A photocatalyst medium capable of improving the survival rate of Polygonatum explants and its preparation method

An explant and survival rate technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of low survival rate, complex and changeable wild growth environment, cumbersome seed propagation, etc., and achieve accelerated growth , promote metastasis, and promote rapid growth

Active Publication Date: 2019-07-19
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the standardized cultivation of Rhizoma Polygonatum has just started, the wild growth environment is complex and changeable, and there is no ready-made experience for cultivation management measures.
As the source plant of Polygonatum Polyflora, Polygonatum multiflora rhizomes have a low reproductive coefficient, while seed propagation is cumbersome, and the survival rate is not high, and the growth process is easily affected by the external environment.

Method used

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  • A photocatalyst medium capable of improving the survival rate of Polygonatum explants and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a photocatalyst culture medium that can increase the survival rate of Polygonatum explants, which is composed of the following substances in terms of weight ratio:

[0029] 0.1g boric acid, 0.2g sodium carbonate, CoCl 2 0.1g, inositol 0.2g, CuSO 4 0.07g, ammonium nitrate 0.8g, potassium dihydrogen phosphate 0.4g, sodium chloride 0.8g, glucose 1.2g, nano titanium dioxide 0.2g, nano zinc oxide 0.2g, calcium chloride 0.2g, glycine 0.04g, BA 0.04g, NAA 0.01g, IAA 0.02g, KT 0.013g, IBA 0.01g, Vitamin B1 0.02g, Vitamin B2 0.02g, 0.2g magnesium sulfate heptahydrate, and 80g purified water.

Embodiment 2

[0040] This embodiment provides a culture medium that can increase the survival rate of Polygonatum explants, which is composed of the following substances in terms of weight ratio:

[0041] 0.15g boric acid, 0.25g sodium carbonate, CoCl 2 0.15g, inositol 0.3g, CuSO 4 0.08g, ammonium nitrate 1.0g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.0g, glucose 1.3g, nano titanium dioxide 0.25g, nano zinc oxide 0.25g, calcium chloride 0.25g, glycine 0.05g, BA 0.045g, NAA 0.015g, IAA 0.025g, KT 0.012g, IBA 0.015g, vitamin B1 0.03g, vitamin B2 0.03g, 0.3g magnesium sulfate heptahydrate, and 90g purified water.

Embodiment 3

[0052] This embodiment provides a culture medium that can increase the survival rate of Polygonatum explants, which is composed of the following substances in terms of weight ratio:

[0053] 0.2g boric acid, 0.3g sodium carbonate, CoCl 2 0.2g, inositol 0.4g, CuSO 4 0.09g, ammonium nitrate 1.2g, potassium dihydrogen phosphate 0.6g, sodium chloride 1.2g, glucose 1.4g, nano titanium dioxide 0.3g, nano zinc oxide 0.3g, calcium chloride 0.3g, glycine 0.06g, BA 0.05g, NAA 0.02g, IAA 0.03g, KT 0.015g, IBA 0.02g, Vitamin B1 0.04g, Vitamin B2 0.04g, 0.4g magnesium sulfate heptahydrate, and 100g purified water.

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Abstract

The invention discloses a photocatalyst medium capable of improving a survival rate of a rhizoma polygonati explant. The medium comprises the following components in grams by weight: 0.1-0.2g of boric acid, 0.2-0.3g of sodium carbonate, 0.1-0.2g of CoCl, 0.2-0.4g of inositol, 0.07-0.09g of CuSO4, 0.8-1.2g of ammonium nitrate, 0.4-0.6g of monopotassium phosphate, 0.8-1.2g of sodium chloride, 1.2-1.4g of glucose, 0.1-0.5g of photocatalyst, 0.2-0.3g of calcium chloride, 0.04-0.06g of glycine, 0.04-0.08g of vitamin, 0.2-0.4g of magnesium sulfate heptahydrate, 80-100g of purified water and 0.04-0.06g of a plant growth regulator. According to the medium disclosed by the invention, the survival rate of the rhizoma polygonati explant can be obviously improved.

Description

Technical field [0001] The invention belongs to the field of tissue culture, and specifically relates to a photocatalyst medium capable of improving the survival rate of Polygonatum explants and a preparation method thereof. Background technique [0002] Polygonatum multiflorum is a perennial herbaceous Polygonatum in the Liliaceae family. Its rhizome is a traditional bulk medicinal material in my country. It has the functions of invigorating qi, nourishing yin, invigorating the spleen, moisturizing the lung, and prolonging life. Since the research on the standardized cultivation of Polygonatum has just started, the wild growth environment is complex and changeable, and there is no ready-made experience for cultivation and management measures. As a medicinal source of Polygonatum, Polygonatum multiflorum has a low rhizome propagation coefficient, and seed reproduction is more complicated, and the survival rate is not high, and it is easily affected by the external environment dur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 吴学谦杨胜祥周国泉
Owner ZHEJIANG FORESTRY UNIVERSITY
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