Method for simultaneously enriching and detecting phenolic, estrogenic and androgenic EDCs (Endocrine Disrupting Chemicals) in drinking water
A technology for endocrine disruptors and drinking water, which is applied in the direction of measuring devices, material separation, and analysis of materials, to achieve the effects of reducing detection costs, strong selectivity, and saving sampling time
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[0076] Example 1
[0077] A method for simultaneous enrichment and detection of phenols, estrogen and androgen endocrine disruptors in drinking water. The specific steps for preparing the standard working curve are as follows:
[0078] (a) Prepare mixed standard stock solution: mix three types of EDCs with known concentrations with 50% methanol / water solution (v / v) to prepare a mixed standard stock solution with the same concentration of single EDCs, and place them in a brown reagent bottle;
[0079] (b) Dilute the mixed standard stock solution in step (a) with 50% methanol / water solution (v / v) to different concentration gradients: 1μg / L, 5μg / L, 10μg / L, 20μg / L, 50μg / L, 100μg / L, 200μg / L, 500μg / L and 1000μg / L, and add 200μg / L BPA-D16, 200μg / L E2-D2 and 100μg / L TES-D3 to each concentration gradient sample at the same time;
[0080] (c) Use LC-MS / MS to analyze each concentration gradient sample, use the above optimized liquid chromatography operating parameters and mass spectrometry opera...
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[0086] Example 2
[0087] A method for the simultaneous enrichment and detection of phenols, estrogen and androgen endocrine disruptors in drinking water includes the following steps:
[0088] (1) Sample pretreatment:
[0089] Select a water sample from a water source in East China, use a 0.7μm glass fiber membrane as a filter membrane to remove suspended solids, adjust the pH to 5.0 and add complexing agent Na 2 EDTA, and then add a known amount of three types of internal standards: 200μg / L BPA-D16, 200μg / L E2-D2 and 100μg / L TES-D3 to complete the pretreatment;
[0090] (2) Concentration and enrichment of target EDCs:
[0091] First use 5-10mL equal volume of methanol, ultrapure water and dilute hydrochloric acid solution to activate by passing through SPE cartridge (Oasis HLB solid phase extraction cartridge), and then take 500mL of the water sample pretreated in step (1) and pass the column extraction After enrichment, take 5% methanol / water solution (v / v) as the eluent to wash the ...
Example Embodiment
[0099] Example 3
[0100] A method for the simultaneous enrichment and detection of phenols, estrogen and androgen endocrine disruptors in drinking water includes the following steps:
[0101] (1) Sample pretreatment:
[0102] Select a water sample from a water source in East China, use a 0.7μm glass fiber membrane as a filter membrane to remove suspended solids, adjust the pH to 5.0 and add complexing agent Na 2 EDTA, and then add a known amount of three types of internal standards: 200μg / L BPA-D16, 200μg / L E2-D2 and 100μg / L TES-D3 to complete the pretreatment;
[0103] (2) Concentration and enrichment of target EDCs:
[0104] First use 5-10mL equal volume of methanol, ultrapure water and dilute hydrochloric acid solution to activate by passing through SPE cartridge (Oasis HLB solid phase extraction cartridge), and then take 500mL of the water sample pretreated in step (1) and pass the column extraction After enrichment, take 5% methanol / water solution (v / v) as the eluent to wash the ...
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