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Preparation method and application of induced pluripotent stem cell secretin

A technology of pluripotent stem cells and secretin, applied in the biological field, can solve problems such as difficult cultivation, achieve good results, increase yield, and be suitable for large-scale production

Active Publication Date: 2017-09-08
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the market potential of secretin from induced pluripotent stem cells is great, it is difficult to cultivate it in large quantities, and there are not many reports so far

Method used

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  • Preparation method and application of induced pluripotent stem cell secretin
  • Preparation method and application of induced pluripotent stem cell secretin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 A preparation method of induced pluripotent stem cell secretin

[0023] The preparation method comprises the following steps:

[0024] S1 resuspended induced pluripotent stem cells to obtain induced pluripotent stem cell suspension;

[0025] S2 Distribute the induced pluripotent stem cell suspension obtained in step S1 on a three-dimensional carrier, and place the induced pluripotent stem cell culture medium IMDM / F12+2mg / mL human albumin+40μg / mL vitamin C+3mg / mL in a bioreactor In ginsenoside monomer Rb1, at 37°C, 5% CO 2 Induced pluripotent stem cells were obtained by culturing under conditions for 24 hours;

[0026] S3 In step S2, at the vigorous stage of induced pluripotent stem cell division, rinse the induced pluripotent stem cells and the three-dimensional carrier twice with 0.01M phosphate buffer (pH=7.3), and then add the basic culture medium IMDM / F12 and Phosphate buffer (volume ratio: 1:2) was continued to cultivate for 6h, and the supernatant in ...

Embodiment 2

[0028] Example 2 A preparation method of induced pluripotent stem cell secretin

[0029] The preparation method comprises the following steps:

[0030] S1 resuspended induced pluripotent stem cells to obtain induced pluripotent stem cell suspension;

[0031] S2 Distribute the induced pluripotent stem cell suspension obtained in step S1 on a three-dimensional carrier, and place the induced pluripotent stem cell culture medium IMDM / F12+3mg / mL human albumin+50μg / mL vitamin C+5mg / mL in a bioreactor In ginsenoside monomer Rb1, at 37°C, 5% CO 2 Induced pluripotent stem cells were obtained by culturing under conditions for 48 hours;

[0032] S3 In step S2, at the vigorous stage of induced pluripotent stem cell division, wash the induced pluripotent stem cells and the three-dimensional carrier with 0.01M phosphate buffer (pH=7.3) for 3 times, and then add the basal culture medium IMDM / F12 and Phosphate buffer (volume ratio: 1:4) was further cultivated for 15 hours, and the supernat...

Embodiment 3

[0034] Example 3 A preparation method of induced pluripotent stem cell secretin

[0035] The preparation method comprises the following steps:

[0036] S1 resuspended induced pluripotent stem cells to obtain induced pluripotent stem cell suspension;

[0037] S2 Distribute the induced pluripotent stem cell suspension obtained in step S1 on a three-dimensional carrier, and place the induced pluripotent stem cell culture medium IMDM / F12+4mg / mL human albumin+60 μg / mL vitamin C+7mg / mL in a bioreactor In ginsenoside monomer Rb1, at 37°C, 5% CO 2 Induced pluripotent stem cells were obtained by culturing under conditions for 72 hours;

[0038] S3 In step S2, at the vigorous stage of induced pluripotent stem cell division, wash the induced pluripotent stem cells and the three-dimensional carrier with 0.01M phosphate buffer (pH=7.3) for 3 times, and then add the basal culture medium IMDM / F12 and Phosphate buffer (volume ratio: 1:6) was continued to cultivate for 24 hours, and the sup...

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to a preparation method and application of an induced pluripotent stem cell secretin. The preparation method of the induced pluripotent stem cell secretin, provided by the invention, comprises treatment of induced pluripotent stem cells, proliferation of the induced pluripotent stem cells, acquisition, purification and concentration of the induced pluripotent stem cell secretin, that is, the induced pluripotent stem cell secretin is obtained by culturing the induced pluripotent stem cells, taking supernatant fluid, and performing filtration and concentration. The preparation method provided by the invention is simple and feasible, is suitable for large-scale production, and effectively improves the yield of the induced pluripotent stem cell secretin, and the induced pluripotent stem cell secretin prepared with the method provided by the invention can be effectively applied to health products and cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of induced pluripotent stem cell secretin. Background technique [0002] Induced pluripotent stem cells (iPS cells) were originally introduced by Japanese Shinya Yamanaka in 2006 using a combination of four transcription factors (Oct4, Sox2, Klf4 and c-Myc) into A cell type similar to embryonic stem cells obtained by reprogramming differentiated somatic cells. iPS cells can not only be used for differentiation and transplantation, but also can provide an in vitro disease model, so as to study the mechanism of disease formation, screen new drugs and develop new treatment methods. The establishment of human iPS cells is recognized as one of the most important scientific and technological advances in 2007. This technology can not only establish individual-specific pluripotent stem cell lines from somatic cells, but also solve the problem...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12N5/10A61K8/99A61Q19/00A23L33/10
CPCA23L33/10A23V2200/00A61K8/99A61K2800/10A61Q19/00C12N5/0696C12N2510/02C12P21/00A23V2200/30A23V2200/302A23V2200/324
Inventor 车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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