the constant temperature of 50 DEG C, thereby acquiring a chitosan oligosaccharide solution; 3) regulating pH to be alkaline, thereby acquiring undegraded chitosan residues and a degraded chitosan oligosaccharide solution, filtering the undegraded chitosan residues, and returning into the next reaction; and 4) distilling and concentrating the degraded chitosan oligosaccharide solution so as to acquire a concentrated solution, adding ethyl alcohol, filtering and drying, thereby acquiring chitosan oligosaccharide. According to the preparation method, the reaction conditions are simple, and the steps are few; the efficiency is high, and the industrial large-scale production is benefited; and a relatively pure chitosan oligosaccharide product is acquired.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preparing chitin into chitosan oligosaccharide. Background technique [0002] Oligosaccharides are also called chitosan oligosaccharides and chitosan oligosaccharides, which are obtained by degrading chitosan through special biological enzyme technology (there are also reports of chemical degradation and microwave degradation technology) with a degree of polymerization between 2 and 20. Meta-oligosaccharide products, molecular weight ≤ 3200Da, are low molecular weight products with good water solubility, great function and high biological activity. It has many unique functions such as higher solubility than chitosan, fully soluble in water, and easy to be absorbed and utilized by organisms. [0003] At present, the method for preparing chitosan oligosaccharides comprises the following steps: prepare chitosan oligosaccharides with chitin, add chitin to 50% concentrated s...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/12C12P19/14C08B37/08C07H3/06C07H3/04C07H1/06
CPCC07H1/06C07H3/04C07H3/06C08B37/0003C08B37/003C12P19/04C12P19/12C12P19/14
Inventor 夏雪青
Owner 杭州垚信生物科技有限公司
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